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The map label for this gene is hemD [H]

Identifier: 50841809

GI number: 50841809

Start: 372405

End: 374120

Strand: Direct

Name: hemD [H]

Synonym: PPA0323

Alternate gene names: 50841809

Gene position: 372405-374120 (Clockwise)

Preceding gene: 50841808

Following gene: 50841810

Centisome position: 14.55

GC content: 60.66

Gene sequence:

>1716_bases
ATGACTGACGAGGCTCACCAGCCGACGCCGCAGCCGGCCGGTGATCTGGGCACTGCGTCGTCTCAGGCCAGGGTGCAGTT
TGTGGGTACCGGTCCAGGAGATCCGAATCTGTTGACCCTTGGAGCTGTTGACGCCATCAATCGCGCTCAGGTCATCGTCA
TTTCGTGCGCTGAGCACCGCATGCTGCTGGGCAGTGATTTTCTTGGACTTCGCCCCGACGTCAGAATCGTGTTGGCGGGT
GGTGTTGATGAAGAAGCCGCCGTCCTACCCAGTCAGCCAGGGACTGGCGCTCCGACTCCCGTCGACGTGGACGAACACTG
CGCTGACGTTACTGCCACCGTTATTGATGCCGCCTCCCAAGGCCTTGAAGTCGTTCGTCTCGTTTCTGGGGATCCTTTCC
TTGATGGCGATATTGGCGCGGAGGCTGCCGCTGTGGCTCGCGCTGATTATGACGTCGACGTTGTTCCCGGTGTCACTGGC
ATGACCGCTGTCCCGGAGTACGCCGGACTGACCTTGCATGGCCATGACGTCCAGCTCATCGGGGACGCTGCATGTCAGCG
CGATATTGCTGGACATGGTTCGAACTGGTCAGATCAGGGGCTTGTCGTCGTCAATACCGAGGCAGGCAAGCTTAAAGAAG
TCGTCAAACACGCGATTGAATCTGGACGCGGTGAGGATGAGCCCGCGGCCCTTATCTGCCATGGCGCGACGACCCAGCAG
ACCACTCATACCGTCACCCTCGGTGAGTTACCACAGACCGCTAAGACAGCGCGCCTGGGTGACGCTGAGCCGGTTCACGT
CGCCATCGGCAAAGTTGTCGAAGCCCCCGAACGTGAGCAGCTCGATTGGTACGAGTCCAAGCCGTTATTTGGCTGGAACA
TTCTCATCCCGCGCACCCGTGACCACTCCGCGACCCTGCCCTCGCGACTACAGAGTTACGGTGCTCATTCCCTTGATGTT
CCCACGATTTCGATGGAGCCTCCGCGTACTCCTCAGCAGATGGATAAAGCTATTCGCGGTCTTGTTGAGGGACGTTACGA
GTGGGTCGTCTTTACTTCTGCCAACGCGGTACGTGCCGTTTCGGAGAAGCTAGAGCAGATCGGTCTGGACGCTCGCGCGT
ACTCAGGCTTGCGGATTGCCGCGATCTTCGACTCGACTATCAACGCTTTGGCTGAATGGGGTGTTCGACCCGATTTCGTG
CCATCGGGCGAGCAGTCCACCCGAGCTCTGGCCGCGGTATTCCCGGCCTTTGACGAGGCCCTCGACCCCATTAACCGCGT
TTTCGTGCCGCGTGCCGACATCGCCACTGAGTCCCTGTCGGCCGACCTATCTGAACTGGGATGGGAGGTCGAAGACGTCG
TCGGGTATCGCACCGTGCGAGCTGCCCCGCCGCCAGCTGAGACCCGTGAAGCCATTAAGACCGGCAAATTCGACGCCGTC
GTGTTTACCTCCTCGACGACTGTGCGCAACCTCGTCGGTATTGCCGGAAAACCACATAAGCACACCGTGGTCGCCGCCGT
TGGTATGCGCACCGCTGAAACCTGCCGTGAGCACGGCCTGACCGTCGATGTCATAGCCCCCGAGCCGACTGCGTTGAGCT
TGGCTGACGCCTTGGCGGATTTCGCTGCGAAGCGCCGTGACGACATGGTTGCCAAGGGCCTGCCGGTAGTTCGGCCCTCC
CAGCGCAAGCGTCGTGGCCGTCATCCGTCACGCTGA

Upstream 100 bases:

>100_bases
GCAGGTTTGCGCCTTTCCTCACAGACCAGCACTATTGACCTCGATGCCCGATGCCGGAGCACACCGCCTGGCATGACGAC
AGACACCGGAAGGACTCCAG

Downstream 100 bases:

>100_bases
CACAGCCCCATGACGTAAGGTCATGGGCAGGGCGGGGTTCGCCGCCCGCGAGGTATCGGAAAGGGAGACGATGGGCGCCA
CGACGACTGTCCACCTGCTG

Product: uroporphyrinogen-III synthase, HemD

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MTDEAHQPTPQPAGDLGTASSQARVQFVGTGPGDPNLLTLGAVDAINRAQVIVISCAEHRMLLGSDFLGLRPDVRIVLAG
GVDEEAAVLPSQPGTGAPTPVDVDEHCADVTATVIDAASQGLEVVRLVSGDPFLDGDIGAEAAAVARADYDVDVVPGVTG
MTAVPEYAGLTLHGHDVQLIGDAACQRDIAGHGSNWSDQGLVVVNTEAGKLKEVVKHAIESGRGEDEPAALICHGATTQQ
TTHTVTLGELPQTAKTARLGDAEPVHVAIGKVVEAPEREQLDWYESKPLFGWNILIPRTRDHSATLPSRLQSYGAHSLDV
PTISMEPPRTPQQMDKAIRGLVEGRYEWVVFTSANAVRAVSEKLEQIGLDARAYSGLRIAAIFDSTINALAEWGVRPDFV
PSGEQSTRALAAVFPAFDEALDPINRVFVPRADIATESLSADLSELGWEVEDVVGYRTVRAAPPPAETREAIKTGKFDAV
VFTSSTTVRNLVGIAGKPHKHTVVAAVGMRTAETCREHGLTVDVIAPEPTALSLADALADFAAKRRDDMVAKGLPVVRPS
QRKRRGRHPSR

Sequences:

>Translated_571_residues
MTDEAHQPTPQPAGDLGTASSQARVQFVGTGPGDPNLLTLGAVDAINRAQVIVISCAEHRMLLGSDFLGLRPDVRIVLAG
GVDEEAAVLPSQPGTGAPTPVDVDEHCADVTATVIDAASQGLEVVRLVSGDPFLDGDIGAEAAAVARADYDVDVVPGVTG
MTAVPEYAGLTLHGHDVQLIGDAACQRDIAGHGSNWSDQGLVVVNTEAGKLKEVVKHAIESGRGEDEPAALICHGATTQQ
TTHTVTLGELPQTAKTARLGDAEPVHVAIGKVVEAPEREQLDWYESKPLFGWNILIPRTRDHSATLPSRLQSYGAHSLDV
PTISMEPPRTPQQMDKAIRGLVEGRYEWVVFTSANAVRAVSEKLEQIGLDARAYSGLRIAAIFDSTINALAEWGVRPDFV
PSGEQSTRALAAVFPAFDEALDPINRVFVPRADIATESLSADLSELGWEVEDVVGYRTVRAAPPPAETREAIKTGKFDAV
VFTSSTTVRNLVGIAGKPHKHTVVAAVGMRTAETCREHGLTVDVIAPEPTALSLADALADFAAKRRDDMVAKGLPVVRPS
QRKRRGRHPSR
>Mature_570_residues
TDEAHQPTPQPAGDLGTASSQARVQFVGTGPGDPNLLTLGAVDAINRAQVIVISCAEHRMLLGSDFLGLRPDVRIVLAGG
VDEEAAVLPSQPGTGAPTPVDVDEHCADVTATVIDAASQGLEVVRLVSGDPFLDGDIGAEAAAVARADYDVDVVPGVTGM
TAVPEYAGLTLHGHDVQLIGDAACQRDIAGHGSNWSDQGLVVVNTEAGKLKEVVKHAIESGRGEDEPAALICHGATTQQT
THTVTLGELPQTAKTARLGDAEPVHVAIGKVVEAPEREQLDWYESKPLFGWNILIPRTRDHSATLPSRLQSYGAHSLDVP
TISMEPPRTPQQMDKAIRGLVEGRYEWVVFTSANAVRAVSEKLEQIGLDARAYSGLRIAAIFDSTINALAEWGVRPDFVP
SGEQSTRALAAVFPAFDEALDPINRVFVPRADIATESLSADLSELGWEVEDVVGYRTVRAAPPPAETREAIKTGKFDAVV
FTSSTTVRNLVGIAGKPHKHTVVAAVGMRTAETCREHGLTVDVIAPEPTALSLADALADFAAKRRDDMVAKGLPVVRPSQ
RKRRGRHPSR

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=278, Percent_Identity=26.6187050359712, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 60622; Mature: 60491

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEAHQPTPQPAGDLGTASSQARVQFVGTGPGDPNLLTLGAVDAINRAQVIVISCAEHR
CCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEECCCC
MLLGSDFLGLRPDVRIVLAGGVDEEAAVLPSQPGTGAPTPVDVDEHCADVTATVIDAASQ
EEECCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GLEVVRLVSGDPFLDGDIGAEAAAVARADYDVDVVPGVTGMTAVPEYAGLTLHGHDVQLI
CCEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCHHCCEEEECCCEEEE
GDAACQRDIAGHGSNWSDQGLVVVNTEAGKLKEVVKHAIESGRGEDEPAALICHGATTQQ
EHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
TTHTVTLGELPQTAKTARLGDAEPVHVAIGKVVEAPEREQLDWYESKPLFGWNILIPRTR
CEEEEEECCCCCHHHHHCCCCCCCCEEEHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCC
DHSATLPSRLQSYGAHSLDVPTISMEPPRTPQQMDKAIRGLVEGRYEWVVFTSANAVRAV
CCCCCCHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHH
SEKLEQIGLDARAYSGLRIAAIFDSTINALAEWGVRPDFVPSGEQSTRALAAVFPAFDEA
HHHHHHHCCCHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LDPINRVFVPRADIATESLSADLSELGWEVEDVVGYRTVRAAPPPAETREAIKTGKFDAV
HHHHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHCEEEEECCCCCHHHHHHHHCCCCEEE
VFTSSTTVRNLVGIAGKPHKHTVVAAVGMRTAETCREHGLTVDVIAPEPTALSLADALAD
EEECCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
FAAKRRDDMVAKGLPVVRPSQRKRRGRHPSR
HHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
TDEAHQPTPQPAGDLGTASSQARVQFVGTGPGDPNLLTLGAVDAINRAQVIVISCAEHR
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEECCCC
MLLGSDFLGLRPDVRIVLAGGVDEEAAVLPSQPGTGAPTPVDVDEHCADVTATVIDAASQ
EEECCCCCCCCCCEEEEEECCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GLEVVRLVSGDPFLDGDIGAEAAAVARADYDVDVVPGVTGMTAVPEYAGLTLHGHDVQLI
CCEEEEEECCCCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCHHCCEEEECCCEEEE
GDAACQRDIAGHGSNWSDQGLVVVNTEAGKLKEVVKHAIESGRGEDEPAALICHGATTQQ
EHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCC
TTHTVTLGELPQTAKTARLGDAEPVHVAIGKVVEAPEREQLDWYESKPLFGWNILIPRTR
CEEEEEECCCCCHHHHHCCCCCCCCEEEHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCC
DHSATLPSRLQSYGAHSLDVPTISMEPPRTPQQMDKAIRGLVEGRYEWVVFTSANAVRAV
CCCCCCHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHH
SEKLEQIGLDARAYSGLRIAAIFDSTINALAEWGVRPDFVPSGEQSTRALAAVFPAFDEA
HHHHHHHCCCHHHCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LDPINRVFVPRADIATESLSADLSELGWEVEDVVGYRTVRAAPPPAETREAIKTGKFDAV
HHHHHHEECCCCHHHHHHHHHHHHHHCCCHHHHHCEEEEECCCCCHHHHHHHHCCCCEEE
VFTSSTTVRNLVGIAGKPHKHTVVAAVGMRTAETCREHGLTVDVIAPEPTALSLADALAD
EEECCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
FAAKRRDDMVAKGLPVVRPSQRKRRGRHPSR
HHHHHHHHHHHCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]