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The map label for this gene is rlmH

Identifier: 50365295

GI number: 50365295

Start: 562795

End: 563262

Strand: Reverse

Name: rlmH

Synonym: Mfl478

Alternate gene names: 50365295

Gene position: 563262-562795 (Counterclockwise)

Preceding gene: 50365296

Following gene: 50365294

Centisome position: 71.01

GC content: 20.94

Gene sequence:

>468_bases
ATGAATATCAAAATAGTTTGTTTTGGTAAATTAGATAAGAAATTCTTTATTGATTCATTTAATGAATACGCAAATAGAAT
ATCAAAATATGCTAATTTACAAGTTATTGAACTTAAAGAAGAATACCAAAAAGAAGATGTGGTAAATAAAAATATTAATT
CAGATTTATTAATAGATAAATTAAAAGCATTTTCAGATCATGAAATAATTTGTATGGATGTAAGTTCTAAAAATTATTCA
ACAGAAGAATTTATGTCAATCATTGAAAATAATAAAAATTTAAAGCAAGCAAAAATTGTTTTTGTAATAGGGCCAAGTGA
TGGTTTTAGTGATAAATTTTTACAACAAAATTATAAAAAAGTTTCTTTTGGTAACATCACGTTACCACATCAATTATTTA
GAATTATTTTAGCTGAACAAATATATCGTGCATTTAAAATAATGAATAATGAAAAATATCATAAATAA

Upstream 100 bases:

>100_bases
AAAACAAGTTCTTGAAATGGCTAAAATGATGAAAAGCGGACGTATGCCAGGAATGCCTGGAATGGGTAAAGGAGGATTTG
GTTTTTAATCAAATCTCATC

Downstream 100 bases:

>100_bases
TTTTTTGAATATGTGACACTTAATTGTTATAATAATTAAGTGTAATTATTGGGTTAATACTCAAGTTGGTGAAGAGGACA
CCCTGCTAAGGTGTTAGGTC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MNIKIVCFGKLDKKFFIDSFNEYANRISKYANLQVIELKEEYQKEDVVNKNINSDLLIDKLKAFSDHEIICMDVSSKNYS
TEEFMSIIENNKNLKQAKIVFVIGPSDGFSDKFLQQNYKKVSFGNITLPHQLFRIILAEQIYRAFKIMNNEKYHK

Sequences:

>Translated_155_residues
MNIKIVCFGKLDKKFFIDSFNEYANRISKYANLQVIELKEEYQKEDVVNKNINSDLLIDKLKAFSDHEIICMDVSSKNYS
TEEFMSIIENNKNLKQAKIVFVIGPSDGFSDKFLQQNYKKVSFGNITLPHQLFRIILAEQIYRAFKIMNNEKYHK
>Mature_155_residues
MNIKIVCFGKLDKKFFIDSFNEYANRISKYANLQVIELKEEYQKEDVVNKNINSDLLIDKLKAFSDHEIICMDVSSKNYS
TEEFMSIIENNKNLKQAKIVFVIGPSDGFSDKFLQQNYKKVSFGNITLPHQLFRIILAEQIYRAFKIMNNEKYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=160, Percent_Identity=26.875, Blast_Score=77, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_MESFL (Q6F0Y7)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053720.1
- ProteinModelPortal:   Q6F0Y7
- SMR:   Q6F0Y7
- GeneID:   2897742
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl478
- HOGENOM:   HBG622807
- OMA:   CFGKLDK
- ProtClustDB:   CLSK745770
- BioCyc:   MFLO265311:MFL478-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18249; Mature: 18249

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKIVCFGKLDKKFFIDSFNEYANRISKYANLQVIELKEEYQKEDVVNKNINSDLLIDK
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCHHHHHH
LKAFSDHEIICMDVSSKNYSTEEFMSIIENNKNLKQAKIVFVIGPSDGFSDKFLQQNYKK
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHCCCC
VSFGNITLPHQLFRIILAEQIYRAFKIMNNEKYHK
CCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIKIVCFGKLDKKFFIDSFNEYANRISKYANLQVIELKEEYQKEDVVNKNINSDLLIDK
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCHHHHHH
LKAFSDHEIICMDVSSKNYSTEEFMSIIENNKNLKQAKIVFVIGPSDGFSDKFLQQNYKK
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHCCCC
VSFGNITLPHQLFRIILAEQIYRAFKIMNNEKYHK
CCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA