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The map label for this gene is cheB-2 [H]

Identifier: 51598819

GI number: 51598819

Start: 582415

End: 583572

Strand: Direct

Name: cheB-2 [H]

Synonym: BG0578

Alternate gene names: 51598819

Gene position: 582415-583572 (Clockwise)

Preceding gene: 51598818

Following gene: 51598820

Centisome position: 64.41

GC content: 30.57

Gene sequence:

>1158_bases
ATGAAGATATTAGTAATTGATATTCAAGGTCTTATAAAGCAGGTTTTTATTAGAGCTTTTTCTAAAGATAATGATGTTGA
GATATTAAATGCTGGCTTTAATTCTTTGAATCTTATTAATGTGTTTTTACAAAAATTTCCAGATTTAGTCATTATTGATG
AGAATACGGCGAGATCTAATTTTGGGAATTCTTTAAATAATGTTCTTAATAATATATCTCTTCCTGTGGTATTTATTGCA
CAAAATGAAATGTTTCCAAATTTTGGATGTCTTGAGCAAAGTAAAGAAAAGGTTAAATTAATAATAAATAAACTTAACTT
TAAGCTTACAGTTGATTTATTTCGTAGCAAATATTTAGCTTTAATAAAGCTTGAGTTGAGAAATCTAGGCAAAAATAAAT
TAATATCTTCTTATGAAGTTAAAAGAATTTCTGCACCCGATTTTTCCAGTCATTCTAAAGTAGAGTTGAGAGGAAATAGC
TTAGATGATTCAAGTATAAGGAAAAGTTATAGAGTTTCTGATGTTATTAATTTTGCTCCCAAAAATGATCCGGATGTTAT
TATTAAATATCAAGGCCTTCTCAATAAGCATAAAACTGGTAAAATTATTGTTGTTGGCTCTTCAACTGGTGGTACAGAGG
CTTTAAGAATTTTTTTAAGCTCTTTTAAAAAAGATTCTCCTCCAATTATTATTGTTCAACATATGCCGGGGGGATTTACA
AAGTCTTTTGCAAAAAACTTAAACAATGAGTTTAATATTGATATTAAAGAAGCTGAGGATGGAGACATTCTTCGTCCAGG
TCTTGTAATAATTGCTAATGGAAGTTATCATTTGATTGTAAAATATGGTAGTGGAAATTATTTTGTAAACTTATTAGATG
GGCCCCTTGTTAGTAGGCATAAGCCTTCTGTAAATGTACTTTTTAGGTCTGCTGCAATGTATGCAGGCTCTAATGCTATT
GGTGTTATTCTTACAGGTATGGGAGATGACGGTGCTATTTGTATGCTTGAAATGAAAAAAAATGGCGCTTATACTATTGC
CCAAGATCAAGAAACGTCTGTTGTTTTTGGTATGCCAATGGAAGCTATAAAAATAGGAGCCGTTTACAAAATTCTTCCTT
TGAGTAAAATAGCTGATCATGTTCTGAGGAGATCTTAG

Upstream 100 bases:

>100_bases
GCTTTGGTTGTTGACATAGATGCAATAACTAAGCTTATAAGATAAAAAAACGAAAATACAGATTTTTGGTTTTAAAGGTT
AAAAAGATTAAAATATAATA

Downstream 100 bases:

>100_bases
TAATGGATAATGACAATAATGACTTTTTTGCTAATGATTATTTGGCAACCTTATTTTATAAACTTGAAGTTTTTGATGAG
AGTGCAAGGCATGTTTATTC

Product: protein-glutamate methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKILVIDIQGLIKQVFIRAFSKDNDVEILNAGFNSLNLINVFLQKFPDLVIIDENTARSNFGNSLNNVLNNISLPVVFIA
QNEMFPNFGCLEQSKEKVKLIINKLNFKLTVDLFRSKYLALIKLELRNLGKNKLISSYEVKRISAPDFSSHSKVELRGNS
LDDSSIRKSYRVSDVINFAPKNDPDVIIKYQGLLNKHKTGKIIVVGSSTGGTEALRIFLSSFKKDSPPIIIVQHMPGGFT
KSFAKNLNNEFNIDIKEAEDGDILRPGLVIIANGSYHLIVKYGSGNYFVNLLDGPLVSRHKPSVNVLFRSAAMYAGSNAI
GVILTGMGDDGAICMLEMKKNGAYTIAQDQETSVVFGMPMEAIKIGAVYKILPLSKIADHVLRRS

Sequences:

>Translated_385_residues
MKILVIDIQGLIKQVFIRAFSKDNDVEILNAGFNSLNLINVFLQKFPDLVIIDENTARSNFGNSLNNVLNNISLPVVFIA
QNEMFPNFGCLEQSKEKVKLIINKLNFKLTVDLFRSKYLALIKLELRNLGKNKLISSYEVKRISAPDFSSHSKVELRGNS
LDDSSIRKSYRVSDVINFAPKNDPDVIIKYQGLLNKHKTGKIIVVGSSTGGTEALRIFLSSFKKDSPPIIIVQHMPGGFT
KSFAKNLNNEFNIDIKEAEDGDILRPGLVIIANGSYHLIVKYGSGNYFVNLLDGPLVSRHKPSVNVLFRSAAMYAGSNAI
GVILTGMGDDGAICMLEMKKNGAYTIAQDQETSVVFGMPMEAIKIGAVYKILPLSKIADHVLRRS
>Mature_385_residues
MKILVIDIQGLIKQVFIRAFSKDNDVEILNAGFNSLNLINVFLQKFPDLVIIDENTARSNFGNSLNNVLNNISLPVVFIA
QNEMFPNFGCLEQSKEKVKLIINKLNFKLTVDLFRSKYLALIKLELRNLGKNKLISSYEVKRISAPDFSSHSKVELRGNS
LDDSSIRKSYRVSDVINFAPKNDPDVIIKYQGLLNKHKTGKIIVVGSSTGGTEALRIFLSSFKKDSPPIIIVQHMPGGFT
KSFAKNLNNEFNIDIKEAEDGDILRPGLVIIANGSYHLIVKYGSGNYFVNLLDGPLVSRHKPSVNVLFRSAAMYAGSNAI
GVILTGMGDDGAICMLEMKKNGAYTIAQDQETSVVFGMPMEAIKIGAVYKILPLSKIADHVLRRS

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR [H]

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=390, Percent_Identity=32.0512820512821, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008248
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest [H]

EC number: =3.1.1.61 [H]

Molecular weight: Translated: 42629; Mature: 42629

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIDIQGLIKQVFIRAFSKDNDVEILNAGFNSLNLINVFLQKFPDLVIIDENTARSN
CEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCC
FGNSLNNVLNNISLPVVFIAQNEMFPNFGCLEQSKEKVKLIINKLNFKLTVDLFRSKYLA
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCEEEEEEHHHCCEEE
LIKLELRNLGKNKLISSYEVKRISAPDFSSHSKVELRGNSLDDSSIRKSYRVSDVINFAP
EEEEEHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
KNDPDVIIKYQGLLNKHKTGKIIVVGSSTGGTEALRIFLSSFKKDSPPIIIVQHMPGGFT
CCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHH
KSFAKNLNNEFNIDIKEAEDGDILRPGLVIIANGSYHLIVKYGSGNYFVNLLDGPLVSRH
HHHHHHCCCEEEEEEEECCCCCEECCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCC
KPSVNVLFRSAAMYAGSNAIGVILTGMGDDGAICMLEMKKNGAYTIAQDQETSVVFGMPM
CCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCEEEECCCCCEEEECCCH
EAIKIGAVYKILPLSKIADHVLRRS
HHHEECCEEEECCHHHHHHHHHHCC
>Mature Secondary Structure
MKILVIDIQGLIKQVFIRAFSKDNDVEILNAGFNSLNLINVFLQKFPDLVIIDENTARSN
CEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCCCCCC
FGNSLNNVLNNISLPVVFIAQNEMFPNFGCLEQSKEKVKLIINKLNFKLTVDLFRSKYLA
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHEEECCCEEEEEEHHHCCEEE
LIKLELRNLGKNKLISSYEVKRISAPDFSSHSKVELRGNSLDDSSIRKSYRVSDVINFAP
EEEEEHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
KNDPDVIIKYQGLLNKHKTGKIIVVGSSTGGTEALRIFLSSFKKDSPPIIIVQHMPGGFT
CCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHH
KSFAKNLNNEFNIDIKEAEDGDILRPGLVIIANGSYHLIVKYGSGNYFVNLLDGPLVSRH
HHHHHHCCCEEEEEEEECCCCCEECCCEEEEECCCEEEEEEECCCCEEEEEECCCCCCCC
KPSVNVLFRSAAMYAGSNAIGVILTGMGDDGAICMLEMKKNGAYTIAQDQETSVVFGMPM
CCCCEEEEEEHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCCEEEECCCCCEEEECCCH
EAIKIGAVYKILPLSKIADHVLRRS
HHHEECCEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]