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The map label for this gene is celB [H]

Identifier: 52140357

GI number: 52140357

Start: 4993099

End: 4994406

Strand: Reverse

Name: celB [H]

Synonym: BCZK4903

Alternate gene names: 52140357

Gene position: 4994406-4993099 (Counterclockwise)

Preceding gene: 52140355

Following gene: 52140358

Centisome position: 94.22

GC content: 37.69

Gene sequence:

>1308_bases
ATGCAAAAGTTTATTGCATTCATGGAGAAATATATTGTTCCTGTCGCTGGTAAAATCGGATCGCAACGTCACTTAGCTGC
GATCCGTGACGGATTTATCGCAGTTATGCCACTTATTTTAGTTGGTGCACTGGCATCACTAATTAATGGTTTTCCATCTG
AAGCTTTCCAAGATTTCATGAAAGGTTTGTTTGGTGAAACGTGGAAACAAGTTGGCGGTGGAATGTGGACTGGTTCTTTC
GCGATTCTAGCACTAATCATAGCATTTACAACAAGTTATAACTTAGCAAAATCTTACGGCGTTGATGGTTTGTCAGCAGG
TATTATTTCATTTGGTGCGTTAATTATTCTTACGCCAACAACACCGAAAGAAGGCGGATTGAACTTAGCTTGGACAGGTG
CACAAGGGTTATTCGTAGCAATTATTGTGGCACTCCTTGTTACTGAGGTATTCCGTTTCTTTGTACAAAGAAATATTACT
TTTAAAATGCCTGATGGAGTACCACCAGCAGTTTTAAGATCTTTCGCAGCTATAGTTCCAGCATTTGTTATCTTAACAGT
AGTTGCAGGTATTCAATTAGCAGTGAAATTAGCTGGTACAAGTGTTCATGAATTTATCTTTAATACGATTCAATCACCAC
TGCAAAGTTTAGCAGGGACATTACCAAGTGCAATTGTTATTGTACTCCTTGTTCATCTTCTTTGGTTCTTCGGTTTACAT
GGTCCAAATATCGTTGGTGGTATTATTGAGCCGTTATACTTACCAGCATTAGAGAAAAATATGCAGTTATTCAAAGGTGG
CACATCTGCATTTGATGTTCCAAACATTGTTACAAAACCGTTCTTTGATACTTTCGTATATCTTGGTGGTTCTGGTGCAA
CATTAGCGTTCTTAGTAGTTGTATTACTTGTAGCAAAAAGTGCACAATTACGCGGTGTATCTCGTTTATCAATTGGTCCA
GGTGCGTTCAACATTAACGAACCAGTAATCTTTGGTACACCAATTATTTTAAATCCAATTTTATTCTTACCGTTTATCAT
AACACCAATTGTATTAGTAATTACTTCTTATACAGCTATATCGATTGGCTGGGTACCAAAAACAGTTGTGATGATTCCAT
GGGCAACACCACCAATTATTAGTGGTTATCTTGTAACAGGTGGACATCTTTCAGGGGCAATTCTACAGTTATTCAACTTT
GTAATTGCGATGGTAATCTATTATCCATTCGTTGTGTTATGTGACCGTTCAGTTGTTCGTACTGAAAAAGCAGCAGCACA
AGGAAATAACAACTCTGTACCTATGTAA

Upstream 100 bases:

>100_bases
GGAATCGACGTTATCAATCCAATGCACTACGGCATGATGAATGGAGAAGCAGTTTTAGATCACGCATTAACACTAAAAAA
ATAATTAGGGGGGAAGCGAA

Downstream 100 bases:

>100_bases
CATAATAATTGTTCTCACAGATAACATGATCTGTGAGAACAATTACCTTTATATCATGTTCAAAAAGATGATACTCATCT
ACAAGAACACCAAACTATCA

Product: PTS system, cellobiose-specific IIC component

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIC component [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQKFIAFMEKYIVPVAGKIGSQRHLAAIRDGFIAVMPLILVGALASLINGFPSEAFQDFMKGLFGETWKQVGGGMWTGSF
AILALIIAFTTSYNLAKSYGVDGLSAGIISFGALIILTPTTPKEGGLNLAWTGAQGLFVAIIVALLVTEVFRFFVQRNIT
FKMPDGVPPAVLRSFAAIVPAFVILTVVAGIQLAVKLAGTSVHEFIFNTIQSPLQSLAGTLPSAIVIVLLVHLLWFFGLH
GPNIVGGIIEPLYLPALEKNMQLFKGGTSAFDVPNIVTKPFFDTFVYLGGSGATLAFLVVVLLVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIILNPILFLPFIITPIVLVITSYTAISIGWVPKTVVMIPWATPPIISGYLVTGGHLSGAILQLFNF
VIAMVIYYPFVVLCDRSVVRTEKAAAQGNNNSVPM

Sequences:

>Translated_435_residues
MQKFIAFMEKYIVPVAGKIGSQRHLAAIRDGFIAVMPLILVGALASLINGFPSEAFQDFMKGLFGETWKQVGGGMWTGSF
AILALIIAFTTSYNLAKSYGVDGLSAGIISFGALIILTPTTPKEGGLNLAWTGAQGLFVAIIVALLVTEVFRFFVQRNIT
FKMPDGVPPAVLRSFAAIVPAFVILTVVAGIQLAVKLAGTSVHEFIFNTIQSPLQSLAGTLPSAIVIVLLVHLLWFFGLH
GPNIVGGIIEPLYLPALEKNMQLFKGGTSAFDVPNIVTKPFFDTFVYLGGSGATLAFLVVVLLVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIILNPILFLPFIITPIVLVITSYTAISIGWVPKTVVMIPWATPPIISGYLVTGGHLSGAILQLFNF
VIAMVIYYPFVVLCDRSVVRTEKAAAQGNNNSVPM
>Mature_435_residues
MQKFIAFMEKYIVPVAGKIGSQRHLAAIRDGFIAVMPLILVGALASLINGFPSEAFQDFMKGLFGETWKQVGGGMWTGSF
AILALIIAFTTSYNLAKSYGVDGLSAGIISFGALIILTPTTPKEGGLNLAWTGAQGLFVAIIVALLVTEVFRFFVQRNIT
FKMPDGVPPAVLRSFAAIVPAFVILTVVAGIQLAVKLAGTSVHEFIFNTIQSPLQSLAGTLPSAIVIVLLVHLLWFFGLH
GPNIVGGIIEPLYLPALEKNMQLFKGGTSAFDVPNIVTKPFFDTFVYLGGSGATLAFLVVVLLVAKSAQLRGVSRLSIGP
GAFNINEPVIFGTPIILNPILFLPFIITPIVLVITSYTAISIGWVPKTVVMIPWATPPIISGYLVTGGHLSGAILQLFNF
VIAMVIYYPFVVLCDRSVVRTEKAAAQGNNNSVPM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=451, Percent_Identity=35.920177383592, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: 2.7.1.69

Molecular weight: Translated: 46471; Mature: 46471

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFIAFMEKYIVPVAGKIGSQRHLAAIRDGFIAVMPLILVGALASLINGFPSEAFQDFM
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KGLFGETWKQVGGGMWTGSFAILALIIAFTTSYNLAKSYGVDGLSAGIISFGALIILTPT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCEEEEEECC
TPKEGGLNLAWTGAQGLFVAIIVALLVTEVFRFFVQRNITFKMPDGVPPAVLRSFAAIVP
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHH
AFVILTVVAGIQLAVKLAGTSVHEFIFNTIQSPLQSLAGTLPSAIVIVLLVHLLWFFGLH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPNIVGGIIEPLYLPALEKNMQLFKGGTSAFDVPNIVTKPFFDTFVYLGGSGATLAFLVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
VLLVAKSAQLRGVSRLSIGPGAFNINEPVIFGTPIILNPILFLPFIITPIVLVITSYTAI
HHHHHCCHHHCCCCEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHE
SIGWVPKTVVMIPWATPPIISGYLVTGGHLSGAILQLFNFVIAMVIYYPFVVLCDRSVVR
EECCCCCEEEEECCCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TEKAAAQGNNNSVPM
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MQKFIAFMEKYIVPVAGKIGSQRHLAAIRDGFIAVMPLILVGALASLINGFPSEAFQDFM
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KGLFGETWKQVGGGMWTGSFAILALIIAFTTSYNLAKSYGVDGLSAGIISFGALIILTPT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCEEEEEECC
TPKEGGLNLAWTGAQGLFVAIIVALLVTEVFRFFVQRNITFKMPDGVPPAVLRSFAAIVP
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHH
AFVILTVVAGIQLAVKLAGTSVHEFIFNTIQSPLQSLAGTLPSAIVIVLLVHLLWFFGLH
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPNIVGGIIEPLYLPALEKNMQLFKGGTSAFDVPNIVTKPFFDTFVYLGGSGATLAFLVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
VLLVAKSAQLRGVSRLSIGPGAFNINEPVIFGTPIILNPILFLPFIITPIVLVITSYTAI
HHHHHCCHHHCCCCEEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHE
SIGWVPKTVVMIPWATPPIISGYLVTGGHLSGAILQLFNFVIAMVIYYPFVVLCDRSVVR
EECCCCCEEEEECCCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TEKAAAQGNNNSVPM
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]