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The map label for this gene is eda [H]

Identifier: 50841856

GI number: 50841856

Start: 419351

End: 419998

Strand: Reverse

Name: eda [H]

Synonym: PPA0371

Alternate gene names: 50841856

Gene position: 419998-419351 (Counterclockwise)

Preceding gene: 50841860

Following gene: 50841855

Centisome position: 16.4

GC content: 67.13

Gene sequence:

>648_bases
GTGAGCAACGAAGCACTCGGCAAAGTCCTGGCGAATCTCGTCATCCCAGTGGTCGTCATCCATGACGCCGCCACCGCCCC
GGCCGTCACCGACGGTCTCGTCGACGGTGGACTTCCCGTCGCCGAGGTCACCTTCCGTACCGACGCCGCACCCGCCGCCA
TCGAGTCGATGGCCAAGGACGGACGCGTCATGGTCGGAGCCGGAACCGTCATCAATGCCCGTCAGGTTGACGAGGCGGTG
AGCGCAGGAGCGACGTTCGTCGTCTCCCCTGGCCTGTCCCGCGAGGTCGTCGACCGCTGCCGTCACCATGACATCCCCGT
CCTGCCGGGAGTCATCACCCCGACCGAGATCATGGCCGGAATCGACATGGGACTGGACACCTTCAAGTTCTTCCCGGCCG
GCAACTTCGGTGGCGCGACCACGATCGCGTCGCTCGATGGCCCCTTCCCCAACCTGCGCTTCGTTCCCACCGGTGGTGTC
AAGCCGCACAACCTGGCGGACTTCCTCTCCCGTCCCAACATTCCTGCCGTCGGAGGCACCTGGCTCACCCCGGCCGACGC
CGTCGCCGCCCGGGACAGCCGAACCATCGCCAACCTGGCACGCGAGGCCGCCCGCAGTGCCCACAGCATCGTCGAGGAGC
AGTCATGA

Upstream 100 bases:

>100_bases
CGCTTTCGACAAAATCGACGGGAAACGATGGCAAGGCCTCGCCACCCCCTCCCCCTCCGGAACAACAATTGACACAAACC
CCCAACGTCGGAGATCACAA

Downstream 100 bases:

>100_bases
GCATCACACTTCGTCCCGCAGATCGGTGCCGTTACGACATCGTCTCCCTTGGTGAGGTGATGTTACGCCTCGACCCACGC
GAACGTCGCATCCGCACCGC

Product: KHG/KDPG aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSNEALGKVLANLVIPVVVIHDAATAPAVTDGLVDGGLPVAEVTFRTDAAPAAIESMAKDGRVMVGAGTVINARQVDEAV
SAGATFVVSPGLSREVVDRCRHHDIPVLPGVITPTEIMAGIDMGLDTFKFFPAGNFGGATTIASLDGPFPNLRFVPTGGV
KPHNLADFLSRPNIPAVGGTWLTPADAVAARDSRTIANLAREAARSAHSIVEEQS

Sequences:

>Translated_215_residues
MSNEALGKVLANLVIPVVVIHDAATAPAVTDGLVDGGLPVAEVTFRTDAAPAAIESMAKDGRVMVGAGTVINARQVDEAV
SAGATFVVSPGLSREVVDRCRHHDIPVLPGVITPTEIMAGIDMGLDTFKFFPAGNFGGATTIASLDGPFPNLRFVPTGGV
KPHNLADFLSRPNIPAVGGTWLTPADAVAARDSRTIANLAREAARSAHSIVEEQS
>Mature_214_residues
SNEALGKVLANLVIPVVVIHDAATAPAVTDGLVDGGLPVAEVTFRTDAAPAAIESMAKDGRVMVGAGTVINARQVDEAVS
AGATFVVSPGLSREVVDRCRHHDIPVLPGVITPTEIMAGIDMGLDTFKFFPAGNFGGATTIASLDGPFPNLRFVPTGGVK
PHNLADFLSRPNIPAVGGTWLTPADAVAARDSRTIANLAREAARSAHSIVEEQS

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=177, Percent_Identity=45.7627118644068, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI48994954, Length=140, Percent_Identity=29.2857142857143, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22167; Mature: 22036

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEALGKVLANLVIPVVVIHDAATAPAVTDGLVDGGLPVAEVTFRTDAAPAAIESMAKD
CCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHC
GRVMVGAGTVINARQVDEAVSAGATFVVSPGLSREVVDRCRHHDIPVLPGVITPTEIMAG
CEEEEECCCEECHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
IDMGLDTFKFFPAGNFGGATTIASLDGPFPNLRFVPTGGVKPHNLADFLSRPNIPAVGGT
HHCCCHHEEECCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCC
WLTPADAVAARDSRTIANLAREAARSAHSIVEEQS
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNEALGKVLANLVIPVVVIHDAATAPAVTDGLVDGGLPVAEVTFRTDAAPAAIESMAKD
CHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHC
GRVMVGAGTVINARQVDEAVSAGATFVVSPGLSREVVDRCRHHDIPVLPGVITPTEIMAG
CEEEEECCCEECHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHH
IDMGLDTFKFFPAGNFGGATTIASLDGPFPNLRFVPTGGVKPHNLADFLSRPNIPAVGGT
HHCCCHHEEECCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCC
WLTPADAVAARDSRTIANLAREAARSAHSIVEEQS
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]