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The map label for this gene is aspS [H]

Identifier: 50365182

GI number: 50365182

Start: 423916

End: 425649

Strand: Reverse

Name: aspS [H]

Synonym: Mfl365

Alternate gene names: 50365182

Gene position: 425649-423916 (Counterclockwise)

Preceding gene: 50365183

Following gene: 50365181

Centisome position: 53.66

GC content: 26.3

Gene sequence:

>1734_bases
ATGAAAAGAACACATAATTGTAATCAATTAAACATTTCAAATGTAAATCAAGAAGTAACATTAAAGGGATGAATTAAAAA
AATTCGTAAAATGGGACAAATTACATTTATTGATTTAAGGGATTTTTATGGTATTACACAAATTGTTGTTGGTGAAAACA
AACAAGAATTAATTAATAATTTAAAACCTGAGTATGTAATTTCAATAACAGGTAAAGTTATTGAACGTAAATCAAAAAAT
GCAGATATACCTACTGGTGAAATTGAAATTGAAGTTAAAAATATTGAATTAATTAATAAAAGTGAATTAACTCCCTTTGT
TATTGAAAATGATGTAGAAGTTTCAGAAGAAACAAGAATGTCATATAGATATTTAGACTTAAGACGTCAAAAGATACAAA
ATAATATGCTTTTAAGAGCAAAAGTTAACAGTATAATTCGTAAAGAATTTGAAGCTGATAATTTTGTTGAAGTTGAAACT
CCATACTTTGGGAAATCAACTCCAGAAGGAGCAAGAGATTTCTTAGTTCCATCAAGATTAAATAAAAATACTTTTTATGC
ATTACCTCAATCTCCCCAATTGTATAAACAATTATTAATGGTTAGTGGATTTGATAAATATTATCAAATTGTTAGATGTT
TTAGAGATGAAGATTTAAGAAATGATCGTCAACCTGAATTTACTCAATTAGATATGGAAATGTCATTTGCATCTGCTACA
GAGGTTCAAGACCAAATTGAAAAGGTAATTAAGAAAATCTTTTTAGAAGTTAAAGGAATTGATTTTAAAGAAAAATTAAT
TAAAATGCCTTTTAGAGAAGCAATAGATTTATATGGTAGTGACAAACCAGATATTAGATTTGATTTAAAAATTAATACTT
TAAATGAAATATTTGATAAAACTCAAATCAAATTATTTGAAAGTTTTAAAGAAAATAAATTATCAATTAGAGGAATTTGT
GTTGAAGAATTATTGAGCAAAAAACAATTAGAAATTTTAACAGAAACTGCAAAACAAAAGAGTTTTAATAATTTAGCATT
TGCTAAATTTGAAAATGGAACTTGAAGTGGTTCAATCGCTTCAAGTTTATCAGATGGTGAAAAACAAGCTTTAATTAAAC
AATTTAATATTAAAGATAAAGCAACTATTCTACTAAACGTTGGTAAATATGAGAAGATATCAGATATGCTTGGTGCAGTT
AGAAATAAAGTTGCTGAAATTTTAAACTTAGCTGATCCTAATGATTATAAATTATTATGAATTATTGATTTCCCATTATA
TGAATGAAGTGATGAGGAATCTAGATATGTAGCTGCTCATAACCCTTTTACAATGCCAAACATTAAATCAATTGATGATT
TTGAAACTAATAAAGAAGATGCAATAGCAGATTCATATGATTTAGTTTTAAATGGATTTGAACTTGGAAGTGGTGGAGTT
CGTATTACAGACTCAGGAATTCAACAAAGAATGTTTGAAGCTGTAGGATTGGATGATGAAACTATTGAGAAAAACTTTGG
TTGATTTATAAATGCATATAAATATGGAGCACCAAATCATGCTGGTTTTGCTTTTGGAATTGATAGAGTAATCATGTTAC
TTACTCATTCAGAATCAATTAGAGACGTTATAGCTTTCCCTAAAAATTCAAAAGGGATTGATATGATGAATGATGCACCT
AGTTATGTTGAAGACAATCAATTAAGTGAATTAAGTATTAAAACAATAAAATAA

Upstream 100 bases:

>100_bases
TTAATAGTGATATTTATGCAATAAAAGATCAAAAATCTGGTGAAACTAAAGAATTGAAATTTAAAGATATTGTTAAATAC
TTAAAAGGGGAAAAATAGAA

Downstream 100 bases:

>100_bases
GACTAAACATTCATTGTTTAGTTTTTTATTCTGTGCTCACTATTTAAACTAAAAACAAATAAAAATAAAAAATGATAAAA
TTATTTAAAGGGGAAATGAC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MKRTHNCNQLNISNVNQEVTLKGWIKKIRKMGQITFIDLRDFYGITQIVVGENKQELINNLKPEYVISITGKVIERKSKN
ADIPTGEIEIEVKNIELINKSELTPFVIENDVEVSEETRMSYRYLDLRRQKIQNNMLLRAKVNSIIRKEFEADNFVEVET
PYFGKSTPEGARDFLVPSRLNKNTFYALPQSPQLYKQLLMVSGFDKYYQIVRCFRDEDLRNDRQPEFTQLDMEMSFASAT
EVQDQIEKVIKKIFLEVKGIDFKEKLIKMPFREAIDLYGSDKPDIRFDLKINTLNEIFDKTQIKLFESFKENKLSIRGIC
VEELLSKKQLEILTETAKQKSFNNLAFAKFENGTWSGSIASSLSDGEKQALIKQFNIKDKATILLNVGKYEKISDMLGAV
RNKVAEILNLADPNDYKLLWIIDFPLYEWSDEESRYVAAHNPFTMPNIKSIDDFETNKEDAIADSYDLVLNGFELGSGGV
RITDSGIQQRMFEAVGLDDETIEKNFGWFINAYKYGAPNHAGFAFGIDRVIMLLTHSESIRDVIAFPKNSKGIDMMNDAP
SYVEDNQLSELSIKTIK

Sequences:

>Translated_577_residues
MKRTHNCNQLNISNVNQEVTLKG*IKKIRKMGQITFIDLRDFYGITQIVVGENKQELINNLKPEYVISITGKVIERKSKN
ADIPTGEIEIEVKNIELINKSELTPFVIENDVEVSEETRMSYRYLDLRRQKIQNNMLLRAKVNSIIRKEFEADNFVEVET
PYFGKSTPEGARDFLVPSRLNKNTFYALPQSPQLYKQLLMVSGFDKYYQIVRCFRDEDLRNDRQPEFTQLDMEMSFASAT
EVQDQIEKVIKKIFLEVKGIDFKEKLIKMPFREAIDLYGSDKPDIRFDLKINTLNEIFDKTQIKLFESFKENKLSIRGIC
VEELLSKKQLEILTETAKQKSFNNLAFAKFENGT*SGSIASSLSDGEKQALIKQFNIKDKATILLNVGKYEKISDMLGAV
RNKVAEILNLADPNDYKLL*IIDFPLYE*SDEESRYVAAHNPFTMPNIKSIDDFETNKEDAIADSYDLVLNGFELGSGGV
RITDSGIQQRMFEAVGLDDETIEKNFG*FINAYKYGAPNHAGFAFGIDRVIMLLTHSESIRDVIAFPKNSKGIDMMNDAP
SYVEDNQLSELSIKTIK
>Mature_577_residues
MKRTHNCNQLNISNVNQEVTLKG*IKKIRKMGQITFIDLRDFYGITQIVVGENKQELINNLKPEYVISITGKVIERKSKN
ADIPTGEIEIEVKNIELINKSELTPFVIENDVEVSEETRMSYRYLDLRRQKIQNNMLLRAKVNSIIRKEFEADNFVEVET
PYFGKSTPEGARDFLVPSRLNKNTFYALPQSPQLYKQLLMVSGFDKYYQIVRCFRDEDLRNDRQPEFTQLDMEMSFASAT
EVQDQIEKVIKKIFLEVKGIDFKEKLIKMPFREAIDLYGSDKPDIRFDLKINTLNEIFDKTQIKLFESFKENKLSIRGIC
VEELLSKKQLEILTETAKQKSFNNLAFAKFENGT*SGSIASSLSDGEKQALIKQFNIKDKATILLNVGKYEKISDMLGAV
RNKVAEILNLADPNDYKLL*IIDFPLYE*SDEESRYVAAHNPFTMPNIKSIDDFETNKEDAIADSYDLVLNGFELGSGGV
RITDSGIQQRMFEAVGLDDETIEKNFG*FINAYKYGAPNHAGFAFGIDRVIMLLTHSESIRDVIAFPKNSKGIDMMNDAP
SYVEDNQLSELSIKTIK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=596, Percent_Identity=37.4161073825503, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI45439306, Length=266, Percent_Identity=26.6917293233083, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI5031815, Length=215, Percent_Identity=29.3023255813954, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194272210, Length=215, Percent_Identity=29.3023255813954, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788173, Length=587, Percent_Identity=40.0340715502555, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1790571, Length=302, Percent_Identity=27.8145695364238, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789256, Length=301, Percent_Identity=27.5747508305648, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=595, Percent_Identity=35.4621848739496, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI71994340, Length=197, Percent_Identity=26.3959390862944, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=197, Percent_Identity=26.3959390862944, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=147, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=273, Percent_Identity=24.5421245421245, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=633, Percent_Identity=34.2812006319115, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6323011, Length=269, Percent_Identity=28.6245353159851, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=200, Percent_Identity=26.5, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=598, Percent_Identity=34.1137123745819, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17136276, Length=239, Percent_Identity=29.7071129707113, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24640849, Length=237, Percent_Identity=28.2700421940928, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24640851, Length=237, Percent_Identity=28.2700421940928, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 65425; Mature: 65425

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTHNCNQLNISNVNQEVTLKGIKKIRKMGQITFIDLRDFYGITQIVVGENKQELINNL
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCEEEEECCCHHHHHHCC
KPEYVISITGKVIERKSKNADIPTGEIEIEVKNIELINKSELTPFVIENDVEVSEETRMS
CCCEEEEECHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCCCCHHHHHH
YRYLDLRRQKIQNNMLLRAKVNSIIRKEFEADNFVEVETPYFGKSTPEGARDFLVPSRLN
HHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHCCCCCCCC
KNTFYALPQSPQLYKQLLMVSGFDKYYQIVRCFRDEDLRNDRQPEFTQLDMEMSFASATE
CCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHH
VQDQIEKVIKKIFLEVKGIDFKEKLIKMPFREAIDLYGSDKPDIRFDLKINTLNEIFDKT
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHHH
QIKLFESFKENKLSIRGICVEELLSKKQLEILTETAKQKSFNNLAFAKFENGTSGSIASS
HHHHHHHHHHCCEEEEEEEHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
LSDGEKQALIKQFNIKDKATILLNVGKYEKISDMLGAVRNKVAEILNLADPNDYKLLIID
HCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
FPLYESDEESRYVAAHNPFTMPNIKSIDDFETNKEDAIADSYDLVLNGFELGSGGVRITD
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCHHHEEECEEECCCCEEEEC
SGIQQRMFEAVGLDDETIEKNFGFINAYKYGAPNHAGFAFGIDRVIMLLTHSESIRDVIA
HHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHC
FPKNSKGIDMMNDAPSYVEDNQLSELSIKTIK
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MKRTHNCNQLNISNVNQEVTLKGIKKIRKMGQITFIDLRDFYGITQIVVGENKQELINNL
CCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHHCCCEEEEECCCHHHHHHCC
KPEYVISITGKVIERKSKNADIPTGEIEIEVKNIELINKSELTPFVIENDVEVSEETRMS
CCCEEEEECHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCCCCHHHHHH
YRYLDLRRQKIQNNMLLRAKVNSIIRKEFEADNFVEVETPYFGKSTPEGARDFLVPSRLN
HHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHCCCCCCCC
KNTFYALPQSPQLYKQLLMVSGFDKYYQIVRCFRDEDLRNDRQPEFTQLDMEMSFASATE
CCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHH
VQDQIEKVIKKIFLEVKGIDFKEKLIKMPFREAIDLYGSDKPDIRFDLKINTLNEIFDKT
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHHH
QIKLFESFKENKLSIRGICVEELLSKKQLEILTETAKQKSFNNLAFAKFENGTSGSIASS
HHHHHHHHHHCCEEEEEEEHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
LSDGEKQALIKQFNIKDKATILLNVGKYEKISDMLGAVRNKVAEILNLADPNDYKLLIID
HCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
FPLYESDEESRYVAAHNPFTMPNIKSIDDFETNKEDAIADSYDLVLNGFELGSGGVRITD
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCCHHHEEECEEECCCCEEEEC
SGIQQRMFEAVGLDDETIEKNFGFINAYKYGAPNHAGFAFGIDRVIMLLTHSESIRDVIA
HHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHC
FPKNSKGIDMMNDAPSYVEDNQLSELSIKTIK
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA