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The map label for this gene is nusA [H]

Identifier: 51599052

GI number: 51599052

Start: 844927

End: 846375

Strand: Direct

Name: nusA [H]

Synonym: BG0826

Alternate gene names: 51599052

Gene position: 844927-846375 (Clockwise)

Preceding gene: 51599051

Following gene: 51599053

Centisome position: 93.44

GC content: 31.61

Gene sequence:

>1449_bases
ATGATAAAGGGCACGGGACATATGATTGTAAGTATTGCAAATGATCGCAGTATGAGTATAGAGTCTATTAGAAAAACAAT
TAAAGAATCAGTTTTGATAGCTTATAAAAAGTACTTTGGAAGCAATGAGAATGCTTTTGTTAAGTTTGATGATAATACGG
GAGATTTAGTTGTTTATGCAAAAAAGAAAATTGTAGAAGAGGTGAAAGATTCTTTACTTGAAATATTAGAAAAAGATATC
TCAAAGGAAAATATTGTAGAAGGTGATTATGCTTATATTGAAATTAATCCCAAGGTTTTTGATAGGCTTTCTATTCAGGT
TGCAAAACAAAGAACCAAAAATGATTTGCAAGGAATTGAGGATAATGAGATTTTATCAGAATTTAAAAGTAAGTTAAATA
AGGTTGTTATTGGATATGTTCAACAAAATAGAAATGGCGATCTTTATGTCAATCTTGGCAATACGGATGGCATAATACCT
AAGAAGTATCAGTCGCCAAGAGAGGTTTATAGCCTTAATGATAAGATTAGAGTTTTGGTTTATAATGTCAAAAAGGGTAA
AAATGGTATTGAAGTTATTCTTTCTAGGACTCATCCAAAGTTTATTGAAGAGCTTTTAGCACTTGAAATTCCTGAAATTG
AAGAAGGTATTATTAAAATTCATAAAATAGTTCGTGATCCGGGGTACAGAATTAAAGTTGCTGTTTATTCTGAAAAAGAA
GAGATTGATCCTGTTGGTCCTTGTATAGGACAAAAAGGAGTTAGAATTCAATCTATAATTAAGGAACTTGAAGGAGAAAA
AATTGATATTATCCCTTACAGTAAAGACATTAAAGAATTCATAAAGGATTCTTTGACTCCTGCTAAAATAGATCATGTCT
ATATTCTTGACGAGGATTTACATAAGGCTTTAGTAGTTGTTAGCGATGATCAACTTTCCCTTGCTATAGGTAAAATGGGG
CAAAATGTCAGACTTGCTAATAGACTTCTTGACTGGGCTATTGATGTTAAAACTAGCAGTCAATTTGCAGAAATGAAAGC
TAATTCGGAGTTTAAGCAAGAAACACTCGAAATGTTTGATAAAGTTATGCAAGATGTTGTTGAAGAGGAACAATTTGAGG
ATATCAGCAAAATAAGTGATCTTAAATTACTTGATTCTTCTGTGATTTCTAATTTATCAAAAGAGGGACTTGATGATATT
AACAATTTTTTACAGGCAGATGAGGAAGTGCTTTTTAATCTTGGAGTAAGCTATGAAAAGCAAGAAGAAATTAACAAAAT
ATTAAAAGAGGGAATGATAATAATTGCTAATGAAAATGATGAGTCTATGGAAAAGGTAGAAGAAGATCAAGAACTTCTTT
GTCCTGAATGTGGCGTTGTTATTAATGAAAATATGACCTCCTGTCCGGGGTGTAAAATAGGGCTTAGCTTTGAGTTTGAG
GAGGAGTAA

Upstream 100 bases:

>100_bases
TCGAAATCAAAAAGTTTTATTTTTAAAACAGAAAGCAAAGAATTAAATGTTTCTTATAGCGATGTTAAGAAGGCCAGATT
AGTTTAAGGAGGAACTTTGG

Downstream 100 bases:

>100_bases
GTTTGTCGGAAAATATTGATGATATTAAAAATGAAGATAGCAAAAAGATTAAGATTATTAAGCTGAGAAAGAAGGTAGTA
AAGATCGTAACGCATAACGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIKGTGHMIVSIANDRSMSIESIRKTIKESVLIAYKKYFGSNENAFVKFDDNTGDLVVYAKKKIVEEVKDSLLEILEKDI
SKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIEDNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIP
KKYQSPREVYSLNDKIRVLVYNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPAKIDHVYILDEDLHKALVVVSDDQLSLAIGKMG
QNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFDKVMQDVVEEEQFEDISKISDLKLLDSSVISNLSKEGLDDI
NNFLQADEEVLFNLGVSYEKQEEINKILKEGMIIIANENDESMEKVEEDQELLCPECGVVINENMTSCPGCKIGLSFEFE
EE

Sequences:

>Translated_482_residues
MIKGTGHMIVSIANDRSMSIESIRKTIKESVLIAYKKYFGSNENAFVKFDDNTGDLVVYAKKKIVEEVKDSLLEILEKDI
SKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIEDNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIP
KKYQSPREVYSLNDKIRVLVYNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPAKIDHVYILDEDLHKALVVVSDDQLSLAIGKMG
QNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFDKVMQDVVEEEQFEDISKISDLKLLDSSVISNLSKEGLDDI
NNFLQADEEVLFNLGVSYEKQEEINKILKEGMIIIANENDESMEKVEEDQELLCPECGVVINENMTSCPGCKIGLSFEFE
EE
>Mature_482_residues
MIKGTGHMIVSIANDRSMSIESIRKTIKESVLIAYKKYFGSNENAFVKFDDNTGDLVVYAKKKIVEEVKDSLLEILEKDI
SKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIEDNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIP
KKYQSPREVYSLNDKIRVLVYNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPAKIDHVYILDEDLHKALVVVSDDQLSLAIGKMG
QNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFDKVMQDVVEEEQFEDISKISDLKLLDSSVISNLSKEGLDDI
NNFLQADEEVLFNLGVSYEKQEEINKILKEGMIIIANENDESMEKVEEDQELLCPECGVVINENMTSCPGCKIGLSFEFE
EE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=416, Percent_Identity=28.3653846153846, Blast_Score=197, Evalue=1e-51,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54802; Mature: 54802

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGTGHMIVSIANDRSMSIESIRKTIKESVLIAYKKYFGSNENAFVKFDDNTGDLVVYA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEH
KKKIVEEVKDSLLEILEKDISKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
DNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIPKKYQSPREVYSLNDKIRVLV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEEE
YNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPAKIDHVYILDEDL
CCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCC
HKALVVVSDDQLSLAIGKMGQNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFD
CCEEEEEECCCEEEHHHHCCCCHHHHHHHHHHHEECCCCHHHHHHHCCCHHHHHHHHHHH
KVMQDVVEEEQFEDISKISDLKLLDSSVISNLSKEGLDDINNFLQADEEVLFNLGVSYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCH
QEEINKILKEGMIIIANENDESMEKVEEDQELLCPECGVVINENMTSCPGCKIGLSFEFE
HHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEEEC
EE
CC
>Mature Secondary Structure
MIKGTGHMIVSIANDRSMSIESIRKTIKESVLIAYKKYFGSNENAFVKFDDNTGDLVVYA
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEH
KKKIVEEVKDSLLEILEKDISKENIVEGDYAYIEINPKVFDRLSIQVAKQRTKNDLQGIE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
DNEILSEFKSKLNKVVIGYVQQNRNGDLYVNLGNTDGIIPKKYQSPREVYSLNDKIRVLV
HHHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEEE
YNVKKGKNGIEVILSRTHPKFIEELLALEIPEIEEGIIKIHKIVRDPGYRIKVAVYSEKE
EEECCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
EIDPVGPCIGQKGVRIQSIIKELEGEKIDIIPYSKDIKEFIKDSLTPAKIDHVYILDEDL
CCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCEEEEEEECCCC
HKALVVVSDDQLSLAIGKMGQNVRLANRLLDWAIDVKTSSQFAEMKANSEFKQETLEMFD
CCEEEEEECCCEEEHHHHCCCCHHHHHHHHHHHEECCCCHHHHHHHCCCHHHHHHHHHHH
KVMQDVVEEEQFEDISKISDLKLLDSSVISNLSKEGLDDINNFLQADEEVLFNLGVSYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCH
QEEINKILKEGMIIIANENDESMEKVEEDQELLCPECGVVINENMTSCPGCKIGLSFEFE
HHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEEEC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]