Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 50365108

GI number: 50365108

Start: 336904

End: 338319

Strand: Direct

Name: nusA [H]

Synonym: Mfl292

Alternate gene names: 50365108

Gene position: 336904-338319 (Clockwise)

Preceding gene: 50365107

Following gene: 50365109

Centisome position: 42.47

GC content: 29.59

Gene sequence:

>1416_bases
ATGGTAAACGGAGCACAAATATTAGAAGCATTAGCTTCATTAGAAAGTGAAAAAAGCATTAGCAAAGAAGTTGCTATTGA
AGGAATTAAAGAAGGATTCCAAAAGGCTTATGAAAGATTTTTTGATACAGAAGCTGTAGTTAAAACTGAAATTAATGAAC
AAACAGGATCAATCAACTTGTTTCAAGAATTAATGGTAGTAGCAACAGATGATGAAATCGAAGATGATTGATTAGAAATT
GTTTTAGAAGATGCTTTAAAAATCAATAAAGAAGCAAAAGTTGGAGATAAAGTATACAAACCAATTGATTTTGATGAAGA
ATTCTCAAGAGTAGCTGTTGGACAAGTAAGACAAATCTTCCAACAAAAAATTAGAGCTACTGAAAGAGCTATGATTTATG
AAAAATTTATTCAATTAGAAGGTGAAATTGTTAGAGGTAAAATCGTTGGAATGAACGATCAAGGAACTTCATACATTTTA
GATATTGATGGAGTACATACTTCATTATGAAACCAAAAAACAATCAACAGAGAAGAATTTGTTGTTAACGAATTAGTTGA
TGTTTTATTAGAAGAAGTTGCTAGAGAAAATAAATACTCACAATTAGTTGTTTCAAGAGTAGCACCTAAATTTTTAGCTA
AATTAGTTGAAAAAGAAGTAAGTGAAGTTGCTCAAGGATTAGTTGAAGTAATGTCAGTTTCAAGAGAACCAGGTAAACGT
GCAAAAATTGCTGTTTTATCACATGATGAAGATGTAGAACCAATTGGAGCTATTGTTGGAGTTAAAGGTTCAAGAATCAA
TAACATTTCAAAAGAATTAAGAGGAGAAAAAATTGATGTTGTTAAATGAGATGATGAAATCATTCCATTTATTATTAACG
CAATGGCACCTGTTAAGGTTATTTCTGTAAATGAAGTTGAAGGTGAATTTGATATAGTAGTTCCTAATCAACAATTATCA
TTAGCTATTGGTAAAGGTGGAATGGCTGCTAAATTAGTTGCTAACTTATTAAAACGTCGTATTAACATTTACAGCTTAGA
AAATGCTATTACTGACAACATGGATGTTTTATGAAATGGAAACATTACAGAAGAAGAAGTAAACAACCCAGACTTTATTA
ACGAAGTAAACAAACGTAAAATCAACTCTCAAACAGTTAAACCAGAATACCACAAAAATTCATTTAGAAATGCACAAGCA
AATAATGAAGAAGAATTAATGAGTTTCCAAGCTGAAGTTGAGGAAGAATACAACCAAGTTGAAGAATTAATTGAAGAAAC
AAATGCAGTTGTTCAAGAAAATAAAGATTTAGAGTCAATTCAATCTGAACTAGAATCATTTAATGATATTTTAAATGATG
AAGATGAAGAAGATTTTGAAGAAGACGAATACGAAGATTTATATGATCAAGAATAA

Upstream 100 bases:

>100_bases
TAGAATCACCTACTTTTTAAAAGGTCAAAAGAAAAAAGTTGACTTTAAATATGAACAAGTAAAGTTTGCAAGATATGCAG
TTAAATTTTAAGGAGAAAAT

Downstream 100 bases:

>100_bases
TCTAAGAAAAGATGTCGTTAGTAAAGAAATGTTAGATAAATCAGCATTAATTAGAGTTGTTTTAAATAAAAACAATGAAA
TTTTTATTGATTTAACTTAT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MVNGAQILEALASLESEKSISKEVAIEGIKEGFQKAYERFFDTEAVVKTEINEQTGSINLFQELMVVATDDEIEDDWLEI
VLEDALKINKEAKVGDKVYKPIDFDEEFSRVAVGQVRQIFQQKIRATERAMIYEKFIQLEGEIVRGKIVGMNDQGTSYIL
DIDGVHTSLWNQKTINREEFVVNELVDVLLEEVARENKYSQLVVSRVAPKFLAKLVEKEVSEVAQGLVEVMSVSREPGKR
AKIAVLSHDEDVEPIGAIVGVKGSRINNISKELRGEKIDVVKWDDEIIPFIINAMAPVKVISVNEVEGEFDIVVPNQQLS
LAIGKGGMAAKLVANLLKRRINIYSLENAITDNMDVLWNGNITEEEVNNPDFINEVNKRKINSQTVKPEYHKNSFRNAQA
NNEEELMSFQAEVEEEYNQVEELIEETNAVVQENKDLESIQSELESFNDILNDEDEEDFEEDEYEDLYDQE

Sequences:

>Translated_471_residues
MVNGAQILEALASLESEKSISKEVAIEGIKEGFQKAYERFFDTEAVVKTEINEQTGSINLFQELMVVATDDEIEDD*LEI
VLEDALKINKEAKVGDKVYKPIDFDEEFSRVAVGQVRQIFQQKIRATERAMIYEKFIQLEGEIVRGKIVGMNDQGTSYIL
DIDGVHTSL*NQKTINREEFVVNELVDVLLEEVARENKYSQLVVSRVAPKFLAKLVEKEVSEVAQGLVEVMSVSREPGKR
AKIAVLSHDEDVEPIGAIVGVKGSRINNISKELRGEKIDVVK*DDEIIPFIINAMAPVKVISVNEVEGEFDIVVPNQQLS
LAIGKGGMAAKLVANLLKRRINIYSLENAITDNMDVL*NGNITEEEVNNPDFINEVNKRKINSQTVKPEYHKNSFRNAQA
NNEEELMSFQAEVEEEYNQVEELIEETNAVVQENKDLESIQSELESFNDILNDEDEEDFEEDEYEDLYDQE
>Mature_471_residues
MVNGAQILEALASLESEKSISKEVAIEGIKEGFQKAYERFFDTEAVVKTEINEQTGSINLFQELMVVATDDEIEDD*LEI
VLEDALKINKEAKVGDKVYKPIDFDEEFSRVAVGQVRQIFQQKIRATERAMIYEKFIQLEGEIVRGKIVGMNDQGTSYIL
DIDGVHTSL*NQKTINREEFVVNELVDVLLEEVARENKYSQLVVSRVAPKFLAKLVEKEVSEVAQGLVEVMSVSREPGKR
AKIAVLSHDEDVEPIGAIVGVKGSRINNISKELRGEKIDVVK*DDEIIPFIINAMAPVKVISVNEVEGEFDIVVPNQQLS
LAIGKGGMAAKLVANLLKRRINIYSLENAITDNMDVL*NGNITEEEVNNPDFINEVNKRKINSQTVKPEYHKNSFRNAQA
NNEEELMSFQAEVEEEYNQVEELIEETNAVVQENKDLESIQSELESFNDILNDEDEEDFEEDEYEDLYDQE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=343, Percent_Identity=32.3615160349854, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 52729; Mature: 52729

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNGAQILEALASLESEKSISKEVAIEGIKEGFQKAYERFFDTEAVVKTEINEQTGSINL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHH
FQELMVVATDDEIEDDLEIVLEDALKINKEAKVGDKVYKPIDFDEEFSRVAVGQVRQIFQ
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCHHHCCCCCCCHHHHHHHHHHHHHHHH
QKIRATERAMIYEKFIQLEGEIVRGKIVGMNDQGTSYILDIDGVHTSLNQKTINREEFVV
HHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHHH
NELVDVLLEEVARENKYSQLVVSRVAPKFLAKLVEKEVSEVAQGLVEVMSVSREPGKRAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IAVLSHDEDVEPIGAIVGVKGSRINNISKELRGEKIDVVKDDEIIPFIINAMAPVKVISV
EEEECCCCCCCHHHHHHCCCCCHHHHHHHHHCCCEECEEECCCHHHHHHHCCCCEEEEEE
NEVEGEFDIVVPNQQLSLAIGKGGMAAKLVANLLKRRINIYSLENAITDNMDVLNGNITE
EECCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCH
EEVNNPDFINEVNKRKINSQTVKPEYHKNSFRNAQANNEEELMSFQAEVEEEYNQVEELI
HHCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EETNAVVQENKDLESIQSELESFNDILNDEDEEDFEEDEYEDLYDQE
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure
MVNGAQILEALASLESEKSISKEVAIEGIKEGFQKAYERFFDTEAVVKTEINEQTGSINL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHH
FQELMVVATDDEIEDDLEIVLEDALKINKEAKVGDKVYKPIDFDEEFSRVAVGQVRQIFQ
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHCCHHHCCCCCCCHHHHHHHHHHHHHHHH
QKIRATERAMIYEKFIQLEGEIVRGKIVGMNDQGTSYILDIDGVHTSLNQKTINREEFVV
HHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEEECCCHHHHHHHHCCHHHHHH
NELVDVLLEEVARENKYSQLVVSRVAPKFLAKLVEKEVSEVAQGLVEVMSVSREPGKRAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
IAVLSHDEDVEPIGAIVGVKGSRINNISKELRGEKIDVVKDDEIIPFIINAMAPVKVISV
EEEECCCCCCCHHHHHHCCCCCHHHHHHHHHCCCEECEEECCCHHHHHHHCCCCEEEEEE
NEVEGEFDIVVPNQQLSLAIGKGGMAAKLVANLLKRRINIYSLENAITDNMDVLNGNITE
EECCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCH
EEVNNPDFINEVNKRKINSQTVKPEYHKNSFRNAQANNEEELMSFQAEVEEEYNQVEELI
HHCCCCHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
EETNAVVQENKDLESIQSELESFNDILNDEDEEDFEEDEYEDLYDQE
HHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]