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The map label for this gene is sacY [H]

Identifier: 50841781

GI number: 50841781

Start: 341497

End: 342408

Strand: Reverse

Name: sacY [H]

Synonym: PPA0292

Alternate gene names: 50841781

Gene position: 342408-341497 (Counterclockwise)

Preceding gene: 50841782

Following gene: 50841779

Centisome position: 13.37

GC content: 58.99

Gene sequence:

>912_bases
GTGCAACGCCCGGATCGCTCACGGTACGCAACGACGAAGCAGGGATCGCTCAGACCCGGGCACGTCATCGTCAAGAAGAT
TTACAACAACAATGTCCTTCTCGGCGTCAACGGTTCGGGGACCGAAATGGTCGTCAATGCTCGCGGTATCGCCTACGGAC
GACACCGCGGGGAGATCGTCGATGCCTCGTCGGCCCAGCGATATGTCGCAGAGGGTGCCTATCGCACGACCGCCATCGCA
TCACTGCTAACGAACGCCACTCACACCGAGGTGCGAGTGGCACAGGCAATCGTCGAATTGGCGCGCGAAGAGCTGGGCAC
TCCCCATGCCCGACGGATGATGCTGCCCATCCTCGATCACCTCGTCGCAGCTGTGCACCGAGCTAAGCAGGGGGCCGTCA
TCGACTTTCCCTTGGAATGGGAAGTCCGTCAGCTCTATCCCGATGAGGCGGAACTGGGCCGACGCGCTGTCGAAATCGTC
GACGGTGCTCTCGGAATCCATCTGCAACCCGAGGAATGGGTGGCATTCTCCCTGCACTTCATCAATCAGCGGTGGGACAG
TAAAGACGTTTCGCGGACCATGTCGATGACCCAGACGATCTGCGACGTTTTCACCGAGCTGGAGGACCTGTGGCACGTTG
AGATCGACCGTTCGTCCATGAGCGCATCCCGCTTCGTCACCCACCTTCGCTATCTGTTCGCTCGGGCCTCGGACAACAAG
CAGCTCTCTCACGTTGACCTGGACATTGTGGGGCTCATGTCAGATCGCTACCCAGAAGCCACATTGGCAGCTAGCCAAGT
GGCCGAGCACATGTCGAAAGTGATCGGCAACGACCTGACGAAAGCCGAAATCAACTACATCGCCTTACACACCACCCGGC
TCTACAACGAGGTGATGAGGATGGATGGCTGA

Upstream 100 bases:

>100_bases
CTGATCTCCCCCGTGGTCGTGACCAACGCGGCCGGGTTCACCATCTCGGAACGCAGCAATGATCCGGCGTCAGTGCTCTC
AGTCACCGCAGGATGACCCG

Downstream 100 bases:

>100_bases
CGATCGCGCACCTGTTAAGTGCTCATCGCTAGCGGTCAATACACAAAATTGCGATGACCTTCTTGCCGGAAAGCCAGCAC
CAAAGTCACCCAGATCAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQRPDRSRYATTKQGSLRPGHVIVKKIYNNNVLLGVNGSGTEMVVNARGIAYGRHRGEIVDASSAQRYVAEGAYRTTAIA
SLLTNATHTEVRVAQAIVELAREELGTPHARRMMLPILDHLVAAVHRAKQGAVIDFPLEWEVRQLYPDEAELGRRAVEIV
DGALGIHLQPEEWVAFSLHFINQRWDSKDVSRTMSMTQTICDVFTELEDLWHVEIDRSSMSASRFVTHLRYLFARASDNK
QLSHVDLDIVGLMSDRYPEATLAASQVAEHMSKVIGNDLTKAEINYIALHTTRLYNEVMRMDG

Sequences:

>Translated_303_residues
MQRPDRSRYATTKQGSLRPGHVIVKKIYNNNVLLGVNGSGTEMVVNARGIAYGRHRGEIVDASSAQRYVAEGAYRTTAIA
SLLTNATHTEVRVAQAIVELAREELGTPHARRMMLPILDHLVAAVHRAKQGAVIDFPLEWEVRQLYPDEAELGRRAVEIV
DGALGIHLQPEEWVAFSLHFINQRWDSKDVSRTMSMTQTICDVFTELEDLWHVEIDRSSMSASRFVTHLRYLFARASDNK
QLSHVDLDIVGLMSDRYPEATLAASQVAEHMSKVIGNDLTKAEINYIALHTTRLYNEVMRMDG
>Mature_303_residues
MQRPDRSRYATTKQGSLRPGHVIVKKIYNNNVLLGVNGSGTEMVVNARGIAYGRHRGEIVDASSAQRYVAEGAYRTTAIA
SLLTNATHTEVRVAQAIVELAREELGTPHARRMMLPILDHLVAAVHRAKQGAVIDFPLEWEVRQLYPDEAELGRRAVEIV
DGALGIHLQPEEWVAFSLHFINQRWDSKDVSRTMSMTQTICDVFTELEDLWHVEIDRSSMSASRFVTHLRYLFARASDNK
QLSHVDLDIVGLMSDRYPEATLAASQVAEHMSKVIGNDLTKAEINYIALHTTRLYNEVMRMDG

Specific function: In the presence of sucrose, SacY is activated and prevents premature termination of transcription by binding to a RNA-antiterminator (RAT) sequence (partially overlapping with the terminator sequence) located upstream of the sacB gene. Formation of the Sa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PRD domains [H]

Homologues:

Organism=Escherichia coli, GI1790160, Length=275, Percent_Identity=25.4545454545455, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004341
- InterPro:   IPR011608
- InterPro:   IPR001550 [H]

Pfam domain/function: PF03123 CAT_RBD; PF00874 PRD [H]

EC number: NA

Molecular weight: Translated: 34106; Mature: 34106

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRPDRSRYATTKQGSLRPGHVIVKKIYNNNVLLGVNGSGTEMVVNARGIAYGRHRGEIV
CCCCCCCCCCCCCCCCCCCCHHHHEEECCCCEEEEECCCCCEEEEECCCEEECCCCCCEE
DASSAQRYVAEGAYRTTAIASLLTNATHTEVRVAQAIVELAREELGTPHARRMMLPILDH
CCCHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LVAAVHRAKQGAVIDFPLEWEVRQLYPDEAELGRRAVEIVDGALGIHLQPEEWVAFSLHF
HHHHHHHHCCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHH
INQRWDSKDVSRTMSMTQTICDVFTELEDLWHVEIDRSSMSASRFVTHLRYLFARASDNK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
QLSHVDLDIVGLMSDRYPEATLAASQVAEHMSKVIGNDLTKAEINYIALHTTRLYNEVMR
EEEEECEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHH
MDG
CCC
>Mature Secondary Structure
MQRPDRSRYATTKQGSLRPGHVIVKKIYNNNVLLGVNGSGTEMVVNARGIAYGRHRGEIV
CCCCCCCCCCCCCCCCCCCCHHHHEEECCCCEEEEECCCCCEEEEECCCEEECCCCCCEE
DASSAQRYVAEGAYRTTAIASLLTNATHTEVRVAQAIVELAREELGTPHARRMMLPILDH
CCCHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LVAAVHRAKQGAVIDFPLEWEVRQLYPDEAELGRRAVEIVDGALGIHLQPEEWVAFSLHF
HHHHHHHHCCCCEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHH
INQRWDSKDVSRTMSMTQTICDVFTELEDLWHVEIDRSSMSASRFVTHLRYLFARASDNK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHCCCCC
QLSHVDLDIVGLMSDRYPEATLAASQVAEHMSKVIGNDLTKAEINYIALHTTRLYNEVMR
EEEEECEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHH
MDG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2116367; 1806041; 7934828; 9384377; 8535520; 9202047; 9261875; 9305643 [H]