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The map label for this gene is fruA [H]

Identifier: 50364997

GI number: 50364997

Start: 210162

End: 212309

Strand: Direct

Name: fruA [H]

Synonym: Mfl181

Alternate gene names: 50364997

Gene position: 210162-212309 (Clockwise)

Preceding gene: 50364996

Following gene: 50364998

Centisome position: 26.49

GC content: 31.75

Gene sequence:

>2148_bases
ATGGAATTAAAAGATTTATTTAAATCTAAAGTTTCAGTCTTCAAAGCAGATTTAAAAACAAAAGAAGAAGTTATTAATTT
TTTAGTTGATAAATTATCATCAGAAAAAATGATAGCTAATAAAAAAACTTTTAAAGATGCGATTTTAAAAAGAGAAAGCG
AAGCATCAACAGGTATGGGTGATGGAATTGGTATTCCTCATGCAATTAACAACACAGTTAAAGAACCTTGTATTGCTTTT
GTAAGTTTAAAATCACCAATAGACTGACAAAGTTTAGATAACAAACCAGTAGATTTAATCTTTATGATTGCTACAAATGA
TGAAAAAGGAGAAGCTCACTTAGGTGCTTTAGCTGATTTATCAAAATTCTTAATGAAACCTGAATTTCAAGAAGCATTAA
GAAAAGCAAAAACTTTTAAAGATCTTTCAAAAGCTTTTGATAATAAAGTTGAAACAAAAAAAGTGGAAGCAAAAAATGGT
AAATATGATGTGATTGGTATTACAGCATGTCCAACTGGTATTGCTCACACATACTTAGCAGAAGAAAAATTAATTGAATA
TGCTACAGAATTAGGATTAAGTGTAAAAATTGAAACTCAAGGACGTAGAGGAACTGAAAACAAATTAACTCAAGAAGATG
TTGACAATGCAAAAGTAATTATTTTAGCACATGACAAAAACTTACAAGGAATGGGTAGATTTGGTGGTAAACAAGTAATT
GATACAACTACTAAAGATGCAATTTTTAATGGAAAACAATTAATTCAAGAATTTGGTAAAACAGAAAAAACTACTACAGC
AAAAGCTGTATCTTCAAAAGATGATGAAGTAAGTGATGATTTTTCATTGAAAAAATTTGCTCAAGTTAAAGGAAACTTAC
TTGCGGGTGTTTCAAGAATGTTACCATTTGTCGTTGCAGGAGGAATTATATTAGGAATTGGATTCTTGATTGATTTTGCT
GCAGGAAACGGAAATGTTCCAGGAATGACTGGCGCAACATTTGATTATTGATTTAATAATATATATGACAAAACTAAAGT
TGATGTATTAACAGAATGAACCAATTGATGAATGGGAAATTTTGGAACACATAGTGAAGTTGCTGGTTGATTCTCAGCAA
TAGGTAAAACAGGTATGATGATGATGGTTCCTGTTTTAGCAGCATATATTTCATATACAATCGTTGGACCTCAAGGATTA
ATGCCTGGGTTTATAGCAGGTATATTAGCTGATGGTACAGGTGGTTTTGCATATGCTGGAGACAAATTTGGGTGATCAGG
TTTATGAACACGACTAATACCAGCTGATATCCCTATGCAATCAGGATTTATTGGTGGTATGGTTGGTGCATATGTAGCAG
CCTTAATTGTATTTGGTTTAACAATAGGATTCAAAAAATTTAAAAAATCTTTCCATGGAGTAAGAGACATAGTATTAATT
CCAGTGTTATCTCTTTTAGGTATTTCTTTAGCAATGTTCGCATTAAATATACCTTTAGGATATACAATGTATGGATTACA
ACAATTTTTAAAATTGTTAGCTGATCAAAACTTATTAATATTATTAGGAGCAATCCTTGGATTAATGATGTGTATTGATA
TGGGTGGACCAATTAATAAAATTGCTTATGTTACAGGAACACTTTCAGTTTCAGGTGGATTAGGTAATGATCCACTTATA
ACTGTTACTATGGCAGCAGCAATGGCTGGAGGAATGATTCCACCTTTAGGAATAGCATTATGTACAGTAACTTTTAGAAA
AGCTTGAACAACAAAAGAAAAAGATTCAGCAAAAGCTAACTGATTAATGGGTGCATTCTTTATTTCAGAAGGAGCAATAC
CATTTATGGTTACAGATCCAAAAAGAATTTCAGTTTCAGCAATGGCTGGTGGTACAATCACCGGATTAATTGTTGGAGGA
TGTAAAATAACATTAGGTGCACCACACGGTGGTATAGCTGTATTCCCATTATTAAAATCAGGATTACTTGGAACAAGCCC
TGAACAAATGAATTCAGGAGCAGCAATTGGTTTAGGTGTAGGATTATACATCTTAGCAATCGTTGTTGGTACTTTAGTTA
TGGCAACAATCTTAGGATTCTGAAAAACTTATGATATTAAAAAAGGTAAACTAGTTATTGCCAATTAA

Upstream 100 bases:

>100_bases
AGGTGGGGCAACTGCCTTTACCGAATGATTAGGATTAAAAGAAGACATTTTAAAATTAAAACAAGAAATTAAAGTTAACA
AAATAAAATAGGAGATAAAT

Downstream 100 bases:

>100_bases
TATTAAATAAAAACCTTATTTATTTAAGGTTTTTTTGTTTCCAAAAATATATAAATTTTTTTTCAAGGAGTAAAATAATT
AATAAATGAGAGAAAAACAA

Product: fructose-specific PTS system IIABC component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MELKDLFKSKVSVFKADLKTKEEVINFLVDKLSSEKMIANKKTFKDAILKRESEASTGMGDGIGIPHAINNTVKEPCIAF
VSLKSPIDWQSLDNKPVDLIFMIATNDEKGEAHLGALADLSKFLMKPEFQEALRKAKTFKDLSKAFDNKVETKKVEAKNG
KYDVIGITACPTGIAHTYLAEEKLIEYATELGLSVKIETQGRRGTENKLTQEDVDNAKVIILAHDKNLQGMGRFGGKQVI
DTTTKDAIFNGKQLIQEFGKTEKTTTAKAVSSKDDEVSDDFSLKKFAQVKGNLLAGVSRMLPFVVAGGIILGIGFLIDFA
AGNGNVPGMTGATFDYWFNNIYDKTKVDVLTEWTNWWMGNFGTHSEVAGWFSAIGKTGMMMMVPVLAAYISYTIVGPQGL
MPGFIAGILADGTGGFAYAGDKFGWSGLWTRLIPADIPMQSGFIGGMVGAYVAALIVFGLTIGFKKFKKSFHGVRDIVLI
PVLSLLGISLAMFALNIPLGYTMYGLQQFLKLLADQNLLILLGAILGLMMCIDMGGPINKIAYVTGTLSVSGGLGNDPLI
TVTMAAAMAGGMIPPLGIALCTVTFRKAWTTKEKDSAKANWLMGAFFISEGAIPFMVTDPKRISVSAMAGGTITGLIVGG
CKITLGAPHGGIAVFPLLKSGLLGTSPEQMNSGAAIGLGVGLYILAIVVGTLVMATILGFWKTYDIKKGKLVIAN

Sequences:

>Translated_715_residues
MELKDLFKSKVSVFKADLKTKEEVINFLVDKLSSEKMIANKKTFKDAILKRESEASTGMGDGIGIPHAINNTVKEPCIAF
VSLKSPID*QSLDNKPVDLIFMIATNDEKGEAHLGALADLSKFLMKPEFQEALRKAKTFKDLSKAFDNKVETKKVEAKNG
KYDVIGITACPTGIAHTYLAEEKLIEYATELGLSVKIETQGRRGTENKLTQEDVDNAKVIILAHDKNLQGMGRFGGKQVI
DTTTKDAIFNGKQLIQEFGKTEKTTTAKAVSSKDDEVSDDFSLKKFAQVKGNLLAGVSRMLPFVVAGGIILGIGFLIDFA
AGNGNVPGMTGATFDY*FNNIYDKTKVDVLTE*TN**MGNFGTHSEVAG*FSAIGKTGMMMMVPVLAAYISYTIVGPQGL
MPGFIAGILADGTGGFAYAGDKFG*SGL*TRLIPADIPMQSGFIGGMVGAYVAALIVFGLTIGFKKFKKSFHGVRDIVLI
PVLSLLGISLAMFALNIPLGYTMYGLQQFLKLLADQNLLILLGAILGLMMCIDMGGPINKIAYVTGTLSVSGGLGNDPLI
TVTMAAAMAGGMIPPLGIALCTVTFRKA*TTKEKDSAKAN*LMGAFFISEGAIPFMVTDPKRISVSAMAGGTITGLIVGG
CKITLGAPHGGIAVFPLLKSGLLGTSPEQMNSGAAIGLGVGLYILAIVVGTLVMATILGF*KTYDIKKGKLVIAN
>Mature_715_residues
MELKDLFKSKVSVFKADLKTKEEVINFLVDKLSSEKMIANKKTFKDAILKRESEASTGMGDGIGIPHAINNTVKEPCIAF
VSLKSPID*QSLDNKPVDLIFMIATNDEKGEAHLGALADLSKFLMKPEFQEALRKAKTFKDLSKAFDNKVETKKVEAKNG
KYDVIGITACPTGIAHTYLAEEKLIEYATELGLSVKIETQGRRGTENKLTQEDVDNAKVIILAHDKNLQGMGRFGGKQVI
DTTTKDAIFNGKQLIQEFGKTEKTTTAKAVSSKDDEVSDDFSLKKFAQVKGNLLAGVSRMLPFVVAGGIILGIGFLIDFA
AGNGNVPGMTGATFDY*FNNIYDKTKVDVLTE*TN**MGNFGTHSEVAG*FSAIGKTGMMMMVPVLAAYISYTIVGPQGL
MPGFIAGILADGTGGFAYAGDKFG*SGL*TRLIPADIPMQSGFIGGMVGAYVAALIVFGLTIGFKKFKKSFHGVRDIVLI
PVLSLLGISLAMFALNIPLGYTMYGLQQFLKLLADQNLLILLGAILGLMMCIDMGGPINKIAYVTGTLSVSGGLGNDPLI
TVTMAAAMAGGMIPPLGIALCTVTFRKA*TTKEKDSAKAN*LMGAFFISEGAIPFMVTDPKRISVSAMAGGTITGLIVGG
CKITLGAPHGGIAVFPLLKSGLLGTSPEQMNSGAAIGLGVGLYILAIVVGTLVMATILGF*KTYDIKKGKLVIAN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786951, Length=709, Percent_Identity=28.2087447108604, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1788492, Length=574, Percent_Identity=34.4947735191638, Blast_Score=232, Evalue=7e-62,
Organism=Escherichia coli, GI87082348, Length=490, Percent_Identity=29.5918367346939, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1790386, Length=371, Percent_Identity=32.3450134770889, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790387, Length=85, Percent_Identity=45.8823529411765, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1788729, Length=462, Percent_Identity=25.1082251082251, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788730, Length=94, Percent_Identity=41.4893617021277, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1790390, Length=71, Percent_Identity=45.0704225352113, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 74360; Mature: 74360

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKDLFKSKVSVFKADLKTKEEVINFLVDKLSSEKMIANKKTFKDAILKRESEASTGMG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DGIGIPHAINNTVKEPCIAFVSLKSPIDQSLDNKPVDLIFMIATNDEKGEAHLGALADLS
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
KFLMKPEFQEALRKAKTFKDLSKAFDNKVETKKVEAKNGKYDVIGITACPTGIAHTYLAE
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCEEEEEEEECCCHHHHHHHHH
EKLIEYATELGLSVKIETQGRRGTENKLTQEDVDNAKVIILAHDKNLQGMGRFGGKQVID
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCCEEC
TTTKDAIFNGKQLIQEFGKTEKTTTAKAVSSKDDEVSDDFSLKKFAQVKGNLLAGVSRML
CCHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PFVVAGGIILGIGFLIDFAAGNGNVPGMTGATFDYFNNIYDKTKVDVLTETNMGNFGTHS
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCH
EVAGFSAIGKTGMMMMVPVLAAYISYTIVGPQGLMPGFIAGILADGTGGFAYAGDKFGSG
HHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCEECCCHHCCC
LTRLIPADIPMQSGFIGGMVGAYVAALIVFGLTIGFKKFKKSFHGVRDIVLIPVLSLLGI
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAMFALNIPLGYTMYGLQQFLKLLADQNLLILLGAILGLMMCIDMGGPINKIAYVTGTL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
SVSGGLGNDPLITVTMAAAMAGGMIPPLGIALCTVTFRKATTKEKDSAKANLMGAFFISE
EECCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECC
GAIPFMVTDPKRISVSAMAGGTITGLIVGGCKITLGAPHGGIAVFPLLKSGLLGTSPEQM
CCEEEEEECCCEEEEEEECCCCEEEEEEECEEEEEECCCCCEEHHHHHHCCCCCCCHHHH
NSGAAIGLGVGLYILAIVVGTLVMATILGFKTYDIKKGKLVIAN
CCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEC
>Mature Secondary Structure
MELKDLFKSKVSVFKADLKTKEEVINFLVDKLSSEKMIANKKTFKDAILKRESEASTGMG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCC
DGIGIPHAINNTVKEPCIAFVSLKSPIDQSLDNKPVDLIFMIATNDEKGEAHLGALADLS
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
KFLMKPEFQEALRKAKTFKDLSKAFDNKVETKKVEAKNGKYDVIGITACPTGIAHTYLAE
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCEEEEEEEECCCHHHHHHHHH
EKLIEYATELGLSVKIETQGRRGTENKLTQEDVDNAKVIILAHDKNLQGMGRFGGKQVID
HHHHHHHHHCCCEEEEEECCCCCCCCCCCHHCCCCCEEEEEEECCCCCCCCCCCCCCEEC
TTTKDAIFNGKQLIQEFGKTEKTTTAKAVSSKDDEVSDDFSLKKFAQVKGNLLAGVSRML
CCHHHHHCCHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PFVVAGGIILGIGFLIDFAAGNGNVPGMTGATFDYFNNIYDKTKVDVLTETNMGNFGTHS
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCH
EVAGFSAIGKTGMMMMVPVLAAYISYTIVGPQGLMPGFIAGILADGTGGFAYAGDKFGSG
HHHHHHHHCCCCHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHCCCCCCCEECCCHHCCC
LTRLIPADIPMQSGFIGGMVGAYVAALIVFGLTIGFKKFKKSFHGVRDIVLIPVLSLLGI
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAMFALNIPLGYTMYGLQQFLKLLADQNLLILLGAILGLMMCIDMGGPINKIAYVTGTL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEE
SVSGGLGNDPLITVTMAAAMAGGMIPPLGIALCTVTFRKATTKEKDSAKANLMGAFFISE
EECCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEECC
GAIPFMVTDPKRISVSAMAGGTITGLIVGGCKITLGAPHGGIAVFPLLKSGLLGTSPEQM
CCEEEEEECCCEEEEEEECCCCEEEEEEECEEEEEECCCCCEEHHHHHHCCCCCCCHHHH
NSGAAIGLGVGLYILAIVVGTLVMATILGFKTYDIKKGKLVIAN
CCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]