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The map label for this gene is appA [H]

Identifier: 51893883

GI number: 51893883

Start: 2958996

End: 2960651

Strand: Reverse

Name: appA [H]

Synonym: STH2745

Alternate gene names: 51893883

Gene position: 2960651-2958996 (Counterclockwise)

Preceding gene: 51893885

Following gene: 51893882

Centisome position: 83.02

GC content: 71.32

Gene sequence:

>1656_bases
ATGGCATCCTCCGACGAGATGCCTGCAGACGGCGGCACGCTGTACCTCTCCATGTTCTCCGCGCCCCGGGGCGTCTTCAA
CCCCATCCTGATGGAGGACGGCTACGACGCGACCATCGTCGGCCTGGTCTTCGCCGGACTGCTGACCCGGGACGAGAACC
TGGACCTGGTCTGCGACCTGTGCGAAAGCTTTGCTGTGTCCCCGGATCACCGGCAGTTCACCTTCCGCCTGCGTCGGGAC
GTCACCTGGCACGACGGCCACCCCTTCACGGCGGCCGATGTGGCATTCACCTTCCGAACCATTCTCCACCCCGACTACCC
CGGGGTGCTGACCGGCGAGTACGCCGCCCTGCGCGGCGTCGCGGCCATGCTGGACGACCGGGCGGCCGTGGACCGGGAGC
TGGCCGCCGGCCGGATCACGGCGGACGAGGCGGCGGCGCGGAAGAGGGCGTCCTGGCAGGCCTGGCTGGACGGGGAGGGG
CGGGAGGCGATCCGGGTGGCGGACGATCACACCATCGTCTTCGCGGCGGACCGGCCCCATGCCCCGCTGCTGCAGAACCT
GGCCATCCCGCTGCTTCCCGCCCACGTCTTCGCCGGGACGCCGCCGGGCGACCTCGGCGACCACCCGGCCACGCGGATGC
CGGTGGGCACCGGACCGTACCAGTTCGTGCGCTACGTGCCGGACCAGTACGTGGAGCTGGTGCGCCACGAGACCTACCAC
CGCGGCCGGCCCCACATCGAGCGGATCATCTACCGGATGGTCAACCAGGACGTGGCCATCGGACAGCTGAAGGCGGGCGA
GCTGGACTACGTCGTGCTGAAGCCCGGGGATGTGGCCCTGGTGGCGGACGACCCGGACATCGGGATCTTCGTGCGACCCG
ATCTGGGGTACCAGTACATGGGGCTCAACCACCGCAACCCGCTCTTTGCCGACCGGCGGGTGCGCGAGGCCATCCTGCTG
GCCATCGACCGGCAGGCGATCGTCGACCACCTGCTGAAGGGGTACGGCCACGTCGCCGAGGGCCACCTCCCGTACGGCCA
CCCGTACCACGATCTGGAAGCGACGCCGGTGCGCCGTCGAGACACGGTCGCGGCGGCCCGTCTCCTGGCCGAGGCCGGCT
GGACGGAGCGGGACGCGGAGGGCTACCTGGTCCGGGATGGCCGTCGCTTCCGCTTCACCCTGCTCTACCCCTCCGGCAAC
GCCGTACGGGCGGCCACGGCCCCGCTCATACAGGCCGATCTGAAGGCGGTGGGCATCCAGGTTGATCTTCAGATGATGGA
GTTCTCCACCCTCGCGGCGACGGTCTTCGGCGGCGAGCGGCCGGACGCCTGGCTGATGGGCTGGGGCGTGGGGCTCGATC
CGGATCCGGGGCCGGTGTTCGACCCGGACAGCCGGTGGGGGCAGGCGACGGGCTGGCGTAGCGCCCGCAGCCGGATGCTG
CTGGGCATGGGGGCGGCCCGGCTGGAGCCCGAGGCGCGGCGACCGGTGTACCGGGCGTGGTCCCGCCTGCTGGCCGATGA
GCTGCCCGTGATCTTCCTGTACAGCCAGGACCAGATCGAGGCGGTGCGGCTGGACCGGGTGCGGGGGCTGCGACCGGACG
CCCGGGGCGCCCTCTGGAACATCTGGGAGCTCTGGATCGCGTCCGGGCGCCGCTGA

Upstream 100 bases:

>100_bases
ATGCGCCACACCAGCTTACCCCTGGGCGGGCATCCGCGGTGCCGCGGGCGCCGCAGCCGGGGGTCCATCGTCCTCCGGCG
GGGCGCAGACAGCCGAACCG

Downstream 100 bases:

>100_bases
GGCCCACGACGGTCGTACAGCTGGGCTTCCCGGACGGCGGCCGGGTGTGAGGGAGGGACGGGGCGGTGGCTGGATTCATC
CTGCGGCGGCTCCTGCAACT

Product: oligopeptide ABC transporter substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MASSDEMPADGGTLYLSMFSAPRGVFNPILMEDGYDATIVGLVFAGLLTRDENLDLVCDLCESFAVSPDHRQFTFRLRRD
VTWHDGHPFTAADVAFTFRTILHPDYPGVLTGEYAALRGVAAMLDDRAAVDRELAAGRITADEAAARKRASWQAWLDGEG
REAIRVADDHTIVFAADRPHAPLLQNLAIPLLPAHVFAGTPPGDLGDHPATRMPVGTGPYQFVRYVPDQYVELVRHETYH
RGRPHIERIIYRMVNQDVAIGQLKAGELDYVVLKPGDVALVADDPDIGIFVRPDLGYQYMGLNHRNPLFADRRVREAILL
AIDRQAIVDHLLKGYGHVAEGHLPYGHPYHDLEATPVRRRDTVAAARLLAEAGWTERDAEGYLVRDGRRFRFTLLYPSGN
AVRAATAPLIQADLKAVGIQVDLQMMEFSTLAATVFGGERPDAWLMGWGVGLDPDPGPVFDPDSRWGQATGWRSARSRML
LGMGAARLEPEARRPVYRAWSRLLADELPVIFLYSQDQIEAVRLDRVRGLRPDARGALWNIWELWIASGRR

Sequences:

>Translated_551_residues
MASSDEMPADGGTLYLSMFSAPRGVFNPILMEDGYDATIVGLVFAGLLTRDENLDLVCDLCESFAVSPDHRQFTFRLRRD
VTWHDGHPFTAADVAFTFRTILHPDYPGVLTGEYAALRGVAAMLDDRAAVDRELAAGRITADEAAARKRASWQAWLDGEG
REAIRVADDHTIVFAADRPHAPLLQNLAIPLLPAHVFAGTPPGDLGDHPATRMPVGTGPYQFVRYVPDQYVELVRHETYH
RGRPHIERIIYRMVNQDVAIGQLKAGELDYVVLKPGDVALVADDPDIGIFVRPDLGYQYMGLNHRNPLFADRRVREAILL
AIDRQAIVDHLLKGYGHVAEGHLPYGHPYHDLEATPVRRRDTVAAARLLAEAGWTERDAEGYLVRDGRRFRFTLLYPSGN
AVRAATAPLIQADLKAVGIQVDLQMMEFSTLAATVFGGERPDAWLMGWGVGLDPDPGPVFDPDSRWGQATGWRSARSRML
LGMGAARLEPEARRPVYRAWSRLLADELPVIFLYSQDQIEAVRLDRVRGLRPDARGALWNIWELWIASGRR
>Mature_550_residues
ASSDEMPADGGTLYLSMFSAPRGVFNPILMEDGYDATIVGLVFAGLLTRDENLDLVCDLCESFAVSPDHRQFTFRLRRDV
TWHDGHPFTAADVAFTFRTILHPDYPGVLTGEYAALRGVAAMLDDRAAVDRELAAGRITADEAAARKRASWQAWLDGEGR
EAIRVADDHTIVFAADRPHAPLLQNLAIPLLPAHVFAGTPPGDLGDHPATRMPVGTGPYQFVRYVPDQYVELVRHETYHR
GRPHIERIIYRMVNQDVAIGQLKAGELDYVVLKPGDVALVADDPDIGIFVRPDLGYQYMGLNHRNPLFADRRVREAILLA
IDRQAIVDHLLKGYGHVAEGHLPYGHPYHDLEATPVRRRDTVAAARLLAEAGWTERDAEGYLVRDGRRFRFTLLYPSGNA
VRAATAPLIQADLKAVGIQVDLQMMEFSTLAATVFGGERPDAWLMGWGVGLDPDPGPVFDPDSRWGQATGWRSARSRMLL
GMGAARLEPEARRPVYRAWSRLLADELPVIFLYSQDQIEAVRLDRVRGLRPDARGALWNIWELWIASGRR

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=558, Percent_Identity=24.7311827956989, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1787551, Length=550, Percent_Identity=24.3636363636364, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789887, Length=423, Percent_Identity=24.3498817966903, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787052, Length=440, Percent_Identity=23.6363636363636, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789397, Length=514, Percent_Identity=22.568093385214, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787762, Length=478, Percent_Identity=20.9205020920502, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61404; Mature: 61272

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSDEMPADGGTLYLSMFSAPRGVFNPILMEDGYDATIVGLVFAGLLTRDENLDLVCDL
CCCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHH
CESFAVSPDHRQFTFRLRRDVTWHDGHPFTAADVAFTFRTILHPDYPGVLTGEYAALRGV
HHHHCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AAMLDDRAAVDRELAAGRITADEAAARKRASWQAWLDGEGREAIRVADDHTIVFAADRPH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCEEEEECCCEEEEECCCCC
APLLQNLAIPLLPAHVFAGTPPGDLGDHPATRMPVGTGPYQFVRYVPDQYVELVRHETYH
CHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHC
RGRPHIERIIYRMVNQDVAIGQLKAGELDYVVLKPGDVALVADDPDIGIFVRPDLGYQYM
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCEEEEECCCCEEEEEECCCCEEEE
GLNHRNPLFADRRVREAILLAIDRQAIVDHLLKGYGHVAEGHLPYGHPYHDLEATPVRRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
DTVAAARLLAEAGWTERDAEGYLVRDGRRFRFTLLYPSGNAVRAATAPLIQADLKAVGIQ
HHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCEEEHHHHHHHHHHHHHEEEE
VDLQMMEFSTLAATVFGGERPDAWLMGWGVGLDPDPGPVFDPDSRWGQATGWRSARSRML
EEEEHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEE
LGMGAARLEPEARRPVYRAWSRLLADELPVIFLYSQDQIEAVRLDRVRGLRPDARGALWN
EECCHHHCCCHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCHHHH
IWELWIASGRR
HHHHHHHCCCC
>Mature Secondary Structure 
ASSDEMPADGGTLYLSMFSAPRGVFNPILMEDGYDATIVGLVFAGLLTRDENLDLVCDL
CCCCCCCCCCCEEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHH
CESFAVSPDHRQFTFRLRRDVTWHDGHPFTAADVAFTFRTILHPDYPGVLTGEYAALRGV
HHHHCCCCCCCEEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
AAMLDDRAAVDRELAAGRITADEAAARKRASWQAWLDGEGREAIRVADDHTIVFAADRPH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCEEEEECCCEEEEECCCCC
APLLQNLAIPLLPAHVFAGTPPGDLGDHPATRMPVGTGPYQFVRYVPDQYVELVRHETYH
CHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHC
RGRPHIERIIYRMVNQDVAIGQLKAGELDYVVLKPGDVALVADDPDIGIFVRPDLGYQYM
CCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCEEEEECCCCEEEEEECCCCEEEE
GLNHRNPLFADRRVREAILLAIDRQAIVDHLLKGYGHVAEGHLPYGHPYHDLEATPVRRR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHH
DTVAAARLLAEAGWTERDAEGYLVRDGRRFRFTLLYPSGNAVRAATAPLIQADLKAVGIQ
HHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEEEECCCCEEEHHHHHHHHHHHHHEEEE
VDLQMMEFSTLAATVFGGERPDAWLMGWGVGLDPDPGPVFDPDSRWGQATGWRSARSRML
EEEEHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHEE
LGMGAARLEPEARRPVYRAWSRLLADELPVIFLYSQDQIEAVRLDRVRGLRPDARGALWN
EECCHHHCCCHHCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCCHHHH
IWELWIASGRR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]