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The map label for this gene is ywkE [H]

Identifier: 52140234

GI number: 52140234

Start: 5125870

End: 5126721

Strand: Reverse

Name: ywkE [H]

Synonym: BCZK5028

Alternate gene names: 52140234

Gene position: 5126721-5125870 (Counterclockwise)

Preceding gene: 52140231

Following gene: 52140233

Centisome position: 96.71

GC content: 39.32

Gene sequence:

>852_bases
ATGCGTGTCTATGAAGCCCTGAAATGGGCTTCTTCTTTTTTACAGGAGAATGGGCGAGATGAAAATGCGGGAGAAATTGT
CCTTTGTCATGTATTAAAGACGAATAGAACAGGATTATTAATGAACATGCGTGAAGAAATAACTGTGGAACAAGAAACGA
GTTTTACAGAGTTTATCCACAAGCATGTAGAAGGTATTCCGATTCAATATATGATTGGTCATGAAATGTTTTATGGAAGA
TCGTTCTTTGTGAATGAAGAAGTATTAATACCAAGACCGGAAACAGAAGAGCTTATAGTGGGAGTGTTAGAAAGAATCGA
GCGCCATTTTGGTGATGAGAAACTACATGTAGCGGATATCGGTACGGGTAGCGGGGCGATTTCGATTACGCTTGCTTTAG
AAAATAAAAATCTTCATGTATATACGGTAGATATTGCACAGGAGTCAATTGAAGTTGCAAAAGAAAATGCGAAAACTTTG
GGCGCAGAAGTAACGTTCTATCATGGTGATTTATTATCACCATTTTATGAAACGGGTCAAAAGTTAGATGTTGTTGTTTC
AAATCCTCCATATATACCGGAAGAAGACTGGCGTGGTCTTTCTCCTGTGGTGAAAGAGCATGAGCCAAAGAGAGCGCTTG
TTGGTGGTGAAGACGGATTAGATTTCTATCGCCGCTTTATGGAAGAGTTGCCGAAGGTGTTGCAGAAGAAAGCAATTGTA
GCATTTGAAATTGGAGTAGGACAAGGTGAGGACGTGAAAGGGCTGTTACAAAAAGCTTTTCCACATGCTCATGTTGAGGT
TGTTTTTGATATTAATGGAAAAGATCGTATGGTATTTGCAGAAATGGAGTAA

Upstream 100 bases:

>100_bases
CGATTCAAAAGCTAGATCAAATCTTACAAGGTAAGTTAGATGATTTCATCAATGCCTTAGTGATGGAAGATCAGGCTCAA
AGGATGGAGGCAGCTGAGTA

Downstream 100 bases:

>100_bases
GGGTTACACCTTACTCTATTTTTTTATGGAAAAATTCATATAGATTTTGAATTTAATAGTTTAGAAAGATGAAAGTCTGT
CCACACTGTTTGTAGAAGGG

Product: HemK family modification methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITVEQETSFTEFIHKHVEGIPIQYMIGHEMFYGR
SFFVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADIGTGSGAISITLALENKNLHVYTVDIAQESIEVAKENAKTL
GAEVTFYHGDLLSPFYETGQKLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPKVLQKKAIV
AFEIGVGQGEDVKGLLQKAFPHAHVEVVFDINGKDRMVFAEME

Sequences:

>Translated_283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITVEQETSFTEFIHKHVEGIPIQYMIGHEMFYGR
SFFVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADIGTGSGAISITLALENKNLHVYTVDIAQESIEVAKENAKTL
GAEVTFYHGDLLSPFYETGQKLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPKVLQKKAIV
AFEIGVGQGEDVKGLLQKAFPHAHVEVVFDINGKDRMVFAEME
>Mature_283_residues
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITVEQETSFTEFIHKHVEGIPIQYMIGHEMFYGR
SFFVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADIGTGSGAISITLALENKNLHVYTVDIAQESIEVAKENAKTL
GAEVTFYHGDLLSPFYETGQKLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPKVLQKKAIV
AFEIGVGQGEDVKGLLQKAFPHAHVEVVFDINGKDRMVFAEME

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=270, Percent_Identity=29.2592592592593, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1787463, Length=278, Percent_Identity=29.8561151079137, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082085, Length=189, Percent_Identity=31.7460317460317, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324266, Length=232, Percent_Identity=29.3103448275862, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582226, Length=239, Percent_Identity=27.1966527196653, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32101; Mature: 32101

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITVEQETSFTEFIH
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEECHHHEEECCCCCHHHHHH
KHVEGIPIQYMIGHEMFYGRSFFVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADI
HHHCCCCEEEEECCEEECCCEEEECCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
GTGSGAISITLALENKNLHVYTVDIAQESIEVAKENAKTLGAEVTFYHGDLLSPFYETGQ
CCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCC
KLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPKVLQKKAIV
EEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEE
AFEIGVGQGEDVKGLLQKAFPHAHVEVVFDINGKDRMVFAEME
EEEECCCCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MRVYEALKWASSFLQENGRDENAGEIVLCHVLKTNRTGLLMNMREEITVEQETSFTEFIH
CCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEECHHHEEECCCCCHHHHHH
KHVEGIPIQYMIGHEMFYGRSFFVNEEVLIPRPETEELIVGVLERIERHFGDEKLHVADI
HHHCCCCEEEEECCEEECCCEEEECCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEE
GTGSGAISITLALENKNLHVYTVDIAQESIEVAKENAKTLGAEVTFYHGDLLSPFYETGQ
CCCCCEEEEEEEEECCCEEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCC
KLDVVVSNPPYIPEEDWRGLSPVVKEHEPKRALVGGEDGLDFYRRFMEELPKVLQKKAIV
EEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEE
AFEIGVGQGEDVKGLLQKAFPHAHVEVVFDINGKDRMVFAEME
EEEECCCCCCCHHHHHHHCCCCCEEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]