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The map label for this gene is yibK [C]

Identifier: 50365192

GI number: 50365192

Start: 434484

End: 435023

Strand: Reverse

Name: yibK [C]

Synonym: Mfl374

Alternate gene names: 50365192

Gene position: 435023-434484 (Counterclockwise)

Preceding gene: 50365193

Following gene: 50365191

Centisome position: 54.84

GC content: 28.15

Gene sequence:

>540_bases
ATGGAAAGACAAGTTAATATAGTTTTATATGAGCCAGAAATAGCTCAAAACGTAGCAGCAATAATTAGAACTTGTGTTGC
TACAAATGCTAAGTTACATATTATAGAACCTCTAGGTTTTATATTTAAAAAAGAAGAAATGAACAGAGCTAGTGCTGGAA
CTTTTGATTTAGTTGAATACCAACTATATGATGATTGAGAAGATTTTTGTGAAAAAAATCCAAGTATTGATTTATTCTGT
ATTTCAAGATATGGTGAAAAACCACATAGTGATTTTGATTTTAATTCATATCAAAAACCTGTTTTTGTAATGTTTGGTAA
AGAATCTACAGGTATACCAAAACAAATTATTAAAGATAACTATGAAAATACTTTTAGACTTCCAATGTCACCTCAAACTC
GAAGCATTAATGTTGCTAATACAGTAGGGATAGTGACTTATGAAATATTAAGACAATGAGATTATCCAACATTAAGTAAA
ACAGAAGTGCAAAAAGGTAAAGACTATATTATGAGCGAAAGATGAAAAGATGAAAAATAA

Upstream 100 bases:

>100_bases
TATTGTGCAGATTTAACTGAAGAAGATAAAATTAATTGTTCAGCTAGAGCTTATGCGTTGAATGATATTAAAAAAATAAT
TGAAAAGGAAATTAAATAAA

Downstream 100 bases:

>100_bases
TAAAAAAATAGCACTTTTTGGTGGAAGTTTTGACCCTGTACACACTGATCATTTTAATATGGCAAAAACTTGTCATGACA
AACTTGGTTATGAAGAAGTT

Product: rRNA methyltransferase

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MERQVNIVLYEPEIAQNVAAIIRTCVATNAKLHIIEPLGFIFKKEEMNRASAGTFDLVEYQLYDDWEDFCEKNPSIDLFC
ISRYGEKPHSDFDFNSYQKPVFVMFGKESTGIPKQIIKDNYENTFRLPMSPQTRSINVANTVGIVTYEILRQWDYPTLSK
TEVQKGKDYIMSERWKDEK

Sequences:

>Translated_179_residues
MERQVNIVLYEPEIAQNVAAIIRTCVATNAKLHIIEPLGFIFKKEEMNRASAGTFDLVEYQLYDD*EDFCEKNPSIDLFC
ISRYGEKPHSDFDFNSYQKPVFVMFGKESTGIPKQIIKDNYENTFRLPMSPQTRSINVANTVGIVTYEILRQ*DYPTLSK
TEVQKGKDYIMSER*KDEK
>Mature_179_residues
MERQVNIVLYEPEIAQNVAAIIRTCVATNAKLHIIEPLGFIFKKEEMNRASAGTFDLVEYQLYDD*EDFCEKNPSIDLFC
ISRYGEKPHSDFDFNSYQKPVFVMFGKESTGIPKQIIKDNYENTFRLPMSPQTRSINVANTVGIVTYEILRQ*DYPTLSK
TEVQKGKDYIMSER*KDEK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=153, Percent_Identity=39.2156862745098, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20329; Mature: 20329

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERQVNIVLYEPEIAQNVAAIIRTCVATNAKLHIIEPLGFIFKKEEMNRASAGTFDLVEY
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEEE
QLYDDEDFCEKNPSIDLFCISRYGEKPHSDFDFNSYQKPVFVMFGKESTGIPKQIIKDNY
EEECCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCC
ENTFRLPMSPQTRSINVANTVGIVTYEILRQDYPTLSKTEVQKGKDYIMSERKDEK
CCEEECCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MERQVNIVLYEPEIAQNVAAIIRTCVATNAKLHIIEPLGFIFKKEEMNRASAGTFDLVEY
CCCEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCEEEEEE
QLYDDEDFCEKNPSIDLFCISRYGEKPHSDFDFNSYQKPVFVMFGKESTGIPKQIIKDNY
EEECCHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCC
ENTFRLPMSPQTRSINVANTVGIVTYEILRQDYPTLSKTEVQKGKDYIMSERKDEK
CCEEECCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]