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The map label for this gene is rpoA [H]

Identifier: 51598757

GI number: 51598757

Start: 510137

End: 511171

Strand: Direct

Name: rpoA [H]

Synonym: BG0514

Alternate gene names: 51598757

Gene position: 510137-511171 (Clockwise)

Preceding gene: 51598756

Following gene: 51598758

Centisome position: 56.42

GC content: 30.43

Gene sequence:

>1035_bases
ATGCCTGTAGAAAAATTTTTGAAAGATTTTACTATACCTGAAAAAATTGAATTTTTGAAAAGCCAAGATGATGGATCTTA
TGGTAAATTTACGATATATCCTTTTGAAAGAGGTTTTGGAGTTACTATAGGTAATACTTTAAGACGCGTGTTGCTTTCTT
CTATTGAAGGGTATGCGATTACTGCTATGAGAATTCAGTCTAACAATAAAGACTCTTCGTCAAAGGTTGTTTCAAGTGAA
TTTGATTTGATTCCTGGAGTTTCTGAAGATACTCTTGAGGTCATTGCTAATATTAAAAATATCCATTTGAAACTTGGAGA
AGGAGAGCAAAGAAAGATAATAAGCTTTAGTGTTAGCGGTAAAGATACTAAGGTTTTGAAAGCTTCTCATTTTGAAAGAG
ATGGCGTTGAGGTTTTTAATAGAGATTTAGTTATAGCTACTTTATCACAGGATGTGAATTTAGATCTTGAATTTCAAATT
AATTATGGTAGAGGCTATGTGTCTTCTGAGCAAAATTCCAAGTATTTAGAAGAAGTTAATGTTATTGCTTTAGATTCTAT
ATTTTCGCCTATAGAGAAAGTTTCATACTCTGTAGAAGATACTAGGGTTGGGCAAAGGTCAGATTATGACAAGCTTGTAA
TGGAAATTTGGACCACAGGCGTGATTTCTGCCAAAGATGCAATAAAAAAAGCCGCATCAATAATAAGAGAGTTTTTATTT
CCTCTTATAGATTTTGAAGATAATATTAATACATCTTTTGAGAAATCAAAATCAGAAAGTTCTAACTTACTTGATATGAG
TATTGAAAAATTGGATTTGTCAGTCAGATCTTTAAATTGTTTAGCTAAAGAAAATGTTAGAACTTTAGGGGAACTTATTA
GTAAAAATGCAGAAGAGCTTTCTAAGGCAAGAAATTTTGGGAAAAAAAGTTTAGAAGAGATAATTGAAAAACTTAGTTCT
TATCAATTGTTTTTAGGAATGTCTAAAGAAGATGCTTTATTTGTATTGAGTAAGAATGTTAAAATATCGGAATAA

Upstream 100 bases:

>100_bases
AATTGGTATGACTGTAAAGTCAATTTCAGATATTACCCCTATTCCTCATAATGGATGCAGACCGAAAAAAACCAGACGAG
TTTAGCTAGAAGGAGTTATG

Downstream 100 bases:

>100_bases
AAGGAGAGTTTAGGTTATTTCATGAAAACAAAATTAGGCTTTAATAGGTTAAGTAGGAAATCTAGTCATAGGAGAGCACT
TTTAAAAAATATGGTAATTT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MPVEKFLKDFTIPEKIEFLKSQDDGSYGKFTIYPFERGFGVTIGNTLRRVLLSSIEGYAITAMRIQSNNKDSSSKVVSSE
FDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQI
NYGRGYVSSEQNSKYLEEVNVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIIREFLF
PLIDFEDNINTSFEKSKSESSNLLDMSIEKLDLSVRSLNCLAKENVRTLGELISKNAEELSKARNFGKKSLEEIIEKLSS
YQLFLGMSKEDALFVLSKNVKISE

Sequences:

>Translated_344_residues
MPVEKFLKDFTIPEKIEFLKSQDDGSYGKFTIYPFERGFGVTIGNTLRRVLLSSIEGYAITAMRIQSNNKDSSSKVVSSE
FDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQI
NYGRGYVSSEQNSKYLEEVNVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIIREFLF
PLIDFEDNINTSFEKSKSESSNLLDMSIEKLDLSVRSLNCLAKENVRTLGELISKNAEELSKARNFGKKSLEEIIEKLSS
YQLFLGMSKEDALFVLSKNVKISE
>Mature_343_residues
PVEKFLKDFTIPEKIEFLKSQDDGSYGKFTIYPFERGFGVTIGNTLRRVLLSSIEGYAITAMRIQSNNKDSSSKVVSSEF
DLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSGKDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQIN
YGRGYVSSEQNSKYLEEVNVIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIIREFLFP
LIDFEDNINTSFEKSKSESSNLLDMSIEKLDLSVRSLNCLAKENVRTLGELISKNAEELSKARNFGKKSLEEIIEKLSSY
QLFLGMSKEDALFVLSKNVKISE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=326, Percent_Identity=35.5828220858896, Blast_Score=191, Evalue=4e-50,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 38716; Mature: 38585

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVEKFLKDFTIPEKIEFLKSQDDGSYGKFTIYPFERGFGVTIGNTLRRVLLSSIEGYAI
CCHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCEEE
TAMRIQSNNKDSSSKVVSSEFDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSG
EEEEEECCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEEEECC
KDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQINYGRGYVSSEQNSKYLEEVN
CCCEEEECCCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHC
VIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIIREFLF
EEEHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PLIDFEDNINTSFEKSKSESSNLLDMSIEKLDLSVRSLNCLAKENVRTLGELISKNAEEL
HHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SKARNFGKKSLEEIIEKLSSYQLFLGMSKEDALFVLSKNVKISE
HHHHHCCHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCEECC
>Mature Secondary Structure 
PVEKFLKDFTIPEKIEFLKSQDDGSYGKFTIYPFERGFGVTIGNTLRRVLLSSIEGYAI
CHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHHCCCEEE
TAMRIQSNNKDSSSKVVSSEFDLIPGVSEDTLEVIANIKNIHLKLGEGEQRKIISFSVSG
EEEEEECCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEEEECC
KDTKVLKASHFERDGVEVFNRDLVIATLSQDVNLDLEFQINYGRGYVSSEQNSKYLEEVN
CCCEEEECCCCCCCCHHHHCCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCHHHHHHHC
VIALDSIFSPIEKVSYSVEDTRVGQRSDYDKLVMEIWTTGVISAKDAIKKAASIIREFLF
EEEHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
PLIDFEDNINTSFEKSKSESSNLLDMSIEKLDLSVRSLNCLAKENVRTLGELISKNAEEL
HHHCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SKARNFGKKSLEEIIEKLSSYQLFLGMSKEDALFVLSKNVKISE
HHHHHCCHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]