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The map label for this gene is nagB

Identifier: 50841959

GI number: 50841959

Start: 527278

End: 528063

Strand: Direct

Name: nagB

Synonym: PPA0476

Alternate gene names: 50841959

Gene position: 527278-528063 (Clockwise)

Preceding gene: 50841954

Following gene: 50841960

Centisome position: 20.59

GC content: 61.32

Gene sequence:

>786_bases
ATGGAGGTCATCATCTGCTCGGATGAGCAGGCCGTCGGGCGGATCGCCGCCGACCGGATTGCAAAGGTTTTGGGCCGGGC
CAAGGCTCCGGTGTTGGGAGTCGCGACTGGATCGACCCCGCTGACGACCTACTCTTCCCTGGCTGCTCTGGCAGCCGAGG
GTAAGGCTGACTTTACTGATCTGCGCGCCTTTGCCCTTGACGAATACATTGGGCTTCCTGCAGACGATGAGCGCTCCTAC
GCAGCTACCATCCGCCACACCGTCACCGAACAGCTGGGACTCGATCCGGCCAACGTCCACACCCCCGACGGCATGGCCGA
TGACCTCGATGCTGCCTGCGCCGCATACGAGACAGCCATCCAGGAGGCTGATGGCGTCGATGTTCAGATCCTTGGCATTG
GGGCAAATGGACATATTGGTTTCAATGAGCCCACCAGTTCGCTGTCTTCGCGCACTCGCGTCAAGACCCTTGCGGAGCGC
ACAAAGGCTGACAATGCCCGCTTCTTTGAGGAGGGGGAGAAGGTCCCGAGTCACTGCGTCACCCAGGGGCTCGGTACCAT
CATGGACGCTCGCAATGTTGTGCTGTTGGCCATCGGAGCCAACAAGGCTGACGCGCTGGCAGGGGCTGTCGAGGGGCCTG
TTTCGGCGATGTGCCCGGCGTCGGTGCTGCAGTTCCACCCCCATGTTTTGGTGATTGCGGACGATGCTGCAGCCTCGAAG
CTCACCATGGCTGAGTACTTCCGATGGACAGATTCCCAGCGAGGTGACCTCTCCGGAGCGATGTGA

Upstream 100 bases:

>100_bases
GTGTGATCGCTCAAGAACGCTTACATGGCCCTAGTCTCACTTCAGGCACGTCCCAGCTAGGACGTGGCGCACGCTGTGAT
CGCGTCAAGGAAGGTTCGAC

Downstream 100 bases:

>100_bases
GATTTCGGTGATACTCCTGGCCTGGTAGGTGTCTTCGCGCCGGGCTATCGAAGTATCGGATAATGGTGAGTGGTGGACCG
TCGTCAAGGCTTGTAGCCTG

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MEVIICSDEQAVGRIAADRIAKVLGRAKAPVLGVATGSTPLTTYSSLAALAAEGKADFTDLRAFALDEYIGLPADDERSY
AATIRHTVTEQLGLDPANVHTPDGMADDLDAACAAYETAIQEADGVDVQILGIGANGHIGFNEPTSSLSSRTRVKTLAER
TKADNARFFEEGEKVPSHCVTQGLGTIMDARNVVLLAIGANKADALAGAVEGPVSAMCPASVLQFHPHVLVIADDAAASK
LTMAEYFRWTDSQRGDLSGAM

Sequences:

>Translated_261_residues
MEVIICSDEQAVGRIAADRIAKVLGRAKAPVLGVATGSTPLTTYSSLAALAAEGKADFTDLRAFALDEYIGLPADDERSY
AATIRHTVTEQLGLDPANVHTPDGMADDLDAACAAYETAIQEADGVDVQILGIGANGHIGFNEPTSSLSSRTRVKTLAER
TKADNARFFEEGEKVPSHCVTQGLGTIMDARNVVLLAIGANKADALAGAVEGPVSAMCPASVLQFHPHVLVIADDAAASK
LTMAEYFRWTDSQRGDLSGAM
>Mature_261_residues
MEVIICSDEQAVGRIAADRIAKVLGRAKAPVLGVATGSTPLTTYSSLAALAAEGKADFTDLRAFALDEYIGLPADDERSY
AATIRHTVTEQLGLDPANVHTPDGMADDLDAACAAYETAIQEADGVDVQILGIGANGHIGFNEPTSSLSSRTRVKTLAER
TKADNARFFEEGEKVPSHCVTQGLGTIMDARNVVLLAIGANKADALAGAVEGPVSAMCPASVLQFHPHVLVIADDAAASK
LTMAEYFRWTDSQRGDLSGAM

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI19923881, Length=256, Percent_Identity=39.84375, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI13027378, Length=256, Percent_Identity=41.40625, Blast_Score=186, Evalue=1e-47,
Organism=Escherichia coli, GI1786893, Length=265, Percent_Identity=35.0943396226415, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI48994958, Length=235, Percent_Identity=29.7872340425532, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789530, Length=224, Percent_Identity=27.6785714285714, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17554876, Length=255, Percent_Identity=40.3921568627451, Blast_Score=186, Evalue=8e-48,
Organism=Drosophila melanogaster, GI24581960, Length=256, Percent_Identity=41.796875, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI19920764, Length=256, Percent_Identity=41.796875, Blast_Score=194, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_PROAC (Q6AAI8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055186.1
- HSSP:   P46926
- ProteinModelPortal:   Q6AAI8
- SMR:   Q6AAI8
- GeneID:   2932321
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0476
- NMPDR:   fig|267747.1.peg.463
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- BioCyc:   PACN267747:PPA0476-MONOMER
- BRENDA:   3.5.99.6
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 27331; Mature: 27331

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 67-67 ACT_SITE 136-136 ACT_SITE 138-138 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVIICSDEQAVGRIAADRIAKVLGRAKAPVLGVATGSTPLTTYSSLAALAAEGKADFTD
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHH
LRAFALDEYIGLPADDERSYAATIRHTVTEQLGLDPANVHTPDGMADDLDAACAAYETAI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QEADGVDVQILGIGANGHIGFNEPTSSLSSRTRVKTLAERTKADNARFFEEGEKVPSHCV
HHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHH
TQGLGTIMDARNVVLLAIGANKADALAGAVEGPVSAMCPASVLQFHPHVLVIADDAAASK
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHHHHCHHHHHHHCCCEEEEECCCCHHH
LTMAEYFRWTDSQRGDLSGAM
HHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEVIICSDEQAVGRIAADRIAKVLGRAKAPVLGVATGSTPLTTYSSLAALAAEGKADFTD
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHH
LRAFALDEYIGLPADDERSYAATIRHTVTEQLGLDPANVHTPDGMADDLDAACAAYETAI
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
QEADGVDVQILGIGANGHIGFNEPTSSLSSRTRVKTLAERTKADNARFFEEGEKVPSHCV
HHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHH
TQGLGTIMDARNVVLLAIGANKADALAGAVEGPVSAMCPASVLQFHPHVLVIADDAAASK
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCHHHHCHHHHHHHCCCEEEEECCCCHHH
LTMAEYFRWTDSQRGDLSGAM
HHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA