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The map label for this gene is nuoB2

Identifier: 51893915

GI number: 51893915

Start: 2996137

End: 2996685

Strand: Reverse

Name: nuoB2

Synonym: STH2777

Alternate gene names: 51893915

Gene position: 2996685-2996137 (Counterclockwise)

Preceding gene: 51893916

Following gene: 51893914

Centisome position: 84.03

GC content: 62.3

Gene sequence:

>549_bases
TTGAGCCAGGCGCGCCACGAGCTGGATTGGCTCAACGACGAGGTAGTCGTCTCGGAAATTGACGTCGTCAACGAGAACGT
CATTGCCGGCAAACTGCCCGACGTCATCAAGAAGGTGTTGGGGTGGGGCTACGGCAACTCGACCTGGCCCCTGGGCTTCG
GCATCGCGTGCTGCGCCATCGAGATGATGGCGGCCTGCAGTTCGAAGTACGATATCGCCCGCTTCGGCTCCGAGGCGATG
CGCATGTCGCCGCGCCAGGCCGACGTGATGATCGTGGCCGGCACGGTGAACGAGAAGATGGCAGTCGTGGTTCGGCGGCT
CTACGACCAGATGTCGGAGCCTAAATTTGTGATTGCCATGGGTGCGTGCGCATCCAACGGCGGCCCTTACTGGGACACGT
ACAACGTGGTCGATGGCGTTCACAAGGTCGTGCCGGTCGACGTGTACGTGCCGGGGTGCCCGCCCCGGCCTGAGGCATTG
CTCCAGGGACTCATGGAGATCCAGGAGATGATCAAGACCGGCAAGCGCGGCCTGATCACGAAGGGTTAG

Upstream 100 bases:

>100_bases
TAAAGAAACATGACAGTATAATGGGCAGTAGTGTGTGACGGAAGGTGCATGGGGTCGCCTAGTACGAACTGACTCTTCCC
AGAACAGGGGAGGTGACACC

Downstream 100 bases:

>100_bases
CATCATCTGGGCAGGGCCGGTTGCCCTGCCCTTTGACCGGAGGCCGGGTCACTGCGGCCTCCGACGTCGCTCTCGGAAAG
GGGATAATGCCCGGTGAGCT

Product: NADH dehydrogenase I subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 2; NDH-1 subunit B 2

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MSQARHELDWLNDEVVVSEIDVVNENVIAGKLPDVIKKVLGWGYGNSTWPLGFGIACCAIEMMAACSSKYDIARFGSEAM
RMSPRQADVMIVAGTVNEKMAVVVRRLYDQMSEPKFVIAMGACASNGGPYWDTYNVVDGVHKVVPVDVYVPGCPPRPEAL
LQGLMEIQEMIKTGKRGLITKG

Sequences:

>Translated_182_residues
MSQARHELDWLNDEVVVSEIDVVNENVIAGKLPDVIKKVLGWGYGNSTWPLGFGIACCAIEMMAACSSKYDIARFGSEAM
RMSPRQADVMIVAGTVNEKMAVVVRRLYDQMSEPKFVIAMGACASNGGPYWDTYNVVDGVHKVVPVDVYVPGCPPRPEAL
LQGLMEIQEMIKTGKRGLITKG
>Mature_181_residues
SQARHELDWLNDEVVVSEIDVVNENVIAGKLPDVIKKVLGWGYGNSTWPLGFGIACCAIEMMAACSSKYDIARFGSEAMR
MSPRQADVMIVAGTVNEKMAVVVRRLYDQMSEPKFVIAMGACASNGGPYWDTYNVVDGVHKVVPVDVYVPGCPPRPEALL
QGLMEIQEMIKTGKRGLITKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=152, Percent_Identity=51.3157894736842, Blast_Score=166, Evalue=1e-41,
Organism=Escherichia coli, GI1788624, Length=156, Percent_Identity=57.6923076923077, Blast_Score=190, Evalue=6e-50,
Organism=Escherichia coli, GI1789074, Length=111, Percent_Identity=42.3423423423423, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1788834, Length=111, Percent_Identity=37.8378378378378, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=140, Percent_Identity=52.1428571428571, Blast_Score=156, Evalue=4e-39,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=52.1428571428571, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=52.1428571428571, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=53.2846715328467, Blast_Score=162, Evalue=1e-40,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB2_SYMTH (Q67KN6)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076606.1
- ProteinModelPortal:   Q67KN6
- SMR:   Q67KN6
- GeneID:   2979670
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2777
- NMPDR:   fig|292459.1.peg.2654
- HOGENOM:   HBG553221
- OMA:   EAKWVIS
- BioCyc:   STHE292459:STH2777-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 19911; Mature: 19780

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQARHELDWLNDEVVVSEIDVVNENVIAGKLPDVIKKVLGWGYGNSTWPLGFGIACCAI
CCCHHHHHHHCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EMMAACSSKYDIARFGSEAMRMSPRQADVMIVAGTVNEKMAVVVRRLYDQMSEPKFVIAM
HHHHHHCCCCHHHHHCHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEE
GACASNGGPYWDTYNVVDGVHKVVPVDVYVPGCPPRPEALLQGLMEIQEMIKTGKRGLIT
ECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
KG
CC
>Mature Secondary Structure 
SQARHELDWLNDEVVVSEIDVVNENVIAGKLPDVIKKVLGWGYGNSTWPLGFGIACCAI
CCHHHHHHHCCCCEEHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EMMAACSSKYDIARFGSEAMRMSPRQADVMIVAGTVNEKMAVVVRRLYDQMSEPKFVIAM
HHHHHHCCCCHHHHHCHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEE
GACASNGGPYWDTYNVVDGVHKVVPVDVYVPGCPPRPEALLQGLMEIQEMIKTGKRGLIT
ECCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
KG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA