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The map label for this gene is sbcD [H]

Identifier: 51599080

GI number: 51599080

Start: 877145

End: 878386

Strand: Direct

Name: sbcD [H]

Synonym: BG0854

Alternate gene names: 51599080

Gene position: 877145-878386 (Clockwise)

Preceding gene: 51599076

Following gene: 51599081

Centisome position: 97.0

GC content: 27.38

Gene sequence:

>1242_bases
GTGAGCAATTATAAAATTCTGCATACTTCAGATTGGCATATTGGAAAAAAAATTGAAAATTTTTCAATACTTAAGGAACA
AAAAAAATTTCTCTCTTTCCTTTTAGAATTTATTAAAAAAGAAAAGATAGATCTTTTACTTGTTGCTGGAGATGTTTATG
ATTCCAAGAGGCCCGGATTTGAAGAGCAAAAATTGGTGAATAATTTTTTTTACGAGCTTTCTTTTACTCCTTGTAAATGG
TGTGTGGTTATTTCTGGAAATCATGACAAAAAAGATTATTTAAGTATTAATAAAAAACTTCTCTCACGTTTTAATTTTTT
TTTAATAACAGAATATGATTCTGATGAGCAAATAGTTTTCTTAAAAGATAATGAAAATCTTAAGTTTATTGTTGTTTGCC
TTCCACACATAAATGAAAGGCTTATTTTGGGGCAAAATTTTGACAATATTTTTGGATTAGAAGATCCGTCTTCCAGTAAA
CTATTTCTTGAAAATTTAGAAAATGCTTACAGAGAAAAGATATCAAGTTTATCTAATTTTTTAGAAAACAAGTATAAAAG
AATTCCTAAAATATTAATGGCGCATTCTTTTTTTGGCAGCAGTAAAAAGATTGATACTTTGGGGGGTAGTTATATTATTC
CTTTTAATGTTTTTGGAAATGGTTTTTCTTATGTTGCTCTTGGGCATATTCATAAATTCATGAAACTAAAGGATAATATT
GTTTATTCAGGATCTCCTATGCAATATTCATTTAATGAGACTCATGATAAATATATAAATGTTTTGCATTTTAATCACAA
TAAATTAATCTTGCAAGAAGCATTTCCTGTGCCGATTTTCAATAAATTAATCTTTGTTAAAGGTTCTTTGAATGAAGTTC
TTGACTTTTTTAATAATTTTAAAAAAGAAGAGTCTTTTACTATTTATTTGAAAATCGAACTTAATGAGGCGGTTGATACT
AGTGCTGAGGAGACTATTTATGATCTAGCAAAGCTTAATTTTATAAATTTAGTTTCTATTTCCTATTCTTTACCCTCAAG
TCAGGATTTACAAGACGATTCCAGCTTTATTGGAGAACTCGAAGTGCTTGAAATGGATGAAAAATATTTTTTTGAAAAAA
AATTGAGACGAGATTTTGAGAATGGTGTTATTAAGGATATTAAATTTAAGGAAGAAGAGCTTATTTCTCTTTTTAACGAG
GTTTTAGCTAAAGGATATTTAGGCGAATATGAGGATAAATAA

Upstream 100 bases:

>100_bases
ACTATTATCAATTTTTTATTTTGCATAAATTCCTTATGTTTATATATTGTATTATAATATATAAACATAATTTAAGAATT
AAAAAAAGTGAGAGTATTTT

Downstream 100 bases:

>100_bases
GCTCATATTTAGAAATATTGCTTCTTATAAAGGTGAGCATGAGTTAAATTTCGATACTCTTCTTTTAAGGCAATCAGGTA
TTTTTTTAATTTCTGGTAAT

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSNYKILHTSDWHIGKKIENFSILKEQKKFLSFLLEFIKKEKIDLLLVAGDVYDSKRPGFEEQKLVNNFFYELSFTPCKW
CVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVFLKDNENLKFIVVCLPHINERLILGQNFDNIFGLEDPSSSK
LFLENLENAYREKISSLSNFLENKYKRIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLKDNI
VYSGSPMQYSFNETHDKYINVLHFNHNKLILQEAFPVPIFNKLIFVKGSLNEVLDFFNNFKKEESFTIYLKIELNEAVDT
SAEETIYDLAKLNFINLVSISYSLPSSQDLQDDSSFIGELEVLEMDEKYFFEKKLRRDFENGVIKDIKFKEEELISLFNE
VLAKGYLGEYEDK

Sequences:

>Translated_413_residues
MSNYKILHTSDWHIGKKIENFSILKEQKKFLSFLLEFIKKEKIDLLLVAGDVYDSKRPGFEEQKLVNNFFYELSFTPCKW
CVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVFLKDNENLKFIVVCLPHINERLILGQNFDNIFGLEDPSSSK
LFLENLENAYREKISSLSNFLENKYKRIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLKDNI
VYSGSPMQYSFNETHDKYINVLHFNHNKLILQEAFPVPIFNKLIFVKGSLNEVLDFFNNFKKEESFTIYLKIELNEAVDT
SAEETIYDLAKLNFINLVSISYSLPSSQDLQDDSSFIGELEVLEMDEKYFFEKKLRRDFENGVIKDIKFKEEELISLFNE
VLAKGYLGEYEDK
>Mature_412_residues
SNYKILHTSDWHIGKKIENFSILKEQKKFLSFLLEFIKKEKIDLLLVAGDVYDSKRPGFEEQKLVNNFFYELSFTPCKWC
VVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVFLKDNENLKFIVVCLPHINERLILGQNFDNIFGLEDPSSSKL
FLENLENAYREKISSLSNFLENKYKRIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLKDNIV
YSGSPMQYSFNETHDKYINVLHFNHNKLILQEAFPVPIFNKLIFVKGSLNEVLDFFNNFKKEESFTIYLKIELNEAVDTS
AEETIYDLAKLNFINLVSISYSLPSSQDLQDDSSFIGELEVLEMDEKYFFEKKLRRDFENGVIKDIKFKEEELISLFNEV
LAKGYLGEYEDK

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=350, Percent_Identity=26.2857142857143, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 48336; Mature: 48205

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYKILHTSDWHIGKKIENFSILKEQKKFLSFLLEFIKKEKIDLLLVAGDVYDSKRPGF
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
EEQKLVNNFFYELSFTPCKWCVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVF
HHHHHHHHHHEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEE
LKDNENLKFIVVCLPHINERLILGQNFDNIFGLEDPSSSKLFLENLENAYREKISSLSNF
EECCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LENKYKRIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLKDNI
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCE
VYSGSPMQYSFNETHDKYINVLHFNHNKLILQEAFPVPIFNKLIFVKGSLNEVLDFFNNF
EECCCCCEECCCCCCCCEEEEEEECCCEEEEECCCCCCHHCEEEEEECCHHHHHHHHHHC
KKEESFTIYLKIELNEAVDTSAEETIYDLAKLNFINLVSISYSLPSSQDLQDDSSFIGEL
CCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHCCE
EVLEMDEKYFFEKKLRRDFENGVIKDIKFKEEELISLFNEVLAKGYLGEYEDK
EEEECCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNYKILHTSDWHIGKKIENFSILKEQKKFLSFLLEFIKKEKIDLLLVAGDVYDSKRPGF
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
EEQKLVNNFFYELSFTPCKWCVVISGNHDKKDYLSINKKLLSRFNFFLITEYDSDEQIVF
HHHHHHHHHHEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCEEEE
LKDNENLKFIVVCLPHINERLILGQNFDNIFGLEDPSSSKLFLENLENAYREKISSLSNF
EECCCCEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LENKYKRIPKILMAHSFFGSSKKIDTLGGSYIIPFNVFGNGFSYVALGHIHKFMKLKDNI
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCE
VYSGSPMQYSFNETHDKYINVLHFNHNKLILQEAFPVPIFNKLIFVKGSLNEVLDFFNNF
EECCCCCEECCCCCCCCEEEEEEECCCEEEEECCCCCCHHCEEEEEECCHHHHHHHHHHC
KKEESFTIYLKIELNEAVDTSAEETIYDLAKLNFINLVSISYSLPSSQDLQDDSSFIGEL
CCCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHCCE
EVLEMDEKYFFEKKLRRDFENGVIKDIKFKEEELISLFNEVLAKGYLGEYEDK
EEEECCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]