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The map label for this gene is potB [H]

Identifier: 50365327

GI number: 50365327

Start: 602167

End: 603162

Strand: Reverse

Name: potB [H]

Synonym: Mfl510

Alternate gene names: 50365327

Gene position: 603162-602167 (Counterclockwise)

Preceding gene: 50365328

Following gene: 50365326

Centisome position: 76.04

GC content: 27.71

Gene sequence:

>996_bases
ATGAGTAAAAAAGAAAAACAAATTTCAAATACTTTTGATCAAGAACAATTAGACACAGCAATTGATAATCAAATTGCTCG
TGAAAATAAAGTAAAAGTTCGTCAAAAGATTAAAGAAATTAATAAAGTTTTAGGTAAAACTAAATTATTTAACTTTACCA
AAGGTAAGGTTTGACCAATATTATTACCATTTTTTATAGTAATGACTCTATTGGTTATTATTCCTATCATTTCAATTGTT
GTGTATTCTATAGTTCAACCAAGTGGTGACTTAAAAATGTTTGAGATAAGTTTAGAAAAATTTATCAGATTATTTAAAAA
TGGAAATATTATGATGTCAATGGGATTAACAATCGCTTATGCATTTATTGCTTCAGTTATGTGTATTCTTTTAGGATACC
CTATTGCATTAATTATGTCTGAAATGAAATCAAAAATATTGGCTAAAAATATGTGATTATTAGTTACAATGCCAATGTGA
ATTTCAATGTTATTAAAAGTTCTAGGGTTAAGAAGTTTATTTTTAATCATGGCACCAAGTGCTTTAGGAACACAAATAGC
AATTATTATTGGGATGACATATATGTTTATTCCTTTTGCAATAACTCCAATTTATGATTCTTTAGATTCAAGAAGAAGAG
ATATTGAAGAAGCAGCTATGGACTTAGGGTGTTCAAAATATAAAACATTTTGAGGTGTTACTTTAAGATCATCAATGCCT
GGAGTTATTACAGCTATTACTTTAGTATTATTACAAGCTGCAACATCACTTATTGTTGTGCAATACATGGGTAATGGAAA
AATCAATTTAATTACATCAATTATTGAGTCATATTTCTTTAAAGGAAGTGACTTTGGATTTGGAGCTGCGATAAGTGTAG
TATTGGCAGCAATGGTATTCTTATTAATGATGGTCTCTAAATTCTTTACAAATAAATTTGAGAAGAAAGGAGCAAAAGCA
TGAAAAGATTCGTCAGATCAACTTACTTTGCTTTAA

Upstream 100 bases:

>100_bases
AAAATACACACAACTCAAGCATTTAAAGTAGATGACAAAGTATCTATTAAATGAAATGATGAAGCTATTCATGTAATGTG
AAAAGAAGTAGAATAGTTTA

Downstream 100 bases:

>100_bases
TCCTTTTAGTAATATATGTCCCTATTGGAATCATGGTATTATTTTCTTTCAATAGTGGTTCATCAGTTTCAAACTGAATG
GGTTTTAGTACAAGATGATA

Product: spermidine/putrescine ABC transporter permease component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSKKEKQISNTFDQEQLDTAIDNQIARENKVKVRQKIKEINKVLGKTKLFNFTKGKVWPILLPFFIVMTLLVIIPIISIV
VYSIVQPSGDLKMFEISLEKFIRLFKNGNIMMSMGLTIAYAFIASVMCILLGYPIALIMSEMKSKILAKNMWLLVTMPMW
ISMLLKVLGLRSLFLIMAPSALGTQIAIIIGMTYMFIPFAITPIYDSLDSRRRDIEEAAMDLGCSKYKTFWGVTLRSSMP
GVITAITLVLLQAATSLIVVQYMGNGKINLITSIIESYFFKGSDFGFGAAISVVLAAMVFLLMMVSKFFTNKFEKKGAKA
WKDSSDQLTLL

Sequences:

>Translated_331_residues
MSKKEKQISNTFDQEQLDTAIDNQIARENKVKVRQKIKEINKVLGKTKLFNFTKGKV*PILLPFFIVMTLLVIIPIISIV
VYSIVQPSGDLKMFEISLEKFIRLFKNGNIMMSMGLTIAYAFIASVMCILLGYPIALIMSEMKSKILAKNM*LLVTMPM*
ISMLLKVLGLRSLFLIMAPSALGTQIAIIIGMTYMFIPFAITPIYDSLDSRRRDIEEAAMDLGCSKYKTF*GVTLRSSMP
GVITAITLVLLQAATSLIVVQYMGNGKINLITSIIESYFFKGSDFGFGAAISVVLAAMVFLLMMVSKFFTNKFEKKGAKA
*KDSSDQLTLL
>Mature_330_residues
SKKEKQISNTFDQEQLDTAIDNQIARENKVKVRQKIKEINKVLGKTKLFNFTKGKV*PILLPFFIVMTLLVIIPIISIVV
YSIVQPSGDLKMFEISLEKFIRLFKNGNIMMSMGLTIAYAFIASVMCILLGYPIALIMSEMKSKILAKNM*LLVTMPM*I
SMLLKVLGLRSLFLIMAPSALGTQIAIIIGMTYMFIPFAITPIYDSLDSRRRDIEEAAMDLGCSKYKTF*GVTLRSSMPG
VITAITLVLLQAATSLIVVQYMGNGKINLITSIIESYFFKGSDFGFGAAISVVLAAMVFLLMMVSKFFTNKFEKKGAKA*
KDSSDQLTLL

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=213, Percent_Identity=31.4553990610329, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787080, Length=210, Percent_Identity=27.6190476190476, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36160; Mature: 36029

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKEKQISNTFDQEQLDTAIDNQIARENKVKVRQKIKEINKVLGKTKLFNFTKGKVPIL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LPFFIVMTLLVIIPIISIVVYSIVQPSGDLKMFEISLEKFIRLFKNGNIMMSMGLTIAYA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
FIASVMCILLGYPIALIMSEMKSKILAKNMLLVTMPMISMLLKVLGLRSLFLIMAPSALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TQIAIIIGMTYMFIPFAITPIYDSLDSRRRDIEEAAMDLGCSKYKTFGVTLRSSMPGVIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCEEHHCCCCHHHH
AITLVLLQAATSLIVVQYMGNGKINLITSIIESYFFKGSDFGFGAAISVVLAAMVFLLMM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VSKFFTNKFEKKGAKAKDSSDQLTLL
HHHHHHHHHHHCCCCCCCCCCCEECC
>Mature Secondary Structure 
SKKEKQISNTFDQEQLDTAIDNQIARENKVKVRQKIKEINKVLGKTKLFNFTKGKVPIL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LPFFIVMTLLVIIPIISIVVYSIVQPSGDLKMFEISLEKFIRLFKNGNIMMSMGLTIAYA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHH
FIASVMCILLGYPIALIMSEMKSKILAKNMLLVTMPMISMLLKVLGLRSLFLIMAPSALG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TQIAIIIGMTYMFIPFAITPIYDSLDSRRRDIEEAAMDLGCSKYKTFGVTLRSSMPGVIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCEEHHCCCCHHHH
AITLVLLQAATSLIVVQYMGNGKINLITSIIESYFFKGSDFGFGAAISVVLAAMVFLLMM
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VSKFFTNKFEKKGAKAKDSSDQLTLL
HHHHHHHHHHHCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]