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The map label for this gene is truA [H]

Identifier: 50364970

GI number: 50364970

Start: 165513

End: 166268

Strand: Direct

Name: truA [H]

Synonym: Mfl155

Alternate gene names: 50364970

Gene position: 165513-166268 (Clockwise)

Preceding gene: 50364969

Following gene: 50364971

Centisome position: 20.87

GC content: 25.26

Gene sequence:

>756_bases
ATGTTTAAATTTTTAATAACTTTAGAATATGATGGTTCAGATTTTCATGGATGAATAGAACAGCCAAAAACTTCTACAAT
TCAAGGAGAACTTAATAAAGCTATTAATAGGGTAACTAAAAATGCAGTTTTTAAAACAATAGGAGCTAGTAAAACTGATA
CAGGAGTACATGCGATTGACCAAAAAGTTTTATTAGATTTAGGTTTTAATCCAAAACTTGATTTATTTAAAAAAGCAATA
AATAAGGCTTTACCTGAAACTATTAAAGTTCGTTCAATAGAAGAAGTTAAACAAGACTTCAATATAAGAGATGTTTTGTA
TAAAGAATATTCTTATACTATAAATGATAAGGAATATAATATACTATCAAATAGGTTTGAACTTAATTGAGATTTTGAAG
AAATAGATATTGATAAGCTACAAAATATATTTAATTTATTTATTGGTGAACATGAATTTAAATTATTTTCTGGATTAAAT
CATAAAGAGTTAGACAGTAACAAAATTACAACAATTAGAGAAATTGAATCTATTGATGTAAAAAGAGCACTTGATAAAGT
TGTTATAACTTTTAAAGCCAAGGGATTTATTAGATATCAAATAAGAATGATTGTGCAGTCAGCCCTTAATTGTTATTTAA
ATAAAAAAATAAGTGCAGATGAAATAAAAGAAAAACTACAAGGGAAGGGTAACAAACCGCCTTTCAATGCTCCTGCAAAG
GGACTTAAATTAAACAAAATAGTTTTTAAATCATAA

Upstream 100 bases:

>100_bases
GTTATTTTTAATTCTGTATTCAGTTAATGTTTTAGGATTATGACACATTAGTTTCCCAAGACTTGACACAATTATTGGAA
TTAGAAATTAAAATATAATA

Downstream 100 bases:

>100_bases
AATAGTGTATTATTATGTTAAGGGTGTTGTTAAAACAATATCACATAAGTAATGCACTGATAAAATAATCGTATGTTTTT
GAATAAGCTTAGGCAAAGAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MFKFLITLEYDGSDFHGWIEQPKTSTIQGELNKAINRVTKNAVFKTIGASKTDTGVHAIDQKVLLDLGFNPKLDLFKKAI
NKALPETIKVRSIEEVKQDFNIRDVLYKEYSYTINDKEYNILSNRFELNWDFEEIDIDKLQNIFNLFIGEHEFKLFSGLN
HKELDSNKITTIREIESIDVKRALDKVVITFKAKGFIRYQIRMIVQSALNCYLNKKISADEIKEKLQGKGNKPPFNAPAK
GLKLNKIVFKS

Sequences:

>Translated_251_residues
MFKFLITLEYDGSDFHG*IEQPKTSTIQGELNKAINRVTKNAVFKTIGASKTDTGVHAIDQKVLLDLGFNPKLDLFKKAI
NKALPETIKVRSIEEVKQDFNIRDVLYKEYSYTINDKEYNILSNRFELN*DFEEIDIDKLQNIFNLFIGEHEFKLFSGLN
HKELDSNKITTIREIESIDVKRALDKVVITFKAKGFIRYQIRMIVQSALNCYLNKKISADEIKEKLQGKGNKPPFNAPAK
GLKLNKIVFKS
>Mature_251_residues
MFKFLITLEYDGSDFHG*IEQPKTSTIQGELNKAINRVTKNAVFKTIGASKTDTGVHAIDQKVLLDLGFNPKLDLFKKAI
NKALPETIKVRSIEEVKQDFNIRDVLYKEYSYTINDKEYNILSNRFELN*DFEEIDIDKLQNIFNLFIGEHEFKLFSGLN
HKELDSNKITTIREIESIDVKRALDKVVITFKAKGFIRYQIRMIVQSALNCYLNKKISADEIKEKLQGKGNKPPFNAPAK
GLKLNKIVFKS

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=210, Percent_Identity=27.1428571428571, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28604; Mature: 28604

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFLITLEYDGSDFHGIEQPKTSTIQGELNKAINRVTKNAVFKTIGASKTDTGVHAIDQ
CEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KVLLDLGFNPKLDLFKKAINKALPETIKVRSIEEVKQDFNIRDVLYKEYSYTINDKEYNI
HHHEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCEEECCCCCHH
LSNRFELNDFEEIDIDKLQNIFNLFIGEHEFKLFSGLNHKELDSNKITTIREIESIDVKR
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
ALDKVVITFKAKGFIRYQIRMIVQSALNCYLNKKISADEIKEKLQGKGNKPPFNAPAKGL
HHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
KLNKIVFKS
EECEEEECC
>Mature Secondary Structure
MFKFLITLEYDGSDFHGIEQPKTSTIQGELNKAINRVTKNAVFKTIGASKTDTGVHAIDQ
CEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
KVLLDLGFNPKLDLFKKAINKALPETIKVRSIEEVKQDFNIRDVLYKEYSYTINDKEYNI
HHHEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHCEEECCCCCHH
LSNRFELNDFEEIDIDKLQNIFNLFIGEHEFKLFSGLNHKELDSNKITTIREIESIDVKR
HHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
ALDKVVITFKAKGFIRYQIRMIVQSALNCYLNKKISADEIKEKLQGKGNKPPFNAPAKGL
HHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
KLNKIVFKS
EECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA