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The map label for this gene is mraW [H]

Identifier: 50365212

GI number: 50365212

Start: 454506

End: 455432

Strand: Reverse

Name: mraW [H]

Synonym: Mfl394

Alternate gene names: 50365212

Gene position: 455432-454506 (Counterclockwise)

Preceding gene: 50365213

Following gene: 50365211

Centisome position: 57.42

GC content: 27.62

Gene sequence:

>927_bases
ATGGAAAAACATATACCAGTATTATTGAAAGAATCAATTGAGTATCTAAATATTAAGGAAAATGGAATTTATGTTGACTG
TACTCTTGGTAGAGCTGGACATTCAAGTGAAATACTAAAAAAATTAAAAGATGGTAAATTATTTTCAATTGATCAAGATG
AAACAGCAATATTAGAAGGAACTGAAAAACTTACAAAAATTAGTAACAATTTCAAAATACTAGAAGGAAATTTCGTTAAC
ATTTCAGCAATGTTGGCAATGCAAGGGATTTTCGAGGTTGATGGTATCCTTTATGATTTAGGAGTTTCTTCGCCACAATT
TGATGTGGCAGAAAGAGGTTTCAGTTATAGATTTGATGGTCCACTTGATATGAGAATGGATAGAGCTAATAATTCTCTAA
CTGCTCACAAAATAGTGAACGAATATACACAAGAAGAACTAGAGCAAATTTTATGAAATTATGGTGATGAAAAATTTGCA
AGATCAATTGCAAAAAATATTATTCTTTCAAGACCTATAAATACAACATTTGAATTAGTTAGTGTAATAAAAAAATCTTT
ACCAGCAAAAATATTGAAACAACAAAAACACCCTGCTAAAAAAACATTTCAAGCTCTAAGAATAAGAGTTAACAATGAAA
TGGAAACTTTAGAAAGTTCGTTAGAACAAAGTTTAAATTTATTAAAACCAAAAGGAAGGGTTGTTGTTATAACTTTCCAT
TCATTAGAAGAAAAAGTTGTTAAAAACATATTTAAGAAATATACACTTGATGAACAACAATTTTACTTAAGTAATTTACC
ATATGAAATTGAATCATCAAAAGACTTTAAACTGTTGTTTAAAAAGCCATTAAAACCCACAAATACAGAAGTAGAAAATA
ATAATAGAAGTCATAGCGCAAAGCTTTGAGTAATTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
TTGAAATATGAGATCAAAAATCATATGACAATTATCAAAATGATAACAGTGACAGAATGGAAGAAATTGCTGAAACAATT
TATCAAGGTTTAAATAAATA

Downstream 100 bases:

>100_bases
AGAAAAAAAGAGGATAGAGTTATGTTAAAAAATAATAAAACATTTACAACAGTAGAAATAAGAAAAAGTGTAGTTAAAAT
TAATGCTTATAGAGTATTAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEKHIPVLLKESIEYLNIKENGIYVDCTLGRAGHSSEILKKLKDGKLFSIDQDETAILEGTEKLTKISNNFKILEGNFVN
ISAMLAMQGIFEVDGILYDLGVSSPQFDVAERGFSYRFDGPLDMRMDRANNSLTAHKIVNEYTQEELEQILWNYGDEKFA
RSIAKNIILSRPINTTFELVSVIKKSLPAKILKQQKHPAKKTFQALRIRVNNEMETLESSLEQSLNLLKPKGRVVVITFH
SLEEKVVKNIFKKYTLDEQQFYLSNLPYEIESSKDFKLLFKKPLKPTNTEVENNNRSHSAKLWVIEKK

Sequences:

>Translated_308_residues
MEKHIPVLLKESIEYLNIKENGIYVDCTLGRAGHSSEILKKLKDGKLFSIDQDETAILEGTEKLTKISNNFKILEGNFVN
ISAMLAMQGIFEVDGILYDLGVSSPQFDVAERGFSYRFDGPLDMRMDRANNSLTAHKIVNEYTQEELEQIL*NYGDEKFA
RSIAKNIILSRPINTTFELVSVIKKSLPAKILKQQKHPAKKTFQALRIRVNNEMETLESSLEQSLNLLKPKGRVVVITFH
SLEEKVVKNIFKKYTLDEQQFYLSNLPYEIESSKDFKLLFKKPLKPTNTEVENNNRSHSAKL*VIEKK
>Mature_308_residues
MEKHIPVLLKESIEYLNIKENGIYVDCTLGRAGHSSEILKKLKDGKLFSIDQDETAILEGTEKLTKISNNFKILEGNFVN
ISAMLAMQGIFEVDGILYDLGVSSPQFDVAERGFSYRFDGPLDMRMDRANNSLTAHKIVNEYTQEELEQIL*NYGDEKFA
RSIAKNIILSRPINTTFELVSVIKKSLPAKILKQQKHPAKKTFQALRIRVNNEMETLESSLEQSLNLLKPKGRVVVITFH
SLEEKVVKNIFKKYTLDEQQFYLSNLPYEIESSKDFKLLFKKPLKPTNTEVENNNRSHSAKL*VIEKK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=331, Percent_Identity=37.1601208459215, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=40, Blast_Score=127, Evalue=9e-30,
Organism=Escherichia coli, GI1786270, Length=315, Percent_Identity=40.3174603174603, Blast_Score=211, Evalue=4e-56,
Organism=Drosophila melanogaster, GI62472493, Length=323, Percent_Identity=35.2941176470588, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28571637, Length=323, Percent_Identity=35.2941176470588, Blast_Score=172, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35024; Mature: 35024

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHIPVLLKESIEYLNIKENGIYVDCTLGRAGHSSEILKKLKDGKLFSIDQDETAILEG
CCCCCCHHHHCCHHHEEEECCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHH
TEKLTKISNNFKILEGNFVNISAMLAMQGIFEVDGILYDLGVSSPQFDVAERGFSYRFDG
HHHHHHHCCCEEEEECCEEEHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCCCEECCC
PLDMRMDRANNSLTAHKIVNEYTQEELEQILNYGDEKFARSIAKNIILSRPINTTFELVS
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VIKKSLPAKILKQQKHPAKKTFQALRIRVNNEMETLESSLEQSLNLLKPKGRVVVITFHS
HHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
LEEKVVKNIFKKYTLDEQQFYLSNLPYEIESSKDFKLLFKKPLKPTNTEVENNNRSHSAK
HHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCEEE
LVIEKK
EEEECC
>Mature Secondary Structure
MEKHIPVLLKESIEYLNIKENGIYVDCTLGRAGHSSEILKKLKDGKLFSIDQDETAILEG
CCCCCCHHHHCCHHHEEEECCCEEEEEECCCCCCHHHHHHHHCCCCEEEECCCCHHHHHH
TEKLTKISNNFKILEGNFVNISAMLAMQGIFEVDGILYDLGVSSPQFDVAERGFSYRFDG
HHHHHHHCCCEEEEECCEEEHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHCCCCEECCC
PLDMRMDRANNSLTAHKIVNEYTQEELEQILNYGDEKFARSIAKNIILSRPINTTFELVS
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
VIKKSLPAKILKQQKHPAKKTFQALRIRVNNEMETLESSLEQSLNLLKPKGRVVVITFHS
HHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
LEEKVVKNIFKKYTLDEQQFYLSNLPYEIESSKDFKLLFKKPLKPTNTEVENNNRSHSAK
HHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCCCEEE
LVIEKK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA