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The map label for this gene is pnpA [H]

Identifier: 51599057

GI number: 51599057

Start: 850735

End: 852882

Strand: Direct

Name: pnpA [H]

Synonym: BG0831

Alternate gene names: 51599057

Gene position: 850735-852882 (Clockwise)

Preceding gene: 51599056

Following gene: 51599058

Centisome position: 94.08

GC content: 34.36

Gene sequence:

>2148_bases
TTGAAAATAGGTAGAGACGAGTTAGTGTTTGAGACGGGATTTATGGCTAAACAGGCTAATGGATCAGTTCTTGCAACTTA
TGGAGGATCTTCAGTTCTTGCGACTGTTTGTTGTTCAAACAATGTTAGAGAAGATTTAGATTTTGTTCCACTTTCTGTTG
AATATAATGAGAAATATTATGCAGCCGGTAAGATTCCAGGAGGATTTATTAAAAGAGAAGGAAAGCCAAAGGATAAAGAA
ATACTTGTTTCTAGGTTAATAGATAGGCCAATGAGACCCCTTTTTGATAAAAGATTTGGTCGAGAGATTCAAGTAATTCC
TACGACTTTAGCTACAGATCAGCTCAATCCTCCTGATATTGTTGGAATGAATGCTGCTTTTACAGCAGTTTTTTTATCAG
ATATTCCGTTTAATGGTCCAATTGCAGCTGTTAGGATGGTTTATTTAAATGGTAAGTTTATAGTAAATCCTTCTTTTGAA
GAGATTCATGATTCTGATCTTGATATTGTTGTTGCTGGAAGTTTAAATGGAATTACAATGGTAGAAGGTGGTGCTAATGA
GGTTAGTGAGGATATTTTACTGTCAGCAATAGACGGTGCACATGAATATATTAAACAAATTTGCAATGCTCAAAAAGAAT
TTTTAGATATTGTAGGGGAGAAGGAAAAACTTCCTTTAGCTTTTGAAGAAAAAATATTTGAATTTAAGGAGGAGCTTAAG
GATTTTATTTATACTGATCTTAAAGAGGCTTGTTTTGTTAAGGGAAAGCTTAATAGAGATAAGGCTATAACTTTACTGCG
AAATAAATCTTATGAGCATTTTTCTTCTCTTGAGAAATTGACTGATAGTAATGAGTCTCTTTTTTATAAAGCTTTTGATG
ATTTTGAGAGAGAGATTGTTAGAAATTCTATTCTTAACGATAAAATTAGAACAGATGGTCGAACTCCTAATGAGATAAGA
GATATTATTGCAGAAGTTGATATTTTGAGTAGAACGCATGGATCTGCGCTTTTTACAAGAGGAGAGACACAGGCTTTAGC
AGTAACTACTCTTGGCACAAGCATTGATGAGCAAATAATGGATGATATTGATGGCGACAAGCGTCTTAATTTTATGCTTC
ATTACAATTTTCCCCCATTTTCAGTTGGTGAGACCGGTAGGCTCATGACTGGTAGGCGTGAGATTGGTCATGGTCATTTA
GCCCAAAGAGCTTTAGAATCAATGGTTCCTGGAAAAAACGATTTTCCGTATACTATTAGGGTAGTTTCTGAAATTTTGGA
GTCTAACGGATCTTCTTCAATGGCTACTGTTTGTGCTGGAAGCATGTCTTTAATGTCTGCAGGAGTTCCTGTTAAAAGAC
AAGTTGCAGGAATAGCCATGGGGCTTATTAGTGAAGGGGATAAATATGTAGTTTTGAGTGATATTCTTGGAGAAGAGGAT
CATTTAGGTGATATGGACTTTAAAGTGGCTGGTACAAAAAATGGAATCACTGGATTTCAAATGGATATTAAAATTGAAAA
TGTTACTAAGCATTTAATGAGGGATGCTCTTGAGCAGGCAAGAATAGGTAGGATGCACATACTGTCTGTTATGGATACTG
TAATTTCGGATTCAAGAGTTGGTATATCCAAGTATGCTCCCAAAATTGTTCAATTGCAAATTGATATTGACAAGATATCT
CTTGTAATAGGATCTACTGGAAAAACTGTTAAGGCTATAACAGATGAATTTGAAGTTAAGGTTCAAATTGAGCAAAATGG
GAAAATTATTCTTTTCGGTGATGATGATTTTAAGATGCAAAAGGCTAAAGAAAGAATAGAGAGTATTGTAAGGGAACCAA
AGGTAGGTGAAATTTATGAAGGAATCGTTAAAAAGATTAATAGCTTTGGAGCTTTTATTGAACTTACTCCTACAAAGGAA
GGATTTTTAAGTACTCGTTTGAAATCTAGAGACAATAAGTATGGTTCTGGAAGATTTGGGACTGGTAATAGATATCCCAG
ATTTGGTGGTGGAGATAATATAAGAGGTAATGTAGGGTTGGTTCGCCCTCCAAAATTAGAAGAAGGTCAGCAAATTAAAG
TTAAAATAATTGATATAGATAAATTTGGAAAAATTGATCTTGAAGTTGTTAGAGATAAAGATTATTAA

Upstream 100 bases:

>100_bases
GCTATTACCAGAAGAAAGATTTAGAAGCTTATAGAATATTGATATCTAAACTTGGTCTTAGAAAATAAAAAGAGGTTGAA
TTTTGAGGAAAATATTAAAG

Downstream 100 bases:

>100_bases
AATTAATATGAAATTTGTTTTGAGTAATTTATTTAGAGGTTGTCTTATATGCTTTGTCTTATTTTTTTCCTGTCTTACTA
CAAATAAATCTATTCAAGAT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSNNVREDLDFVPLSVEYNEKYYAAGKIPGGFIKREGKPKDKE
ILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFIVNPSFE
EIHDSDLDIVVAGSLNGITMVEGGANEVSEDILLSAIDGAHEYIKQICNAQKEFLDIVGEKEKLPLAFEEKIFEFKEELK
DFIYTDLKEACFVKGKLNRDKAITLLRNKSYEHFSSLEKLTDSNESLFYKAFDDFEREIVRNSILNDKIRTDGRTPNEIR
DIIAEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREIGHGHL
AQRALESMVPGKNDFPYTIRVVSEILESNGSSSMATVCAGSMSLMSAGVPVKRQVAGIAMGLISEGDKYVVLSDILGEED
HLGDMDFKVAGTKNGITGFQMDIKIENVTKHLMRDALEQARIGRMHILSVMDTVISDSRVGISKYAPKIVQLQIDIDKIS
LVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQKAKERIESIVREPKVGEIYEGIVKKINSFGAFIELTPTKE
GFLSTRLKSRDNKYGSGRFGTGNRYPRFGGGDNIRGNVGLVRPPKLEEGQQIKVKIIDIDKFGKIDLEVVRDKDY

Sequences:

>Translated_715_residues
MKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSNNVREDLDFVPLSVEYNEKYYAAGKIPGGFIKREGKPKDKE
ILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFIVNPSFE
EIHDSDLDIVVAGSLNGITMVEGGANEVSEDILLSAIDGAHEYIKQICNAQKEFLDIVGEKEKLPLAFEEKIFEFKEELK
DFIYTDLKEACFVKGKLNRDKAITLLRNKSYEHFSSLEKLTDSNESLFYKAFDDFEREIVRNSILNDKIRTDGRTPNEIR
DIIAEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREIGHGHL
AQRALESMVPGKNDFPYTIRVVSEILESNGSSSMATVCAGSMSLMSAGVPVKRQVAGIAMGLISEGDKYVVLSDILGEED
HLGDMDFKVAGTKNGITGFQMDIKIENVTKHLMRDALEQARIGRMHILSVMDTVISDSRVGISKYAPKIVQLQIDIDKIS
LVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQKAKERIESIVREPKVGEIYEGIVKKINSFGAFIELTPTKE
GFLSTRLKSRDNKYGSGRFGTGNRYPRFGGGDNIRGNVGLVRPPKLEEGQQIKVKIIDIDKFGKIDLEVVRDKDY
>Mature_715_residues
MKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSNNVREDLDFVPLSVEYNEKYYAAGKIPGGFIKREGKPKDKE
ILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDIVGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFIVNPSFE
EIHDSDLDIVVAGSLNGITMVEGGANEVSEDILLSAIDGAHEYIKQICNAQKEFLDIVGEKEKLPLAFEEKIFEFKEELK
DFIYTDLKEACFVKGKLNRDKAITLLRNKSYEHFSSLEKLTDSNESLFYKAFDDFEREIVRNSILNDKIRTDGRTPNEIR
DIIAEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIMDDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREIGHGHL
AQRALESMVPGKNDFPYTIRVVSEILESNGSSSMATVCAGSMSLMSAGVPVKRQVAGIAMGLISEGDKYVVLSDILGEED
HLGDMDFKVAGTKNGITGFQMDIKIENVTKHLMRDALEQARIGRMHILSVMDTVISDSRVGISKYAPKIVQLQIDIDKIS
LVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQKAKERIESIVREPKVGEIYEGIVKKINSFGAFIELTPTKE
GFLSTRLKSRDNKYGSGRFGTGNRYPRFGGGDNIRGNVGLVRPPKLEEGQQIKVKIIDIDKFGKIDLEVVRDKDY

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=682, Percent_Identity=35.1906158357771, Blast_Score=406, Evalue=1e-113,
Organism=Escherichia coli, GI145693187, Length=709, Percent_Identity=42.1720733427362, Blast_Score=556, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI115534063, Length=653, Percent_Identity=34.1500765696784, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI281362905, Length=667, Percent_Identity=35.5322338830585, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24651641, Length=667, Percent_Identity=35.5322338830585, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24651643, Length=667, Percent_Identity=35.5322338830585, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI161079377, Length=611, Percent_Identity=35.8428805237316, Blast_Score=367, Evalue=1e-101,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 79535; Mature: 79535

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSNNVREDLDFVPLSVEYNEKYY
CCCCCCCEEEECCCCEECCCCCEEEEECCCHHHHHHHHCCCCHHCCCEEEEEEEECCEEE
AAGKIPGGFIKREGKPKDKEILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDI
EECCCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCE
VGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFIVNPSFEEIHDSDLDIVVAGSLNGITM
EECCHHHHHHHHHCCCCCCCHHEEEEEEECCEEEECCCHHHHCCCCCCEEEECCCCCEEE
VEGGANEVSEDILLSAIDGAHEYIKQICNAQKEFLDIVGEKEKLPLAFEEKIFEFKEELK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DFIYTDLKEACFVKGKLNRDKAITLLRNKSYEHFSSLEKLTDSNESLFYKAFDDFEREIV
HHHHHHHHHHHEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
RNSILNDKIRTDGRTPNEIRDIIAEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIM
HHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCHHHHHH
DDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREIGHGHLAQRALESMVPGKNDFPYTIR
HHCCCCCEEEEEEEECCCCCCCCCCCCEECCCHHCCCHHHHHHHHHHHCCCCCCCCCHHH
VVSEILESNGSSSMATVCAGSMSLMSAGVPVKRQVAGIAMGLISEGDKYVVLSDILGEED
HHHHHHHCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEHHHCCCCC
HLGDMDFKVAGTKNGITGFQMDIKIENVTKHLMRDALEQARIGRMHILSVMDTVISDSRV
CCCCCCEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
GISKYAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQ
CHHHHCCEEEEEEEEHEEEEEEECCCCCEEHEECCCEEEEEEEECCCEEEEECCCCHHHH
KAKERIESIVREPKVGEIYEGIVKKINSFGAFIELTPTKEGFLSTRLKSRDNKYGSGRFG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCC
TGNRYPRFGGGDNIRGNVGLVRPPKLEEGQQIKVKIIDIDKFGKIDLEVVRDKDY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCC
>Mature Secondary Structure
MKIGRDELVFETGFMAKQANGSVLATYGGSSVLATVCCSNNVREDLDFVPLSVEYNEKYY
CCCCCCCEEEECCCCEECCCCCEEEEECCCHHHHHHHHCCCCHHCCCEEEEEEEECCEEE
AAGKIPGGFIKREGKPKDKEILVSRLIDRPMRPLFDKRFGREIQVIPTTLATDQLNPPDI
EECCCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEEEEECCCCCCCCE
VGMNAAFTAVFLSDIPFNGPIAAVRMVYLNGKFIVNPSFEEIHDSDLDIVVAGSLNGITM
EECCHHHHHHHHHCCCCCCCHHEEEEEEECCEEEECCCHHHHCCCCCCEEEECCCCCEEE
VEGGANEVSEDILLSAIDGAHEYIKQICNAQKEFLDIVGEKEKLPLAFEEKIFEFKEELK
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DFIYTDLKEACFVKGKLNRDKAITLLRNKSYEHFSSLEKLTDSNESLFYKAFDDFEREIV
HHHHHHHHHHHEECCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
RNSILNDKIRTDGRTPNEIRDIIAEVDILSRTHGSALFTRGETQALAVTTLGTSIDEQIM
HHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCHHHHHH
DDIDGDKRLNFMLHYNFPPFSVGETGRLMTGRREIGHGHLAQRALESMVPGKNDFPYTIR
HHCCCCCEEEEEEEECCCCCCCCCCCCEECCCHHCCCHHHHHHHHHHHCCCCCCCCCHHH
VVSEILESNGSSSMATVCAGSMSLMSAGVPVKRQVAGIAMGLISEGDKYVVLSDILGEED
HHHHHHHCCCCCCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEHHHCCCCC
HLGDMDFKVAGTKNGITGFQMDIKIENVTKHLMRDALEQARIGRMHILSVMDTVISDSRV
CCCCCCEEEECCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
GISKYAPKIVQLQIDIDKISLVIGSTGKTVKAITDEFEVKVQIEQNGKIILFGDDDFKMQ
CHHHHCCEEEEEEEEHEEEEEEECCCCCEEHEECCCEEEEEEEECCCEEEEECCCCHHHH
KAKERIESIVREPKVGEIYEGIVKKINSFGAFIELTPTKEGFLSTRLKSRDNKYGSGRFG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCC
TGNRYPRFGGGDNIRGNVGLVRPPKLEEGQQIKVKIIDIDKFGKIDLEVVRDKDY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA