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The map label for this gene is trpS [H]

Identifier: 50365008

GI number: 50365008

Start: 219318

End: 220370

Strand: Reverse

Name: trpS [H]

Synonym: Mfl192

Alternate gene names: 50365008

Gene position: 220370-219318 (Counterclockwise)

Preceding gene: 50365019

Following gene: 50365002

Centisome position: 27.78

GC content: 27.54

Gene sequence:

>1053_bases
ATGTTTGATATAATATTATATGTAAAAGTGAGGAAGATAAGAATGCAAAAAGAAAGAATGATAACTGGGATAACACCAAG
TGATTCAATGAGTTTAGGAAACTATTTAGGTGTTGTTAAAAATTTAATTGAGTATCAAAATGAATATGATTTATTTGTTT
TTGTTGCAAATTTACATGCAATAACAATACCAAAAGATCCAAATCAGTTAAAAATTAAAACAAAGGAAATGACAGCATTG
TACATTGCTTGTGGATTAGACCCTGAAAAGATGACTCTTTTCTTACAATCTGATGTTGTTGAACATTCTCAATTAGGTTG
AATTCTTACAACTCAATCAACAATGGGTGAATTATCAAGAATGACACAATTCAAAGATAAATCAGCTAAAGAAAGTAATG
GTGACAATGTTTCTATTCCAGCAGGTTTATTTACTTATCCTTGTTTAATGGCTGCTGATATATTACTTTATGATCCAAAA
TTTGTTCCTGTTGGTGTTGATCAAAAACAACACTTAGAGTTAGCTAGAGATATAGCAACAAGAATGAACAATAAGTACGG
TGAAATGCACGTTGTACCAGAACCTATTTTAACAAAAGGTAATATTAAAATTATGGACTTGCAAGACCCAACCAAAAAAA
TGAGTAAGTCTAGTGAAAATCCTAAAGCTGTTATTAAAATGCTTGATTCACCAGCTGAAATTGCTAATAAAATTAAATCA
GCAGTTACTGATAGTGAAAATTTAATAAAATATGACCCAATTAATAAACCAGGTGTTTCAAATTTAATGAATATTTATTC
AATAATCAAAAACGTTTCAATAGAAGAATGCCAAAAAAAATGAGATGGTAAAAATTATAAAGACTTAAAAGACGATGTTA
CAGAAGCATTGTTAGAAGTAATAACTCCAATTCAGGAAAAATACAATGAAATAATAAAAGGTGATTATATAAAAGAAGTG
CTTGAACTAGGTTCATTAAAAGCTAAAAAAGTTGCTATTAAAAAAGTAAATAAAGTACAAAATAAATTAGGTATTAATTG
ATATAGAAAATAA

Upstream 100 bases:

>100_bases
GACATTTTTTTTGATTCATTATAATCATTTTTTACAATACTATATTTCATTTTGCACCTCAAGTTAATGTAAAATAATTA
TATCAATTATTAGGTTATAT

Downstream 100 bases:

>100_bases
ACTTCACGGTTTATTTTTTTTTATATAAAAATATAAAAAAAGATGCCTAAGCATCTAATTATATTATTCAGGTGCCATTC
AACCAACCATTTTAATTCTT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MFDIILYVKVRKIRMQKERMITGITPSDSMSLGNYLGVVKNLIEYQNEYDLFVFVANLHAITIPKDPNQLKIKTKEMTAL
YIACGLDPEKMTLFLQSDVVEHSQLGWILTTQSTMGELSRMTQFKDKSAKESNGDNVSIPAGLFTYPCLMAADILLYDPK
FVPVGVDQKQHLELARDIATRMNNKYGEMHVVPEPILTKGNIKIMDLQDPTKKMSKSSENPKAVIKMLDSPAEIANKIKS
AVTDSENLIKYDPINKPGVSNLMNIYSIIKNVSIEECQKKWDGKNYKDLKDDVTEALLEVITPIQEKYNEIIKGDYIKEV
LELGSLKAKKVAIKKVNKVQNKLGINWYRK

Sequences:

>Translated_350_residues
MFDIILYVKVRKIRMQKERMITGITPSDSMSLGNYLGVVKNLIEYQNEYDLFVFVANLHAITIPKDPNQLKIKTKEMTAL
YIACGLDPEKMTLFLQSDVVEHSQLG*ILTTQSTMGELSRMTQFKDKSAKESNGDNVSIPAGLFTYPCLMAADILLYDPK
FVPVGVDQKQHLELARDIATRMNNKYGEMHVVPEPILTKGNIKIMDLQDPTKKMSKSSENPKAVIKMLDSPAEIANKIKS
AVTDSENLIKYDPINKPGVSNLMNIYSIIKNVSIEECQKK*DGKNYKDLKDDVTEALLEVITPIQEKYNEIIKGDYIKEV
LELGSLKAKKVAIKKVNKVQNKLGIN*YRK
>Mature_350_residues
MFDIILYVKVRKIRMQKERMITGITPSDSMSLGNYLGVVKNLIEYQNEYDLFVFVANLHAITIPKDPNQLKIKTKEMTAL
YIACGLDPEKMTLFLQSDVVEHSQLG*ILTTQSTMGELSRMTQFKDKSAKESNGDNVSIPAGLFTYPCLMAADILLYDPK
FVPVGVDQKQHLELARDIATRMNNKYGEMHVVPEPILTKGNIKIMDLQDPTKKMSKSSENPKAVIKMLDSPAEIANKIKS
AVTDSENLIKYDPINKPGVSNLMNIYSIIKNVSIEECQKK*DGKNYKDLKDDVTEALLEVITPIQEKYNEIIKGDYIKEV
LELGSLKAKKVAIKKVNKVQNKLGIN*YRK

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=331, Percent_Identity=40.4833836858006, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI41352700, Length=183, Percent_Identity=45.9016393442623, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1789786, Length=334, Percent_Identity=45.2095808383234, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71982800, Length=332, Percent_Identity=35.8433734939759, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71982793, Length=332, Percent_Identity=35.8433734939759, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320474, Length=340, Percent_Identity=39.4117647058824, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24666151, Length=335, Percent_Identity=40.8955223880597, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 39128; Mature: 39128

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDIILYVKVRKIRMQKERMITGITPSDSMSLGNYLGVVKNLIEYQNEYDLFVFVANLHA
CCEEEEEEHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEE
ITIPKDPNQLKIKTKEMTALYIACGLDPEKMTLFLQSDVVEHSQLGILTTQSTMGELSRM
EECCCCCCCEEEEEHHEEEEEEEECCCHHHEEEEEHHHHHHHHHCCEEEEHHHHHHHHHH
TQFKDKSAKESNGDNVSIPAGLFTYPCLMAADILLYDPKFVPVGVDQKQHLELARDIATR
HHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHEECCEEEECCCCHHHHHHHHHHHHHH
MNNKYGEMHVVPEPILTKGNIKIMDLQDPTKKMSKSSENPKAVIKMLDSPAEIANKIKSA
HCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHH
VTDSENLIKYDPINKPGVSNLMNIYSIIKNVSIEECQKKDGKNYKDLKDDVTEALLEVIT
HCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
PIQEKYNEIIKGDYIKEVLELGSLKAKKVAIKKVNKVQNKLGINYRK
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFDIILYVKVRKIRMQKERMITGITPSDSMSLGNYLGVVKNLIEYQNEYDLFVFVANLHA
CCEEEEEEHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCEE
ITIPKDPNQLKIKTKEMTALYIACGLDPEKMTLFLQSDVVEHSQLGILTTQSTMGELSRM
EECCCCCCCEEEEEHHEEEEEEEECCCHHHEEEEEHHHHHHHHHCCEEEEHHHHHHHHHH
TQFKDKSAKESNGDNVSIPAGLFTYPCLMAADILLYDPKFVPVGVDQKQHLELARDIATR
HHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHEECCEEEECCCCHHHHHHHHHHHHHH
MNNKYGEMHVVPEPILTKGNIKIMDLQDPTKKMSKSSENPKAVIKMLDSPAEIANKIKSA
HCCCCCCEEECCCCCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHH
VTDSENLIKYDPINKPGVSNLMNIYSIIKNVSIEECQKKDGKNYKDLKDDVTEALLEVIT
HCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
PIQEKYNEIIKGDYIKEVLELGSLKAKKVAIKKVNKVQNKLGINYRK
HHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]