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The map label for this gene is cobS [H]

Identifier: 50841925

GI number: 50841925

Start: 487055

End: 487942

Strand: Direct

Name: cobS [H]

Synonym: PPA0442

Alternate gene names: 50841925

Gene position: 487055-487942 (Clockwise)

Preceding gene: 50841924

Following gene: 50841926

Centisome position: 19.02

GC content: 63.06

Gene sequence:

>888_bases
ATGCGACGAGGTGTGGATGTGTGTGGCCGGAATCCCCAAGAGGTGGGCATGACGGACGAGCAGCATCCGGATCTTGGGAG
ACTCGGGCCACTGCGTGGAGTTGCTGAGGCTTTGTCTCTCTTTACGATCTTGCCCGGGCCATATCTGAACGACATTGACC
GTCCACTAGCCCGTCGTGCCATTACCGCCTTCCCGTGGCTGGGTCTGTGTCTGGGCGTGGTGGCGGGAGGGATCGTCGCC
CTGGTCCTGGGACTGGGGGCCGGGCCGTGGCTTGCCGGAGTGCTAGCGCTGGGCTGGCTTGCCGGCGCGACGGGCGGCTT
CCATCTCGACGGGGTCGCTGACACCGCAGATGGTTTGGGATCCCGGGCGGCGCCGGAGAAGGCTCTCATGATCATGAAGA
AGTCCGATATCGGGCCGATGGGTGTCATGAGTATCGTGCTGGTGCTACTTGTTGACGCGGCCGCTGTGGTGAGTCTGGCT
GGGACAGCTGGCTCGGGGGCATGGTGGCGCGTGGCGATTCTGGTGGGACTTGGCCCTGCGCTGGCTCGTGCGGCGATCCT
TCTCGCGACGATGACCGAAGTGCCGTGTGCCCGTCCGGGCGGGTTCGGGTCTTTAGTTGCGGGAGTGACGACGCCGAGGT
CTGCGGCGATCAACCTCACGGTGTTGGGATGCGTGTGTGCCTTAGCCGGATTGGTGGCTGGAGGCTGGATGTGGTGCTTG
GTAACGGTCAGCTGTGCCGCGCTGACCTTGCTTATCGCGCGAGGATGGATTCATCACCTAGTGCGACGCCTATCCGGGAT
GACGGGTGACACATTTGGAAGCATTAATGAGGTGACGCAGATGACGTTTTGGGTCCTGACGGCGTTGACCGTGGCGGTGG
TGTCATGA

Upstream 100 bases:

>100_bases
TTGTCTCTAACGAGGTGGGGATGGGGGTCGTGCCCGACACCCCGGCTGGACGGCTCTTCCGCGACGAGTTGGGGCGACTC
AACGCCGCCGTCGGGGAAGC

Downstream 100 bases:

>100_bases
TCGATGATCTTGGTGTGAAGGCTTCGGTAGCGAGACCTGTCCAGCGGGTGGTGAGCTTAGTGCCGTCGATTACCGAAGCT
ATCGCGATGACTTGTCCACA

Product: putative cobalamin-5-phosphate synthase, CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRRGVDVCGRNPQEVGMTDEQHPDLGRLGPLRGVAEALSLFTILPGPYLNDIDRPLARRAITAFPWLGLCLGVVAGGIVA
LVLGLGAGPWLAGVLALGWLAGATGGFHLDGVADTADGLGSRAAPEKALMIMKKSDIGPMGVMSIVLVLLVDAAAVVSLA
GTAGSGAWWRVAILVGLGPALARAAILLATMTEVPCARPGGFGSLVAGVTTPRSAAINLTVLGCVCALAGLVAGGWMWCL
VTVSCAALTLLIARGWIHHLVRRLSGMTGDTFGSINEVTQMTFWVLTALTVAVVS

Sequences:

>Translated_295_residues
MRRGVDVCGRNPQEVGMTDEQHPDLGRLGPLRGVAEALSLFTILPGPYLNDIDRPLARRAITAFPWLGLCLGVVAGGIVA
LVLGLGAGPWLAGVLALGWLAGATGGFHLDGVADTADGLGSRAAPEKALMIMKKSDIGPMGVMSIVLVLLVDAAAVVSLA
GTAGSGAWWRVAILVGLGPALARAAILLATMTEVPCARPGGFGSLVAGVTTPRSAAINLTVLGCVCALAGLVAGGWMWCL
VTVSCAALTLLIARGWIHHLVRRLSGMTGDTFGSINEVTQMTFWVLTALTVAVVS
>Mature_295_residues
MRRGVDVCGRNPQEVGMTDEQHPDLGRLGPLRGVAEALSLFTILPGPYLNDIDRPLARRAITAFPWLGLCLGVVAGGIVA
LVLGLGAGPWLAGVLALGWLAGATGGFHLDGVADTADGLGSRAAPEKALMIMKKSDIGPMGVMSIVLVLLVDAAAVVSLA
GTAGSGAWWRVAILVGLGPALARAAILLATMTEVPCARPGGFGSLVAGVTTPRSAAINLTVLGCVCALAGLVAGGWMWCL
VTVSCAALTLLIARGWIHHLVRRLSGMTGDTFGSINEVTQMTFWVLTALTVAVVS

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=252, Percent_Identity=32.1428571428571, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 30176; Mature: 30176

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRGVDVCGRNPQEVGMTDEQHPDLGRLGPLRGVAEALSLFTILPGPYLNDIDRPLARRA
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITAFPWLGLCLGVVAGGIVALVLGLGAGPWLAGVLALGWLAGATGGFHLDGVADTADGLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCC
SRAAPEKALMIMKKSDIGPMGVMSIVLVLLVDAAAVVSLAGTAGSGAWWRVAILVGLGPA
CCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
LARAAILLATMTEVPCARPGGFGSLVAGVTTPRSAAINLTVLGCVCALAGLVAGGWMWCL
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVSCAALTLLIARGWIHHLVRRLSGMTGDTFGSINEVTQMTFWVLTALTVAVVS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRGVDVCGRNPQEVGMTDEQHPDLGRLGPLRGVAEALSLFTILPGPYLNDIDRPLARRA
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ITAFPWLGLCLGVVAGGIVALVLGLGAGPWLAGVLALGWLAGATGGFHLDGVADTADGLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCC
SRAAPEKALMIMKKSDIGPMGVMSIVLVLLVDAAAVVSLAGTAGSGAWWRVAILVGLGPA
CCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHH
LARAAILLATMTEVPCARPGGFGSLVAGVTTPRSAAINLTVLGCVCALAGLVAGGWMWCL
HHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVSCAALTLLIARGWIHHLVRRLSGMTGDTFGSINEVTQMTFWVLTALTVAVVS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA