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The map label for this gene is opuCB [H]

Identifier: 50841887

GI number: 50841887

Start: 451252

End: 451947

Strand: Reverse

Name: opuCB [H]

Synonym: PPA0405

Alternate gene names: 50841887

Gene position: 451947-451252 (Counterclockwise)

Preceding gene: 50841888

Following gene: 50841886

Centisome position: 17.65

GC content: 60.63

Gene sequence:

>696_bases
ATGAACCACATCGCAGCAGCTCTCACTTGGTTGACGACTTCCTCCCACTGGGCCGGTTCCGGATCTATCGGGGTCCGCAT
CGGCGAGCACCTTTGGTACACCTTGCTGGCACTCACCATCGCCGCCATCACCGCCATTCCTGCTGGGCTAGCTATCGGTC
ATACCGGTCGTGGAAAGACTATCGCAGTGGCTTGTTCTGGAGCTTTGCGTGCTCTCCCCTCTCTCGGCTTGCTCACTCTG
CTAGCGATCTCGACAACGATCGGAATCGAGAATCCCGTCGTACCTTCCACCATCGTGCTAGCTATCCTGGCGATTCCACC
CCTGCTGGCCGGTAGCTATTCCGGCGTAGACGCCATTGACGACGAGGTAGCTGACGGCGCTCGAGCCTGCGGCATGACAG
AGATGCAGGTACTGGGCCGCGTCGAGTTGCCGCTAGCCACTCCCCTCCTCTTGGGCGGGTTGAGGTCGGCGGCCTTGCAG
GTGATCGCGACGACGACAATATGTTCATATTTGGGCATGGGGGGTCTTGGCCGTTTCATCCTTGACGGGCTGGCGGTCTC
CGACTACCCACAGATGCTCGCCGGATCCATCGTCATCATCGTGCTGGCTCTGGTTGTTGACGGGCTGCTCTGCCTCGTTA
CCCGCTTACTCACTGGCCCGGGTGTCAGACACGCCTTGGCCCTCACCCCGCAGTGA

Upstream 100 bases:

>100_bases
GGGTAGGAATTATCGGGACCCTGGTACTGGCGATCATCTTCGATCTCGTCATCGTCTGCGTCGGTCGCCTCCTGACTCCG
TGGCGGAGGGCAGCGACGAT

Downstream 100 bases:

>100_bases
TCCCATCGAATGGAGCACCCGATGATCACAACGCCCATGACCCGCCGAACCCTGCTCGGAACAGCGTTGCTGACCGGTCT
GGCAGCATGTTCCTCAAACG

Product: ABC transporter-associated permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNHIAAALTWLTTSSHWAGSGSIGVRIGEHLWYTLLALTIAAITAIPAGLAIGHTGRGKTIAVACSGALRALPSLGLLTL
LAISTTIGIENPVVPSTIVLAILAIPPLLAGSYSGVDAIDDEVADGARACGMTEMQVLGRVELPLATPLLLGGLRSAALQ
VIATTTICSYLGMGGLGRFILDGLAVSDYPQMLAGSIVIIVLALVVDGLLCLVTRLLTGPGVRHALALTPQ

Sequences:

>Translated_231_residues
MNHIAAALTWLTTSSHWAGSGSIGVRIGEHLWYTLLALTIAAITAIPAGLAIGHTGRGKTIAVACSGALRALPSLGLLTL
LAISTTIGIENPVVPSTIVLAILAIPPLLAGSYSGVDAIDDEVADGARACGMTEMQVLGRVELPLATPLLLGGLRSAALQ
VIATTTICSYLGMGGLGRFILDGLAVSDYPQMLAGSIVIIVLALVVDGLLCLVTRLLTGPGVRHALALTPQ
>Mature_231_residues
MNHIAAALTWLTTSSHWAGSGSIGVRIGEHLWYTLLALTIAAITAIPAGLAIGHTGRGKTIAVACSGALRALPSLGLLTL
LAISTTIGIENPVVPSTIVLAILAIPPLLAGSYSGVDAIDDEVADGARACGMTEMQVLGRVELPLATPLLLGGLRSAALQ
VIATTTICSYLGMGGLGRFILDGLAVSDYPQMLAGSIVIIVLALVVDGLLCLVTRLLTGPGVRHALALTPQ

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for glycine betaine, carnitine and choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1174

COG function: function code E; ABC-type proline/glycine betaine transport systems, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=173, Percent_Identity=27.1676300578035, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788451, Length=158, Percent_Identity=36.0759493670886, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788449, Length=193, Percent_Identity=27.979274611399, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 23498; Mature: 23498

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS01076 ACETATE_KINASE_2 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHIAAALTWLTTSSHWAGSGSIGVRIGEHLWYTLLALTIAAITAIPAGLAIGHTGRGKT
CCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCE
IAVACSGALRALPSLGLLTLLAISTTIGIENPVVPSTIVLAILAIPPLLAGSYSGVDAID
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
DEVADGARACGMTEMQVLGRVELPLATPLLLGGLRSAALQVIATTTICSYLGMGGLGRFI
HHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LDGLAVSDYPQMLAGSIVIIVLALVVDGLLCLVTRLLTGPGVRHALALTPQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCC
>Mature Secondary Structure
MNHIAAALTWLTTSSHWAGSGSIGVRIGEHLWYTLLALTIAAITAIPAGLAIGHTGRGKT
CCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCE
IAVACSGALRALPSLGLLTLLAISTTIGIENPVVPSTIVLAILAIPPLLAGSYSGVDAID
EEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
DEVADGARACGMTEMQVLGRVELPLATPLLLGGLRSAALQVIATTTICSYLGMGGLGRFI
HHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LDGLAVSDYPQMLAGSIVIIVLALVVDGLLCLVTRLLTGPGVRHALALTPQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10216873; 9384377 [H]