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The map label for this gene is fmt [H]

Identifier: 50365227

GI number: 50365227

Start: 477395

End: 478336

Strand: Reverse

Name: fmt [H]

Synonym: Mfl409

Alternate gene names: 50365227

Gene position: 478336-477395 (Counterclockwise)

Preceding gene: 50365228

Following gene: 50365226

Centisome position: 60.3

GC content: 27.28

Gene sequence:

>942_bases
ATGGAAAAAATTAAAGTTATATTTTGTGGCACTCCACAAATTGGGGCTGACATTTTAACAACATTAACTGAAATGGAAAA
TGTTGAAGTAGTATTAGTTATTTCTCAACCTGACAGACCTGTTGGCAGAAAAAAAGAATTGAAACCAACTCCTGTTAAAG
AAATTGCTTTAAAAAATAATTTAAAAATAATTCAGCCAGTTAAAATTTCTGAAGCTTATGAAGAAATTGCTCAAATCGAA
AGTGATTTTATTGTTACATGTGCTTATGGTCAATTTGTACCAACAAAAATATTGGATCTGCCAAAAATTGATTCAATTAA
TGTTCATGGAAGTTTGTTACCAAAATATCGTGGTGGTGCACCTATTCAGTATGCTATTAAAAATGGTGATTCTAAAACTG
GTATATCAATTATGAAAATGGTTAAAAAGATGGATGCAGGTGATTATTATATTCAAGAATCAATTGATATTGAAGAAACT
GATGATACAGGAATCATGTTTGAAAAATTAGCTAAACTAGGTCAAAAAATGATTAAAGAAAATTTATTAAAAATTTATAA
TAATGAATTACCGCCAATTGCTCAAAATGAAGATGAAGTAACTTTTTCAAAAAATATTTCAACAGAAGAAGAAAAAATTA
ACTGAAATGATTTATCAGTTAATGTTTGAAATCATATCAGAAGTTTATCACCATGACCAATAGCTCATACATTTAAAGGT
GAAGAAAGATACAAAATTCAAAAAGTACAAATTTTAAATAATAATGTAGAAGCAAAACCAGGAACAATAGTTAATATTAG
TGAAAATGGAATAGACATTCAAACAAAAGATGGTCAAGTTCAAATTTTACTTATTCAAAAACCAGGTAAAAAAATGATGG
AAGCAAGTTCATACAAACTGAACAATCTTTCTGATTTAAAAGTTGGTGATTGTTTTGAGTAA

Upstream 100 bases:

>100_bases
GATTTAAAAGTAAAAGAAGGAATGCTTAAATATTGTGATAGAGATACTCTTGCAATGGTAGTTATTCTTAAAAAAGTGAA
AGAATTAGTGGAGGCACATA

Downstream 100 bases:

>100_bases
AAGATGATCAATTAAATATATATGTTTAATTGGAATGCTAACCGCGTTGCTTGTAACTCTGTCATTAGTAACTATGCTAT
TTACTGCTGATGGTGTTGCT

Product: methyonyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MEKIKVIFCGTPQIGADILTTLTEMENVEVVLVISQPDRPVGRKKELKPTPVKEIALKNNLKIIQPVKISEAYEEIAQIE
SDFIVTCAYGQFVPTKILDLPKIDSINVHGSLLPKYRGGAPIQYAIKNGDSKTGISIMKMVKKMDAGDYYIQESIDIEET
DDTGIMFEKLAKLGQKMIKENLLKIYNNELPPIAQNEDEVTFSKNISTEEEKINWNDLSVNVWNHIRSLSPWPIAHTFKG
EERYKIQKVQILNNNVEAKPGTIVNISENGIDIQTKDGQVQILLIQKPGKKMMEASSYKLNNLSDLKVGDCFE

Sequences:

>Translated_313_residues
MEKIKVIFCGTPQIGADILTTLTEMENVEVVLVISQPDRPVGRKKELKPTPVKEIALKNNLKIIQPVKISEAYEEIAQIE
SDFIVTCAYGQFVPTKILDLPKIDSINVHGSLLPKYRGGAPIQYAIKNGDSKTGISIMKMVKKMDAGDYYIQESIDIEET
DDTGIMFEKLAKLGQKMIKENLLKIYNNELPPIAQNEDEVTFSKNISTEEEKIN*NDLSVNV*NHIRSLSP*PIAHTFKG
EERYKIQKVQILNNNVEAKPGTIVNISENGIDIQTKDGQVQILLIQKPGKKMMEASSYKLNNLSDLKVGDCFE
>Mature_313_residues
MEKIKVIFCGTPQIGADILTTLTEMENVEVVLVISQPDRPVGRKKELKPTPVKEIALKNNLKIIQPVKISEAYEEIAQIE
SDFIVTCAYGQFVPTKILDLPKIDSINVHGSLLPKYRGGAPIQYAIKNGDSKTGISIMKMVKKMDAGDYYIQESIDIEET
DDTGIMFEKLAKLGQKMIKENLLKIYNNELPPIAQNEDEVTFSKNISTEEEKIN*NDLSVNV*NHIRSLSP*PIAHTFKG
EERYKIQKVQILNNNVEAKPGTIVNISENGIDIQTKDGQVQILLIQKPGKKMMEASSYKLNNLSDLKVGDCFE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI238814322, Length=313, Percent_Identity=28.1150159744409, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21614513, Length=226, Percent_Identity=26.5486725663717, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI164663775, Length=182, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789683, Length=296, Percent_Identity=36.8243243243243, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788589, Length=248, Percent_Identity=27.0161290322581, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=326, Percent_Identity=24.2331288343558, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571984, Length=227, Percent_Identity=29.5154185022026, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI45550868, Length=227, Percent_Identity=29.5154185022026, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24585660, Length=318, Percent_Identity=24.2138364779874, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 34652; Mature: 34652

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIKVIFCGTPQIGADILTTLTEMENVEVVLVISQPDRPVGRKKELKPTPVKEIALKNN
CCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCC
LKIIQPVKISEAYEEIAQIESDFIVTCAYGQFVPTKILDLPKIDSINVHGSLLPKYRGGA
CEEEECEEHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
PIQYAIKNGDSKTGISIMKMVKKMDAGDYYIQESIDIEETDDTGIMFEKLAKLGQKMIKE
CEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
NLLKIYNNELPPIAQNEDEVTFSKNISTEEEKINNDLSVNVNHIRSLSPPIAHTFKGEER
HHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHCCCCEEEEHHHHHCCCCCHHHHCCCCCC
YKIQKVQILNNNVEAKPGTIVNISENGIDIQTKDGQVQILLIQKPGKKMMEASSYKLNNL
EEEEEEEEECCCCCCCCCEEEEECCCCEEEEECCCEEEEEEEECCCHHHHHHCCCCCCCC
SDLKVGDCFE
CCCCCCCCCC
>Mature Secondary Structure
MEKIKVIFCGTPQIGADILTTLTEMENVEVVLVISQPDRPVGRKKELKPTPVKEIALKNN
CCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCC
LKIIQPVKISEAYEEIAQIESDFIVTCAYGQFVPTKILDLPKIDSINVHGSLLPKYRGGA
CEEEECEEHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
PIQYAIKNGDSKTGISIMKMVKKMDAGDYYIQESIDIEETDDTGIMFEKLAKLGQKMIKE
CEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
NLLKIYNNELPPIAQNEDEVTFSKNISTEEEKINNDLSVNVNHIRSLSPPIAHTFKGEER
HHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHCCCCEEEEHHHHHCCCCCHHHHCCCCCC
YKIQKVQILNNNVEAKPGTIVNISENGIDIQTKDGQVQILLIQKPGKKMMEASSYKLNNL
EEEEEEEEECCCCCCCCCEEEEECCCCEEEEECCCEEEEEEEECCCHHHHHHCCCCCCCC
SDLKVGDCFE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA