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The map label for this gene is greA [H]

Identifier: 50842047

GI number: 50842047

Start: 621380

End: 621886

Strand: Reverse

Name: greA [H]

Synonym: PPA0561

Alternate gene names: 50842047

Gene position: 621886-621380 (Counterclockwise)

Preceding gene: 50842048

Following gene: 50842044

Centisome position: 24.29

GC content: 60.95

Gene sequence:

>507_bases
ATGCCTGAGTCTGAAGACACTGTGTGGCTGACCCAAGAGGCCTTCGATAAGCTCACCCAGGAGCTGGAGTACCTCAAAGG
CGAAGGCCGCACCGCCATTGCCAACAAGATTGCCGACGCCCGTTCGGAAGGCGACCTTTCTGAGAACGGCGGCTACCATG
CCGCCCGTGAGGAGCAGGGGCAGGCCGAGGCCCGCATCCGTCAACTCGAGCAGATGCTGCGTAACGCTGAGGTGGGCGAG
GCCCCGTCCTCTCCCGACGAGGTCGTGCCAGGTTCCAAGGTGACGGTCTGCTACTTTGACGACGAGTCCGACACCGACAC
TTTCCTGCTGGGATCGCGTCAGGTGATGGGCCTTGACGATTCCGTCAACGTCTCCGTCTATTCGCCGCAATCTCCCCTTG
GCGGCGCCATTTTGGGCCATTCGAAGGGCGAGTCGACCGAATACCAGGCTCCCAATGGCAAGACGATCAAGGTGACGATC
CTTAACGTCGAGCCGTTCGAGGAGTGA

Upstream 100 bases:

>100_bases
TGAAACAATCCGCGCCAGCGTTAAGACCTTCTCGCGGGCTGTCACCGCCGATCTGGAGAAGTGTGGACCGATCAGGTGAC
ACTCGCGGTAGACTGAATAG

Downstream 100 bases:

>100_bases
CACTCACCAATCGTCACACGCATTGTCGTACAACATCGCTAATCGCTATGAGGGCAGGGCGCCATGCACCCTGCCCTCAT
CGTGTTCTGCCTGATATCGC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MPESEDTVWLTQEAFDKLTQELEYLKGEGRTAIANKIADARSEGDLSENGGYHAAREEQGQAEARIRQLEQMLRNAEVGE
APSSPDEVVPGSKVTVCYFDDESDTDTFLLGSRQVMGLDDSVNVSVYSPQSPLGGAILGHSKGESTEYQAPNGKTIKVTI
LNVEPFEE

Sequences:

>Translated_168_residues
MPESEDTVWLTQEAFDKLTQELEYLKGEGRTAIANKIADARSEGDLSENGGYHAAREEQGQAEARIRQLEQMLRNAEVGE
APSSPDEVVPGSKVTVCYFDDESDTDTFLLGSRQVMGLDDSVNVSVYSPQSPLGGAILGHSKGESTEYQAPNGKTIKVTI
LNVEPFEE
>Mature_167_residues
PESEDTVWLTQEAFDKLTQELEYLKGEGRTAIANKIADARSEGDLSENGGYHAAREEQGQAEARIRQLEQMLRNAEVGEA
PSSPDEVVPGSKVTVCYFDDESDTDTFLLGSRQVMGLDDSVNVSVYSPQSPLGGAILGHSKGESTEYQAPNGKTIKVTIL
NVEPFEE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=161, Percent_Identity=32.2981366459627, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI87082261, Length=146, Percent_Identity=28.7671232876712, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18312; Mature: 18181

Theoretical pI: Translated: 4.05; Mature: 4.05

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESEDTVWLTQEAFDKLTQELEYLKGEGRTAIANKIADARSEGDLSENGGYHAAREEQG
CCCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QAEARIRQLEQMLRNAEVGEAPSSPDEVVPGSKVTVCYFDDESDTDTFLLGSRQVMGLDD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCEEECCCC
SVNVSVYSPQSPLGGAILGHSKGESTEYQAPNGKTIKVTILNVEPFEE
CCEEEEECCCCCCCCEEEECCCCCCCCEECCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
PESEDTVWLTQEAFDKLTQELEYLKGEGRTAIANKIADARSEGDLSENGGYHAAREEQG
CCCCCCEEECHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
QAEARIRQLEQMLRNAEVGEAPSSPDEVVPGSKVTVCYFDDESDTDTFLLGSRQVMGLDD
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCEEECCCC
SVNVSVYSPQSPLGGAILGHSKGESTEYQAPNGKTIKVTILNVEPFEE
CCEEEEECCCCCCCCEEEECCCCCCCCEECCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12692562; 11572948 [H]