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The map label for this gene is hup2 [H]

Identifier: 50365017

GI number: 50365017

Start: 227763

End: 228035

Strand: Direct

Name: hup2 [H]

Synonym: Mfl201

Alternate gene names: 50365017

Gene position: 227763-228035 (Clockwise)

Preceding gene: 50365016

Following gene: 50365018

Centisome position: 28.71

GC content: 29.3

Gene sequence:

>273_bases
ATGACAAAAAAAGACTTAATTAACGAAATTATTGCTAATGAAAACATCTCAAAAGTTGAATGTGAAGCAGTAGTTAATTC
ATTATTCGATTTAATTATTGAAGAATTATCATCAGGAAACGAAGTTTCAATCGCAGGTTTTGGTAAATTTGCAATTAGTG
AAAGAGCAGCAAGAGAAGGAATCAACCCATCAACAGGTGATAAAATTTCAATCGCAGCTTCTAAATCAGCTAAATTCAAA
GCAGCTAAACAATTAAAAGAAAAATTAAACTAA

Upstream 100 bases:

>100_bases
TAATATATAATATTTTATATAATAATGTTAAGCCTATTTCACTTTTAAATAATGTCTTTAGAGACGTGAAATTAGTTTAA
AAAATTAAAGGAGAAATTAT

Downstream 100 bases:

>100_bases
AAGTAAGCATATGCTTACTTTTTTTTATTTTTAAATTAAAATAAATAACATATGACAAAAAAAGGAGCGTAGCCATGCTT
ATTGAATTATTAGCAAAAGG

Product: histone-like DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MTKKDLINEIIANENISKVECEAVVNSLFDLIIEELSSGNEVSIAGFGKFAISERAAREGINPSTGDKISIAASKSAKFK
AAKQLKEKLN

Sequences:

>Translated_90_residues
MTKKDLINEIIANENISKVECEAVVNSLFDLIIEELSSGNEVSIAGFGKFAISERAAREGINPSTGDKISIAASKSAKFK
AAKQLKEKLN
>Mature_89_residues
TKKDLINEIIANENISKVECEAVVNSLFDLIIEELSSGNEVSIAGFGKFAISERAAREGINPSTGDKISIAASKSAKFKA
AKQLKEKLN

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=43.3333333333333, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=32.967032967033, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1790433, Length=87, Percent_Identity=39.0804597701149, Blast_Score=64, Evalue=3e-12,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9684; Mature: 9553

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKDLINEIIANENISKVECEAVVNSLFDLIIEELSSGNEVSIAGFGKFAISERAAREG
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCC
INPSTGDKISIAASKSAKFKAAKQLKEKLN
CCCCCCCEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKKDLINEIIANENISKVECEAVVNSLFDLIIEELSSGNEVSIAGFGKFAISERAAREG
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCC
INPSTGDKISIAASKSAKFKAAKQLKEKLN
CCCCCCCEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]