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The map label for this gene is murE [H]

Identifier: 51893869

GI number: 51893869

Start: 2932771

End: 2934261

Strand: Reverse

Name: murE [H]

Synonym: STH2731

Alternate gene names: 51893869

Gene position: 2934261-2932771 (Counterclockwise)

Preceding gene: 51893870

Following gene: 51893868

Centisome position: 82.28

GC content: 72.1

Gene sequence:

>1491_bases
TTGCTGTCGGCCCTGGACCCCGTGCAGGTGCTGGAGGCTCCCGACGTCACCGTGACCGGCATCGCCGCCGATTCCCGCGC
GGTGAGGCCCGGCGACCTGTTCGTGTGCATTCCGGGCGCGCACGTGGATGCGCACCAGTTCATCCCGGATGCCCTCCGGC
GCGGGGCGGTGGCGGTAGTCACGCAGCGCCCCTGGGCGGTTCCGACCGGCATCGGCGCGGCGGTGGTGCCCGACGCCCGG
CTGGCCCTCAGCGCCCTGGCCGACCGCTTCTACGGACATCCCTCCCGCGCGCTCCGGATGGTGGGGGTCACGGGAACCAA
CGGCAAGACCACGACGGCCTTCCTGGTGGAGGCCATCGCCCGCACGGCCGGCAAGGCGATGGGGGTGATCGGCACGGCAG
GCATGATGCTGGGCCGGGAGGTGCTGGAGGGCAAGTCGGGGTACACCACGCCCGAGCCCCAGACGATTCACCGGCTGCTG
GCTGCGATGCTGCAGCGCGGGGCCTGGGGCGCGGTGATGGAGGTCTCCAGCCACGCGCTGGAGCAGCGGCGCACGGACCA
CTGCCGGTTCGACGTCGCCGTCTTCACCAACCTCACCCACGAGCACCTGGATTACCACGGCGACATGGAGGGCTACTACG
CCGCCAAGGCCCGGCTCTTCCGCGGGCTGCAGCCGGGCGCGGCCGCGGTGGTGAACATCGACGACCCCTACGGCCGCCGG
TTGGCGGAGGAGATCCCCGCCGGAGTGCGCACCCTTACATACGGCTTCAGTGAGGGGGCGGATATCCGGGCCGAGGACGT
GCACCTCACCGGCCCGGGCGCCCGCTACCGGCTCATCACGCCGGTGGGCGAGGCCGACGTGGAGGCCCCGCACCTCTTCG
GCACCTACAATGTGTCCAACGCCATGGCCGCGGTGGGGGCCGGTCTGGCCCTGGGCTTCTCGCTGCAGGACGCCGTCGCG
GCGCTCGGCACGGCCAAGGGCGCGCCGGGCCGGTTCGAGCGCATCGACGAAGGACAGGACTTCACGGTGGTGGTGGATTA
CGCCCACACGCCGGACGGCTTCGAGAAGCTGCTCTCCGACGTGGCCCGGATCAAGGAGCCGGGCTCCCGGGTGATCATGG
TCTTCGGTTCGGCCGGCCAGCGGGATCCGTCGAAGCGGCCCGACATGGGCCGGATCGCCGGCGAGTACTGCGACGTGCTG
GTCCTGACGGAGGAGGATCCCCGCACCGAGGACGCGATGGAGATCGCGCGGCAGATCGCCTCGGGCGTGGCCCGGGACGA
GGTGGAGATCCACCTGATCGAGGACCGGGTGGAGGCCATCCGGGAGGCGATCCGCATGGCCCGCACCGGCGACATTGTGC
TCATCACCGGCAAGGGGCAGGAGACGGAGCTGGAGGTGCAGCACCCGACCACCTGGCGGGGCGACGTCGTGGAGGCGGTG
GACGCGCTGCGGGACCGCGCCCGGGAGGAGGCCCGGGGCGTGCGGGCGTAG

Upstream 100 bases:

>100_bases
GTCGGCCGTGCCCGACAGGATGCGCGGGCCGCACGTCGAAAGACGCTTAGATAATCAGGGCTTTAATGTGCAGGAGGGAC
TACCGTTGAAGTTGGTCGAT

Downstream 100 bases:

>100_bases
CAGAGCCGCCCGCCGCGTTCAGGGGTTGCCCGGCCGTGGAACGTCCCACGGGTGCATGGCCTGGAGGATGGTTCAGGGCA
TCGTACCTAGGTACGATGCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MLSALDPVQVLEAPDVTVTGIAADSRAVRPGDLFVCIPGAHVDAHQFIPDALRRGAVAVVTQRPWAVPTGIGAAVVPDAR
LALSALADRFYGHPSRALRMVGVTGTNGKTTTAFLVEAIARTAGKAMGVIGTAGMMLGREVLEGKSGYTTPEPQTIHRLL
AAMLQRGAWGAVMEVSSHALEQRRTDHCRFDVAVFTNLTHEHLDYHGDMEGYYAAKARLFRGLQPGAAAVVNIDDPYGRR
LAEEIPAGVRTLTYGFSEGADIRAEDVHLTGPGARYRLITPVGEADVEAPHLFGTYNVSNAMAAVGAGLALGFSLQDAVA
ALGTAKGAPGRFERIDEGQDFTVVVDYAHTPDGFEKLLSDVARIKEPGSRVIMVFGSAGQRDPSKRPDMGRIAGEYCDVL
VLTEEDPRTEDAMEIARQIASGVARDEVEIHLIEDRVEAIREAIRMARTGDIVLITGKGQETELEVQHPTTWRGDVVEAV
DALRDRAREEARGVRA

Sequences:

>Translated_496_residues
MLSALDPVQVLEAPDVTVTGIAADSRAVRPGDLFVCIPGAHVDAHQFIPDALRRGAVAVVTQRPWAVPTGIGAAVVPDAR
LALSALADRFYGHPSRALRMVGVTGTNGKTTTAFLVEAIARTAGKAMGVIGTAGMMLGREVLEGKSGYTTPEPQTIHRLL
AAMLQRGAWGAVMEVSSHALEQRRTDHCRFDVAVFTNLTHEHLDYHGDMEGYYAAKARLFRGLQPGAAAVVNIDDPYGRR
LAEEIPAGVRTLTYGFSEGADIRAEDVHLTGPGARYRLITPVGEADVEAPHLFGTYNVSNAMAAVGAGLALGFSLQDAVA
ALGTAKGAPGRFERIDEGQDFTVVVDYAHTPDGFEKLLSDVARIKEPGSRVIMVFGSAGQRDPSKRPDMGRIAGEYCDVL
VLTEEDPRTEDAMEIARQIASGVARDEVEIHLIEDRVEAIREAIRMARTGDIVLITGKGQETELEVQHPTTWRGDVVEAV
DALRDRAREEARGVRA
>Mature_496_residues
MLSALDPVQVLEAPDVTVTGIAADSRAVRPGDLFVCIPGAHVDAHQFIPDALRRGAVAVVTQRPWAVPTGIGAAVVPDAR
LALSALADRFYGHPSRALRMVGVTGTNGKTTTAFLVEAIARTAGKAMGVIGTAGMMLGREVLEGKSGYTTPEPQTIHRLL
AAMLQRGAWGAVMEVSSHALEQRRTDHCRFDVAVFTNLTHEHLDYHGDMEGYYAAKARLFRGLQPGAAAVVNIDDPYGRR
LAEEIPAGVRTLTYGFSEGADIRAEDVHLTGPGARYRLITPVGEADVEAPHLFGTYNVSNAMAAVGAGLALGFSLQDAVA
ALGTAKGAPGRFERIDEGQDFTVVVDYAHTPDGFEKLLSDVARIKEPGSRVIMVFGSAGQRDPSKRPDMGRIAGEYCDVL
VLTEEDPRTEDAMEIARQIASGVARDEVEIHLIEDRVEAIREAIRMARTGDIVLITGKGQETELEVQHPTTWRGDVVEAV
DALRDRAREEARGVRA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=463, Percent_Identity=38.8768898488121, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI1786274, Length=373, Percent_Identity=29.2225201072386, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786279, Length=202, Percent_Identity=31.6831683168317, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53239; Mature: 53239

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSALDPVQVLEAPDVTVTGIAADSRAVRPGDLFVCIPGAHVDAHQFIPDALRRGAVAVV
CCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEEE
TQRPWAVPTGIGAAVVPDARLALSALADRFYGHPSRALRMVGVTGTNGKTTTAFLVEAIA
ECCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHHH
RTAGKAMGVIGTAGMMLGREVLEGKSGYTTPEPQTIHRLLAAMLQRGAWGAVMEVSSHAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EQRRTDHCRFDVAVFTNLTHEHLDYHGDMEGYYAAKARLFRGLQPGAAAVVNIDDPYGRR
HHHCCCCCEEEEHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
LAEEIPAGVRTLTYGFSEGADIRAEDVHLTGPGARYRLITPVGEADVEAPHLFGTYNVSN
HHHHCCCCCEEEEECCCCCCCCEECEEEEECCCCCEEEECCCCCCCCCCCCEEEECCHHH
AMAAVGAGLALGFSLQDAVAALGTAKGAPGRFERIDEGQDFTVVVDYAHTPDGFEKLLSD
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEEECCCCHHHHHHHHH
VARIKEPGSRVIMVFGSAGQRDPSKRPDMGRIAGEYCDVLVLTEEDPRTEDAMEIARQIA
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
SGVARDEVEIHLIEDRVEAIREAIRMARTGDIVLITGKGQETELEVQHPTTWRGDVVEAV
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHH
DALRDRAREEARGVRA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSALDPVQVLEAPDVTVTGIAADSRAVRPGDLFVCIPGAHVDAHQFIPDALRRGAVAVV
CCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCEEEEE
TQRPWAVPTGIGAAVVPDARLALSALADRFYGHPSRALRMVGVTGTNGKTTTAFLVEAIA
ECCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHHH
RTAGKAMGVIGTAGMMLGREVLEGKSGYTTPEPQTIHRLLAAMLQRGAWGAVMEVSSHAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EQRRTDHCRFDVAVFTNLTHEHLDYHGDMEGYYAAKARLFRGLQPGAAAVVNIDDPYGRR
HHHCCCCCEEEEHHHHCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHH
LAEEIPAGVRTLTYGFSEGADIRAEDVHLTGPGARYRLITPVGEADVEAPHLFGTYNVSN
HHHHCCCCCEEEEECCCCCCCCEECEEEEECCCCCEEEECCCCCCCCCCCCEEEECCHHH
AMAAVGAGLALGFSLQDAVAALGTAKGAPGRFERIDEGQDFTVVVDYAHTPDGFEKLLSD
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCEEEEEEECCCCHHHHHHHHH
VARIKEPGSRVIMVFGSAGQRDPSKRPDMGRIAGEYCDVLVLTEEDPRTEDAMEIARQIA
HHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHH
SGVARDEVEIHLIEDRVEAIREAIRMARTGDIVLITGKGQETELEVQHPTTWRGDVVEAV
CCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHH
DALRDRAREEARGVRA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA