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The map label for this gene is guaB [H]

Identifier: 50365159

GI number: 50365159

Start: 398692

End: 399834

Strand: Direct

Name: guaB [H]

Synonym: Mfl343

Alternate gene names: 50365159

Gene position: 398692-399834 (Clockwise)

Preceding gene: 50365158

Following gene: 50365160

Centisome position: 50.26

GC content: 31.85

Gene sequence:

>1143_bases
ATGAGAATAAATATGAATAAAGATTTAAATGGAAAAATTATAAATGAAGCAATTACTTTTGATGATGTACTATTAGTACC
TAATTACTCAGAAGTTTTACCTCATGAAGTTTGTTTAAAAACTAAATTAACTAAAAATATAGAATTAAATATTCCTATCA
TTTCTGCTGCAATGGATACAGTTACTGAATCTGAGTTAGCAATTGCAATTGCAAGTATTGGTGGTATTGGTATTGTTCAT
AAAAATTTAACAATTGAACAACAAGCAAATGAAATTAAAATAGTTAAATCAATTAAACCTACAGATGAATTTCCAAATGC
TTGTGTTGATGCTAATGGATTTTTAAGAGTTGGTGGTGCTGTTGGAGTTAATGATGAAACTCTAACACGTGTAGAAGGTC
TAATTTCAGCTGGTATTGATGTTTTAGTAGTTGATTCAGCACATGGTCATAGTAAAGGTATTATTGATGTTGTAAAAGCA
ATTAGAGTTAAATATCCTAACTTAGATATTATTGCTGGTAACATTTGTACAGTTGAAGGTGCTGAAGCTCTTTATAAAGC
TGGAGCTGATTGTGTTAAAGTTGGAATCGGTCCTGGAAGTATTTGTACAACTAGAGTGGTTGCTGGAGTTGGAGTACCTC
AAATTACAGCAATTAATGATGTTTACAATTGATCTATTAATAAAGACGTAACTTTGATTGCAGATGGAGGAATTAAATAC
TCTGGTGATGTTGTTAAAGCTTTAGCAGCTGGTGCTCATTCTGTAATGTTAGGAAGTATGCTAGCTGGAACTGAAGAAGC
ACCTGGTCAAGAAGTTATTATTAATAATAAAAGATATAAAACTTATGTTGGCATGGGTTCTTTAGCAGCAATGAAACGTG
GAAGTAGTGATCGTTACTTCCAAAAGGGAGCTAAAAAATTAGTTCCTGAAGGAATCGAAGCTGTTGTTCCTTTCAAAGGG
ACTTTAGAAGAAGTCATTTTTCAATTAGTTGGTGGTTTAAGAAGTGGAATGGGTTACACTGGTTCTAATACAATAGAAAC
TTTAAGACACAATGCTAAATTTGTTAAAATAACTGGAGCTAGTTTAAAAGAATCTCATCCTCATGATGTAGAAATTTCAG
CTGAAGCTCCAAATTATAAATAA

Upstream 100 bases:

>100_bases
GAGAATTTTTAGATGAAGTAGTAAATGAAATAATAAATAAAGTAGATAATGTTAACCGAGTAGTTTATGATGTTACATCT
AAACCTCCAGGAACAATAGA

Downstream 100 bases:

>100_bases
GAAAATAACTCACTTTTAAAACAAAGTGAGTTTTTATTATTATGTTTTTCTATTAATAATATAAAATATTAGTATCTTTA
AGGCGGATACTATATGATTG

Product: inositol-5-monophosphate dehydrogenase

Products: NA

Alternate protein names: IMP dehydrogenase; IMPD; IMPDH [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MRINMNKDLNGKIINEAITFDDVLLVPNYSEVLPHEVCLKTKLTKNIELNIPIISAAMDTVTESELAIAIASIGGIGIVH
KNLTIEQQANEIKIVKSIKPTDEFPNACVDANGFLRVGGAVGVNDETLTRVEGLISAGIDVLVVDSAHGHSKGIIDVVKA
IRVKYPNLDIIAGNICTVEGAEALYKAGADCVKVGIGPGSICTTRVVAGVGVPQITAINDVYNWSINKDVTLIADGGIKY
SGDVVKALAAGAHSVMLGSMLAGTEEAPGQEVIINNKRYKTYVGMGSLAAMKRGSSDRYFQKGAKKLVPEGIEAVVPFKG
TLEEVIFQLVGGLRSGMGYTGSNTIETLRHNAKFVKITGASLKESHPHDVEISAEAPNYK

Sequences:

>Translated_380_residues
MRINMNKDLNGKIINEAITFDDVLLVPNYSEVLPHEVCLKTKLTKNIELNIPIISAAMDTVTESELAIAIASIGGIGIVH
KNLTIEQQANEIKIVKSIKPTDEFPNACVDANGFLRVGGAVGVNDETLTRVEGLISAGIDVLVVDSAHGHSKGIIDVVKA
IRVKYPNLDIIAGNICTVEGAEALYKAGADCVKVGIGPGSICTTRVVAGVGVPQITAINDVYN*SINKDVTLIADGGIKY
SGDVVKALAAGAHSVMLGSMLAGTEEAPGQEVIINNKRYKTYVGMGSLAAMKRGSSDRYFQKGAKKLVPEGIEAVVPFKG
TLEEVIFQLVGGLRSGMGYTGSNTIETLRHNAKFVKITGASLKESHPHDVEISAEAPNYK
>Mature_380_residues
MRINMNKDLNGKIINEAITFDDVLLVPNYSEVLPHEVCLKTKLTKNIELNIPIISAAMDTVTESELAIAIASIGGIGIVH
KNLTIEQQANEIKIVKSIKPTDEFPNACVDANGFLRVGGAVGVNDETLTRVEGLISAGIDVLVVDSAHGHSKGIIDVVKA
IRVKYPNLDIIAGNICTVEGAEALYKAGADCVKVGIGPGSICTTRVVAGVGVPQITAINDVYN*SINKDVTLIADGGIKY
SGDVVKALAAGAHSVMLGSMLAGTEEAPGQEVIINNKRYKTYVGMGSLAAMKRGSSDRYFQKGAKKLVPEGIEAVVPFKG
TLEEVIFQLVGGLRSGMGYTGSNTIETLRHNAKFVKITGASLKESHPHDVEISAEAPNYK

Specific function: GMP biosynthesis from IMP; first step. [C]

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family [H]

Homologues:

Organism=Homo sapiens, GI217035152, Length=320, Percent_Identity=36.5625, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI34328930, Length=320, Percent_Identity=36.5625, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI34328928, Length=320, Percent_Identity=36.5625, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI217035146, Length=254, Percent_Identity=40.9448818897638, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI156616279, Length=320, Percent_Identity=36.5625, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI217035150, Length=254, Percent_Identity=40.9448818897638, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI217035148, Length=254, Percent_Identity=40.9448818897638, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI50541954, Length=348, Percent_Identity=34.7701149425287, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI50541952, Length=348, Percent_Identity=34.7701149425287, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI50541948, Length=348, Percent_Identity=34.7701149425287, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI50541956, Length=348, Percent_Identity=34.7701149425287, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI66933016, Length=344, Percent_Identity=34.593023255814, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI156104880, Length=348, Percent_Identity=34.1954022988506, Blast_Score=175, Evalue=6e-44,
Organism=Escherichia coli, GI1788855, Length=306, Percent_Identity=54.5751633986928, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1786293, Length=345, Percent_Identity=33.0434782608696, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17560440, Length=359, Percent_Identity=32.033426183844, Blast_Score=178, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71994385, Length=251, Percent_Identity=37.8486055776892, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71994389, Length=201, Percent_Identity=41.7910447761194, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323585, Length=245, Percent_Identity=38.7755102040816, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6323464, Length=249, Percent_Identity=38.5542168674699, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322012, Length=284, Percent_Identity=35.2112676056338, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6319352, Length=231, Percent_Identity=32.9004329004329, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319353, Length=120, Percent_Identity=36.6666666666667, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24641071, Length=380, Percent_Identity=33.6842105263158, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24641073, Length=380, Percent_Identity=33.6842105263158, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI28571163, Length=377, Percent_Identity=33.9522546419098, Blast_Score=185, Evalue=4e-47,

Paralogues:

None

Copy number: 600 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015875
- InterPro:   IPR001093 [H]

Pfam domain/function: PF00478 IMPDH [H]

EC number: =1.1.1.205 [H]

Molecular weight: Translated: 40074; Mature: 40074

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINMNKDLNGKIINEAITFDDVLLVPNYSEVLPHEVCLKTKLTKNIELNIPIISAAMDT
CEECCCCCCCCEEECCEECCCCEEECCCHHHHCCHHHHHHHHCCCCEEEECEEEHHHHHH
VTESELAIAIASIGGIGIVHKNLTIEQQANEIKIVKSIKPTDEFPNACVDANGFLRVGGA
HCHHHHEEEEECCCCEEEEECCEEEECCCCEEEEEECCCCCCCCCCCEECCCCEEEECCE
VGVNDETLTRVEGLISAGIDVLVVDSAHGHSKGIIDVVKAIRVKYPNLDIIAGNICTVEG
ECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCEEEECC
AEALYKAGADCVKVGIGPGSICTTRVVAGVGVPQITAINDVYNSINKDVTLIADGGIKYS
HHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCEEHHHHHHHHCCCCEEEEECCCEEEC
GDVVKALAAGAHSVMLGSMLAGTEEAPGQEVIINNKRYKTYVGMGSLAAMKRGSSDRYFQ
CHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEECCCHHHHHHCCCCCHHHH
KGAKKLVPEGIEAVVPFKGTLEEVIFQLVGGLRSGMGYTGSNTIETLRHNAKFVKITGAS
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCC
LKESHPHDVEISAEAPNYK
CCCCCCCCEEEECCCCCCC
>Mature Secondary Structure
MRINMNKDLNGKIINEAITFDDVLLVPNYSEVLPHEVCLKTKLTKNIELNIPIISAAMDT
CEECCCCCCCCEEECCEECCCCEEECCCHHHHCCHHHHHHHHCCCCEEEECEEEHHHHHH
VTESELAIAIASIGGIGIVHKNLTIEQQANEIKIVKSIKPTDEFPNACVDANGFLRVGGA
HCHHHHEEEEECCCCEEEEECCEEEECCCCEEEEEECCCCCCCCCCCEECCCCEEEECCE
VGVNDETLTRVEGLISAGIDVLVVDSAHGHSKGIIDVVKAIRVKYPNLDIIAGNICTVEG
ECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCEEEECC
AEALYKAGADCVKVGIGPGSICTTRVVAGVGVPQITAINDVYNSINKDVTLIADGGIKYS
HHHHHHCCCCEEEECCCCCCHHHHHHHHCCCCCCEEHHHHHHHHCCCCEEEEECCCEEEC
GDVVKALAAGAHSVMLGSMLAGTEEAPGQEVIINNKRYKTYVGMGSLAAMKRGSSDRYFQ
CHHHHHHHHCHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEECCCHHHHHHCCCCCHHHH
KGAKKLVPEGIEAVVPFKGTLEEVIFQLVGGLRSGMGYTGSNTIETLRHNAKFVKITGAS
HHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEEEEECCC
LKESHPHDVEISAEAPNYK
CCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961392; 9403685; 10758003 [H]