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The map label for this gene is nadD [C]

Identifier: 50365191

GI number: 50365191

Start: 433385

End: 434494

Strand: Reverse

Name: nadD [C]

Synonym: Mfl373

Alternate gene names: 50365191

Gene position: 434494-433385 (Counterclockwise)

Preceding gene: 50365192

Following gene: 50365190

Centisome position: 54.78

GC content: 24.5

Gene sequence:

>1110_bases
ATGAAAAATAATAAAAAAATAGCACTTTTTGGTGGAAGTTTTGACCCTGTACACACTGATCATTTTAATATGGCAAAAAC
TTGTCATGACAAACTTGGTTATGAAGAAGTTTGAATAATACCTACATTTTTAAATCCTTTTAAATCGTCTACAAAAACTT
CAAATGAAGAAAGATTAAATTTACTTAATCTTATTTTTGAAGATGAAAATTATATTAAAATTAATCAATTTGAAATGAAC
AATCAAAGAGTTACAACAACTTTTGAAACAGTTTCACATTTCAAAAAAATGTATCCAGAATATGATTTTTCTTTTGTTAT
TGGTTCAGATAATTTAGATAGATTAGAAGAATGAAATAATTTTGACGAACTTATTAATTTAGTTAATTTTATTGTGTTTG
AAAGAACTAATGAATATAAAAAAGATGTAGCAATAAAATATAGTTTACCTATTTATCATTTTGATAACAATTTTTTAAGT
TCAACAAAAATAAGAAATCTTGAAGAGTTGAATTTATTAACACCTAAAGTAAATGATTATATAAATTATCACTTGTTATA
TCTTCAAACAAGAATGAAACCATTTATGGATGAAGAAAGATATCAACATTGCTTGAATGTTGGAGATATGGCCGCTAAAC
TAGCTGAAATTAATAATATAGATCCTCAACAAGCAAAAGTTGCTGGTACATTGCATGATATTACTAAAAGATGATCAGAA
GAAAAACAAGCTTTATACATTAATAAGTATGAAAAAGCATTATTAGTTGAACCAAAACCTGTCTGACACTCATATACAGG
TGCGTTTCATTTAAAATATGATTGATTAATAAGAGATGAAGAAATAATTAATGCAGTTTACAATCACACTGTTGGTTGTG
AAAATATGTCAACATTGGATATGATTGTTTTTTGTGCAGATAAAATTAGTTCAGAACGAGATTATAAAGATGTTGAATAT
TATCGAGAAGAATGTTTTAAAAATTTAAAAAATGGTTTTATTAAATTACTAATAAACCAATATGAAGTTGCAATTAAAAC
TCATGGAATAGATTCTATTGGCGATAAACTTTTAAAAACTTATAACTTTTATGTAAAAGGAGAAAAATAA

Upstream 100 bases:

>100_bases
ATAGTGACTTATGAAATATTAAGACAATGAGATTATCCAACATTAAGTAAAACAGAAGTGCAAAAAGGTAAAGACTATAT
TATGAGCGAAAGATGAAAAG

Downstream 100 bases:

>100_bases
TGAAATTAATTATAGGAGCTATGCATGAAGAATTGCAGGATTCAATTGCTTTTTACAAATTAAATAAAGTTGAAAATGAA
AAGTTTACAATTTATAAAAA

Product: putative nicotinate-nucleotide adenylyltransferase

Products: Deamino-NAD+; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKNNKKIALFGGSFDPVHTDHFNMAKTCHDKLGYEEVWIIPTFLNPFKSSTKTSNEERLNLLNLIFEDENYIKINQFEMN
NQRVTTTFETVSHFKKMYPEYDFSFVIGSDNLDRLEEWNNFDELINLVNFIVFERTNEYKKDVAIKYSLPIYHFDNNFLS
STKIRNLEELNLLTPKVNDYINYHLLYLQTRMKPFMDEERYQHCLNVGDMAAKLAEINNIDPQQAKVAGTLHDITKRWSE
EKQALYINKYEKALLVEPKPVWHSYTGAFHLKYDWLIRDEEIINAVYNHTVGCENMSTLDMIVFCADKISSERDYKDVEY
YREECFKNLKNGFIKLLINQYEVAIKTHGIDSIGDKLLKTYNFYVKGEK

Sequences:

>Translated_369_residues
MKNNKKIALFGGSFDPVHTDHFNMAKTCHDKLGYEEV*IIPTFLNPFKSSTKTSNEERLNLLNLIFEDENYIKINQFEMN
NQRVTTTFETVSHFKKMYPEYDFSFVIGSDNLDRLEE*NNFDELINLVNFIVFERTNEYKKDVAIKYSLPIYHFDNNFLS
STKIRNLEELNLLTPKVNDYINYHLLYLQTRMKPFMDEERYQHCLNVGDMAAKLAEINNIDPQQAKVAGTLHDITKR*SE
EKQALYINKYEKALLVEPKPV*HSYTGAFHLKYD*LIRDEEIINAVYNHTVGCENMSTLDMIVFCADKISSERDYKDVEY
YREECFKNLKNGFIKLLINQYEVAIKTHGIDSIGDKLLKTYNFYVKGEK
>Mature_369_residues
MKNNKKIALFGGSFDPVHTDHFNMAKTCHDKLGYEEV*IIPTFLNPFKSSTKTSNEERLNLLNLIFEDENYIKINQFEMN
NQRVTTTFETVSHFKKMYPEYDFSFVIGSDNLDRLEE*NNFDELINLVNFIVFERTNEYKKDVAIKYSLPIYHFDNNFLS
STKIRNLEELNLLTPKVNDYINYHLLYLQTRMKPFMDEERYQHCLNVGDMAAKLAEINNIDPQQAKVAGTLHDITKR*SE
EKQALYINKYEKALLVEPKPV*HSYTGAFHLKYD*LIRDEEIINAVYNHTVGCENMSTLDMIVFCADKISSERDYKDVEY
YREECFKNLKNGFIKLLINQYEVAIKTHGIDSIGDKLLKTYNFYVKGEK

Specific function: Catalyzes The Reversible Adenylation Of Nicotinate Mononucleotide (Namn) To Nicotinic Acid Adenine Dinucleotide (Naad). [C]

COG id: COG1057

COG function: function code H; Nicotinic acid mononucleotide adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005249
- InterPro:   IPR004820
- InterPro:   IPR003607
- InterPro:   IPR005248
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2 [H]

EC number: 2.7.7.18 [C]

Molecular weight: Translated: 42710; Mature: 42710

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNKKIALFGGSFDPVHTDHFNMAKTCHDKLGYEEVIIPTFLNPFKSSTKTSNEERLNL
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHCHHHCCCCCCCHHHHHH
LNLIFEDENYIKINQFEMNNQRVTTTFETVSHFKKMYPEYDFSFVIGSDNLDRLEENNFD
HHEEECCCCEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCHH
ELINLVNFIVFERTNEYKKDVAIKYSLPIYHFDNNFLSSTKIRNLEELNLLTPKVNDYIN
HHHHHHHHHHHHCCCCHHHCEEEEEECEEEECCCCCCCHHHHCCHHHHHCCCCCHHHHHE
YHLLYLQTRMKPFMDEERYQHCLNVGDMAAKLAEINNIDPQQAKVAGTLHDITKRSEEKQ
EEEEEEEHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
ALYINKYEKALLVEPKPVHSYTGAFHLKYDLIRDEEIINAVYNHTVGCENMSTLDMIVFC
EEEEECCCCEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHH
ADKISSERDYKDVEYYREECFKNLKNGFIKLLINQYEVAIKTHGIDSIGDKLLKTYNFYV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHEEEE
KGEK
ECCC
>Mature Secondary Structure
MKNNKKIALFGGSFDPVHTDHFNMAKTCHDKLGYEEVIIPTFLNPFKSSTKTSNEERLNL
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCEEEHHHHHCHHHCCCCCCCHHHHHH
LNLIFEDENYIKINQFEMNNQRVTTTFETVSHFKKMYPEYDFSFVIGSDNLDRLEENNFD
HHEEECCCCEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCHHHHCCCCHH
ELINLVNFIVFERTNEYKKDVAIKYSLPIYHFDNNFLSSTKIRNLEELNLLTPKVNDYIN
HHHHHHHHHHHHCCCCHHHCEEEEEECEEEECCCCCCCHHHHCCHHHHHCCCCCHHHHHE
YHLLYLQTRMKPFMDEERYQHCLNVGDMAAKLAEINNIDPQQAKVAGTLHDITKRSEEKQ
EEEEEEEHHCCCCCCHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
ALYINKYEKALLVEPKPVHSYTGAFHLKYDLIRDEEIINAVYNHTVGCENMSTLDMIVFC
EEEEECCCCEEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHH
ADKISSERDYKDVEYYREECFKNLKNGFIKLLINQYEVAIKTHGIDSIGDKLLKTYNFYV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHEEEE
KGEK
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: KCl; NH4Cl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1.1 {diphosphate}} 0.37 {NAD+}} 0.0045 {deamido-NAD+}} 0.08 {nicotinate} 0.03 {nicotinate} 0.2 {nicotinamide} 0.5 {ATP}} [C]

Substrates: ATP; H+; Nicotinate D-ribonucleotide [C]

Specific reaction: ATP + H+ + Nicotinate D-ribonucleotide --> Deamino-NAD+ + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NAD+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633; 10954595 [H]