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The map label for this gene is ligA

Identifier: 51893963

GI number: 51893963

Start: 3040066

End: 3042087

Strand: Reverse

Name: ligA

Synonym: STH2825

Alternate gene names: 51893963

Gene position: 3042087-3040066 (Counterclockwise)

Preceding gene: 51893964

Following gene: 51893962

Centisome position: 85.3

GC content: 69.49

Gene sequence:

>2022_bases
ATGGATCGATCCTGGGCGGAGCGGCGGATCCCGGAACTGATCGAGACCATTCGCCACCATGAGTACCTCTATTACGTGAA
GAATGAGCCGGAGATCAGCGACGCCGCCTTCGACGAGCTGATGCAGGAGCTGAAGCAGCTGGAGGAGGCGTTCCCGGACC
TGCGGCGGCCCGACTCGCCGACGCAGCGGGTCGGCGGGGCCACCGCGCCCGACTTCGCCAAGGTACCCCACCAGCCGCCG
ATGTACTCCCTGGACAACGCCTTCAGCGAGGCGGATCTGCGGGACTTCGACCGGCGGGTGCGCGAGGGGCTGGGCGGCGA
GCCGGTCTCCTACGTGTGCGAGCTGAAGATCGACGGGCTCTCGATCTCGCTTCGCTACGAGGACGGCCTCTTCGTCCAGG
GCGCGACCCGGGGCGACGGCGAGACCGGCGAGGACGTCACCGAGAACCTGCGCACCATCGGCTCGATCCCGCTCCGGCTG
GACGGGACGGAGGCCCCGGTGCCGCCCCGGCTGATCGTGCGGGGCGAGGTCTACATGACCAAGCAGGTGCTGGAGGAGCT
GAATGCGGCGCTGGCCGCCGAGGGGAAGCCCCTGCTGCAGAACCCCCGTAACGCCGCGGCCGGCGGACTCCGGCAGAAGG
ATCCCCGCAAGACCCGGGAGCGGCGGCTGGACGCCTTCCTCTACCAGGTGGTGGACGCGGAGGCCCTGGGCATCGCCGAC
CACTGGAGTGCGCTGCAGCGGCTTCAGGCCTGGCGCTTCAAGGTGAACCCGCACCGGCACCTGGCCCACACCATCGACGA
GGTCCTGGACTGGGTGGCCGGGTGGCAGGCGCGGCGGCACGAGCTGCCCTATGAGATCGACGGCCTGGTGATCAAGGTCA
ACGACCTCGCCCAGCAGCGGCGGCTGGGCTTTACGTCCAAGTTCCCTCGCTGGGCGATCGCCTACAAGTTCCCCGCGGAG
GAGCGGGAGACCACCGTGGTGGGCATCAGCCTGGAGGTCGGGCGCACCGGCGTGGTGACGCCGTCGGCGGACCTGGCGCC
GGTGCGCATCGCCGGCACCACGGTGAAGCGCGCCACGCTGCACAACGAGGACTACATCCGGGAGAAGGACATCCGAGTCG
GGGACACGGTCATCGTCCGCAAGGCCGGCGAGATCATCCCCGAGGTGGTGCGGGTCGTGCCGGAGAAACGGCCGCCCGAC
GCCCAGCCCTGGACCTTCCCGAAGACGTGTCCCGCCTGCGGCGCAGAGCTGGTCCGGATCGAAGGCGAGGCGGCGACCCG
CTGCACCAACAACCTCTGCCCCGCCCAGCAGTACCGGGCGATCCTGCACTTCGCTTCGCGGGACGCCATGAACATCGAGG
GGCTGGGCGAGGCCCTGGTGCAGTCGCTGCTGGACCACGGCCTGATCGAGGACGCCGCCGACCTTTACCGGCTCCACGAG
AAGCGGGATGCGCTCATCCGCCTCGAGCGGATGGGCGAGAAGTCGGTGGACAACCTGCTGGCGGCCATCGACGCCACCCG
GCAGAACCCCCTGCACCGCCTGATCTTCGCCCTGGGCATCCGGCACGTCGGCGAGCGGGCAGCCCGGCTCCTGGCCGACC
ACTTCGGATCCATGGAGGCGATCGAGCAGGCCGGCCTCGACGAGCTCACGGCCATCCCCGGCCTGGGGCCGAAGATCGCG
GAGTCGGTGAAGAACTACTTCGCCTCGCCCCGCTCGCATCAGCTGCTGGCGAAGCTCAGGGCCGCGGGCGTGAACATGGT
GGGCGAGAAGAAGGCTGGGCCGGCAGAGGGGCCGCTGGCGGGCATGACCGTGGTGGTCACCGGGACGCTGGTGCGGTGGA
GCCGGAAGGAGATCGAGGAACTGATCCAGCAGTTGGGCGGGAAGGCTGCCGGTTCGGTCAGCCGGAAGACCAGCTTCGTC
GTTGCGGGGGAGGCCGCGGGCTCCAAGCTGCAGAAGGCTCAGGAACTGGGCATCCCGGTGCTCACCGAAGACGAGTTCTG
CGAGCGCTATCTCCAGGGCTAG

Upstream 100 bases:

>100_bases
TGACCGTGCAGTTCGCCGGGGCGGCCGGGCAGCGGGTACTGGTGGCCAGCTACCTGCGCCCGGCCGCGGCCGAGGCGGAG
TGACTGGGGAGGGAAGCAGC

Downstream 100 bases:

>100_bases
GGAAACCTCGCCAAGATGATGCAGGGGGAATCGGGAGGATTCGCGTATAGGCTGAGCGAAAAAATCTAAACCAACCCGCG
CGAAACTGCGTCTCATGAGC

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MDRSWAERRIPELIETIRHHEYLYYVKNEPEISDAAFDELMQELKQLEEAFPDLRRPDSPTQRVGGATAPDFAKVPHQPP
MYSLDNAFSEADLRDFDRRVREGLGGEPVSYVCELKIDGLSISLRYEDGLFVQGATRGDGETGEDVTENLRTIGSIPLRL
DGTEAPVPPRLIVRGEVYMTKQVLEELNAALAAEGKPLLQNPRNAAAGGLRQKDPRKTRERRLDAFLYQVVDAEALGIAD
HWSALQRLQAWRFKVNPHRHLAHTIDEVLDWVAGWQARRHELPYEIDGLVIKVNDLAQQRRLGFTSKFPRWAIAYKFPAE
ERETTVVGISLEVGRTGVVTPSADLAPVRIAGTTVKRATLHNEDYIREKDIRVGDTVIVRKAGEIIPEVVRVVPEKRPPD
AQPWTFPKTCPACGAELVRIEGEAATRCTNNLCPAQQYRAILHFASRDAMNIEGLGEALVQSLLDHGLIEDAADLYRLHE
KRDALIRLERMGEKSVDNLLAAIDATRQNPLHRLIFALGIRHVGERAARLLADHFGSMEAIEQAGLDELTAIPGLGPKIA
ESVKNYFASPRSHQLLAKLRAAGVNMVGEKKAGPAEGPLAGMTVVVTGTLVRWSRKEIEELIQQLGGKAAGSVSRKTSFV
VAGEAAGSKLQKAQELGIPVLTEDEFCERYLQG

Sequences:

>Translated_673_residues
MDRSWAERRIPELIETIRHHEYLYYVKNEPEISDAAFDELMQELKQLEEAFPDLRRPDSPTQRVGGATAPDFAKVPHQPP
MYSLDNAFSEADLRDFDRRVREGLGGEPVSYVCELKIDGLSISLRYEDGLFVQGATRGDGETGEDVTENLRTIGSIPLRL
DGTEAPVPPRLIVRGEVYMTKQVLEELNAALAAEGKPLLQNPRNAAAGGLRQKDPRKTRERRLDAFLYQVVDAEALGIAD
HWSALQRLQAWRFKVNPHRHLAHTIDEVLDWVAGWQARRHELPYEIDGLVIKVNDLAQQRRLGFTSKFPRWAIAYKFPAE
ERETTVVGISLEVGRTGVVTPSADLAPVRIAGTTVKRATLHNEDYIREKDIRVGDTVIVRKAGEIIPEVVRVVPEKRPPD
AQPWTFPKTCPACGAELVRIEGEAATRCTNNLCPAQQYRAILHFASRDAMNIEGLGEALVQSLLDHGLIEDAADLYRLHE
KRDALIRLERMGEKSVDNLLAAIDATRQNPLHRLIFALGIRHVGERAARLLADHFGSMEAIEQAGLDELTAIPGLGPKIA
ESVKNYFASPRSHQLLAKLRAAGVNMVGEKKAGPAEGPLAGMTVVVTGTLVRWSRKEIEELIQQLGGKAAGSVSRKTSFV
VAGEAAGSKLQKAQELGIPVLTEDEFCERYLQG
>Mature_673_residues
MDRSWAERRIPELIETIRHHEYLYYVKNEPEISDAAFDELMQELKQLEEAFPDLRRPDSPTQRVGGATAPDFAKVPHQPP
MYSLDNAFSEADLRDFDRRVREGLGGEPVSYVCELKIDGLSISLRYEDGLFVQGATRGDGETGEDVTENLRTIGSIPLRL
DGTEAPVPPRLIVRGEVYMTKQVLEELNAALAAEGKPLLQNPRNAAAGGLRQKDPRKTRERRLDAFLYQVVDAEALGIAD
HWSALQRLQAWRFKVNPHRHLAHTIDEVLDWVAGWQARRHELPYEIDGLVIKVNDLAQQRRLGFTSKFPRWAIAYKFPAE
ERETTVVGISLEVGRTGVVTPSADLAPVRIAGTTVKRATLHNEDYIREKDIRVGDTVIVRKAGEIIPEVVRVVPEKRPPD
AQPWTFPKTCPACGAELVRIEGEAATRCTNNLCPAQQYRAILHFASRDAMNIEGLGEALVQSLLDHGLIEDAADLYRLHE
KRDALIRLERMGEKSVDNLLAAIDATRQNPLHRLIFALGIRHVGERAARLLADHFGSMEAIEQAGLDELTAIPGLGPKIA
ESVKNYFASPRSHQLLAKLRAAGVNMVGEKKAGPAEGPLAGMTVVVTGTLVRWSRKEIEELIQQLGGKAAGSVSRKTSFV
VAGEAAGSKLQKAQELGIPVLTEDEFCERYLQG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=665, Percent_Identity=45.8646616541353, Blast_Score=583, Evalue=1e-167,
Organism=Escherichia coli, GI87082305, Length=527, Percent_Identity=23.1499051233397, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SYMTH (Q67KI8)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076654.1
- HSSP:   O87703
- ProteinModelPortal:   Q67KI8
- GeneID:   2981563
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2825
- NMPDR:   fig|292459.1.peg.2700
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- BioCyc:   STHE292459:STH2825-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74735; Mature: 74735

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 116-116 BINDING 114-114 BINDING 137-137 BINDING 176-176 BINDING 292-292 BINDING 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSWAERRIPELIETIRHHEYLYYVKNEPEISDAAFDELMQELKQLEEAFPDLRRPDSP
CCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TQRVGGATAPDFAKVPHQPPMYSLDNAFSEADLRDFDRRVREGLGGEPVSYVCELKIDGL
HHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCE
SISLRYEDGLFVQGATRGDGETGEDVTENLRTIGSIPLRLDGTEAPVPPRLIVRGEVYMT
EEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECHHHHH
KQVLEELNAALAAEGKPLLQNPRNAAAGGLRQKDPRKTRERRLDAFLYQVVDAEALGIAD
HHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
HWSALQRLQAWRFKVNPHRHLAHTIDEVLDWVAGWQARRHELPYEIDGLVIKVNDLAQQR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEHHHHHHH
RLGFTSKFPRWAIAYKFPAEERETTVVGISLEVGRTGVVTPSADLAPVRIAGTTVKRATL
HCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHH
HNEDYIREKDIRVGDTVIVRKAGEIIPEVVRVVPEKRPPDAQPWTFPKTCPACGAELVRI
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCEEEEE
EGEAATRCTNNLCPAQQYRAILHFASRDAMNIEGLGEALVQSLLDHGLIEDAADLYRLHE
CCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KRDALIRLERMGEKSVDNLLAAIDATRQNPLHRLIFALGIRHVGERAARLLADHFGSMEA
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IEQAGLDELTAIPGLGPKIAESVKNYFASPRSHQLLAKLRAAGVNMVGEKKAGPAEGPLA
HHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GMTVVVTGTLVRWSRKEIEELIQQLGGKAAGSVSRKTSFVVAGEAAGSKLQKAQELGIPV
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCE
LTEDEFCERYLQG
ECCHHHHHHHHCC
>Mature Secondary Structure
MDRSWAERRIPELIETIRHHEYLYYVKNEPEISDAAFDELMQELKQLEEAFPDLRRPDSP
CCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
TQRVGGATAPDFAKVPHQPPMYSLDNAFSEADLRDFDRRVREGLGGEPVSYVCELKIDGL
HHHCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCE
SISLRYEDGLFVQGATRGDGETGEDVTENLRTIGSIPLRLDGTEAPVPPRLIVRGEVYMT
EEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECHHHHH
KQVLEELNAALAAEGKPLLQNPRNAAAGGLRQKDPRKTRERRLDAFLYQVVDAEALGIAD
HHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
HWSALQRLQAWRFKVNPHRHLAHTIDEVLDWVAGWQARRHELPYEIDGLVIKVNDLAQQR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEHHHHHHH
RLGFTSKFPRWAIAYKFPAEERETTVVGISLEVGRTGVVTPSADLAPVRIAGTTVKRATL
HCCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEECCHHHHHHH
HNEDYIREKDIRVGDTVIVRKAGEIIPEVVRVVPEKRPPDAQPWTFPKTCPACGAELVRI
CCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCEEEEE
EGEAATRCTNNLCPAQQYRAILHFASRDAMNIEGLGEALVQSLLDHGLIEDAADLYRLHE
CCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
KRDALIRLERMGEKSVDNLLAAIDATRQNPLHRLIFALGIRHVGERAARLLADHFGSMEA
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IEQAGLDELTAIPGLGPKIAESVKNYFASPRSHQLLAKLRAAGVNMVGEKKAGPAEGPLA
HHHCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
GMTVVVTGTLVRWSRKEIEELIQQLGGKAAGSVSRKTSFVVAGEAAGSKLQKAQELGIPV
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCE
LTEDEFCERYLQG
ECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA