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The map label for this gene is dnaB [H]

Identifier: 50085296

GI number: 50085296

Start: 2159134

End: 2160462

Strand: Reverse

Name: dnaB [H]

Synonym: ACIAD2185

Alternate gene names: 50085296

Gene position: 2160462-2159134 (Counterclockwise)

Preceding gene: 50085297

Following gene: 50085295

Centisome position: 60.04

GC content: 47.25

Gene sequence:

>1329_bases
ATGGTGATGCCAGAATCTCATATTCACAACATCCCAATGGAACAATGCGTTCTGGTGGCACTGATGACTTTTGCACTGTC
CTACGAGCTGATTGCACAAGATCTGGACGAAGGATGTTTTTACCCTGAACGACACAAGCAGATTTACAACGCAATTGTGG
AACTGGCCAATGAAAACCAGCCTTACGATGTCGTCATGGTCGAGCAACGTCTGAAATCTAAAAATCTATTGCACGTATTG
GGCGGTGAGCAATATCTCAAAATCATGTTGGCTGAAGCACCATCAAGTTTTCATAGCATGGAGCCTTACATCACCCAGCT
TAAAAAACTCAAAGCACACAGACAAGTTGAGTGGCTTGGACAACGCATTTCGAGCATGGCACAAGACACGACTCAACCTG
ATGTGTTTGCTGAAGCTGAAAATATACTGGGTCAGGTGGACAACCAAGAGCAAAGCGATCAGGGCATGAGTTTTGAAGCG
GCACTGGACATGGCACTCACCGAAATGATCGAGAAGGCAGAGGCAAAGGACAAGCAGCAATTCACGGGTGTGAAGTTTAA
TCTAAAGCATCTGGATGAATTACTGGGTACCGTGCAAAAGGGGCATTTCTGTGTGGTCGGTGGACGACCAGGAAGTGGTA
AATCCACTTTGGCACAGATGCTGGCATTGGATACCGCCAGTTCGTTTGGTAAGGGCGTATTGTTTATTTCAGCCGAGATG
GACCAGTCTACACTCGCCAACCGTATGATCAGCGCATTAGGGCTAATTCCTTACCACAACATCCACAATGCCGAACTCTA
CAACGGCTTGATGAAAGATTACGCCGATGCCAAAGCGGCCTATCAGCGACTGCCGATCTGGATACAACCGAAACAGAAAC
CCTCCTTGAGTGAGGTCCGTTCCTATGCACGTCGAGCCAAACGCCGTTACAAGGAATTGGGCTGCATCATCGTGGATTAT
TTACAACTGCTCAGAGACCCGAGCAAGAAAGATCGCTTTCAGGAAGTCAGTTCCATCAGCCGTGAATTAAAATCCATGGC
GAAAGAGTTTGAATGTCCTGTCATTGCACTGGTACAGCTCAACCGTGAGGCAGAGAAGGGCAAACGTCCTAAAGCATCAG
ATATCAAAGAATCTGGACAAATCGAGCAAGATGCAGACCAGATCATCCTTGTTAACCCCCTAACCGATCCAGAAGACTCA
ACCAAACCGATGGGAATCACAGAGCTGATTGTAGTGAAAAACCGACACGGTAAACGCGGTGCAGTTCGAGTACTGGATTA
TCTCGAAACTTGTCGTTTTAGATCGGTAGAGGAGCAGGCAGAACCATAA

Upstream 100 bases:

>100_bases
ATCGGTTTATTGGGTATTCACAACAGATTCGTTTTATACCAAAGACACCCGAACCTGAACGCATTGATCTTTCAAAATAC
AAGGTCGTGGAGGATCGAGT

Downstream 100 bases:

>100_bases
AACAATAACGCTTATAAAATTTAACTTGAAACAAATTAGGTGATGAGATGAATACAGCAGTGCACATTATGCAGACGGTG
GATTGGAGCAAGTATGATTT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MVMPESHIHNIPMEQCVLVALMTFALSYELIAQDLDEGCFYPERHKQIYNAIVELANENQPYDVVMVEQRLKSKNLLHVL
GGEQYLKIMLAEAPSSFHSMEPYITQLKKLKAHRQVEWLGQRISSMAQDTTQPDVFAEAENILGQVDNQEQSDQGMSFEA
ALDMALTEMIEKAEAKDKQQFTGVKFNLKHLDELLGTVQKGHFCVVGGRPGSGKSTLAQMLALDTASSFGKGVLFISAEM
DQSTLANRMISALGLIPYHNIHNAELYNGLMKDYADAKAAYQRLPIWIQPKQKPSLSEVRSYARRAKRRYKELGCIIVDY
LQLLRDPSKKDRFQEVSSISRELKSMAKEFECPVIALVQLNREAEKGKRPKASDIKESGQIEQDADQIILVNPLTDPEDS
TKPMGITELIVVKNRHGKRGAVRVLDYLETCRFRSVEEQAEP

Sequences:

>Translated_442_residues
MVMPESHIHNIPMEQCVLVALMTFALSYELIAQDLDEGCFYPERHKQIYNAIVELANENQPYDVVMVEQRLKSKNLLHVL
GGEQYLKIMLAEAPSSFHSMEPYITQLKKLKAHRQVEWLGQRISSMAQDTTQPDVFAEAENILGQVDNQEQSDQGMSFEA
ALDMALTEMIEKAEAKDKQQFTGVKFNLKHLDELLGTVQKGHFCVVGGRPGSGKSTLAQMLALDTASSFGKGVLFISAEM
DQSTLANRMISALGLIPYHNIHNAELYNGLMKDYADAKAAYQRLPIWIQPKQKPSLSEVRSYARRAKRRYKELGCIIVDY
LQLLRDPSKKDRFQEVSSISRELKSMAKEFECPVIALVQLNREAEKGKRPKASDIKESGQIEQDADQIILVNPLTDPEDS
TKPMGITELIVVKNRHGKRGAVRVLDYLETCRFRSVEEQAEP
>Mature_442_residues
MVMPESHIHNIPMEQCVLVALMTFALSYELIAQDLDEGCFYPERHKQIYNAIVELANENQPYDVVMVEQRLKSKNLLHVL
GGEQYLKIMLAEAPSSFHSMEPYITQLKKLKAHRQVEWLGQRISSMAQDTTQPDVFAEAENILGQVDNQEQSDQGMSFEA
ALDMALTEMIEKAEAKDKQQFTGVKFNLKHLDELLGTVQKGHFCVVGGRPGSGKSTLAQMLALDTASSFGKGVLFISAEM
DQSTLANRMISALGLIPYHNIHNAELYNGLMKDYADAKAAYQRLPIWIQPKQKPSLSEVRSYARRAKRRYKELGCIIVDY
LQLLRDPSKKDRFQEVSSISRELKSMAKEFECPVIALVQLNREAEKGKRPKASDIKESGQIEQDADQIILVNPLTDPEDS
TKPMGITELIVVKNRHGKRGAVRVLDYLETCRFRSVEEQAEP

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=436, Percent_Identity=29.8165137614679, Blast_Score=171, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 49961; Mature: 49961

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMPESHIHNIPMEQCVLVALMTFALSYELIAQDLDEGCFYPERHKQIYNAIVELANENQ
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PYDVVMVEQRLKSKNLLHVLGGEQYLKIMLAEAPSSFHSMEPYITQLKKLKAHRQVEWLG
CEEEEHHHHHHCCCCEEEHHCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHH
QRISSMAQDTTQPDVFAEAENILGQVDNQEQSDQGMSFEAALDMALTEMIEKAEAKDKQQ
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTGVKFNLKHLDELLGTVQKGHFCVVGGRPGSGKSTLAQMLALDTASSFGKGVLFISAEM
HCCCEEEHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECC
DQSTLANRMISALGLIPYHNIHNAELYNGLMKDYADAKAAYQRLPIWIQPKQKPSLSEVR
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHH
SYARRAKRRYKELGCIIVDYLQLLRDPSKKDRFQEVSSISRELKSMAKEFECPVIALVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
NREAEKGKRPKASDIKESGQIEQDADQIILVNPLTDPEDSTKPMGITELIVVKNRHGKRG
CCHHHHCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCH
AVRVLDYLETCRFRSVEEQAEP
HHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MVMPESHIHNIPMEQCVLVALMTFALSYELIAQDLDEGCFYPERHKQIYNAIVELANENQ
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
PYDVVMVEQRLKSKNLLHVLGGEQYLKIMLAEAPSSFHSMEPYITQLKKLKAHRQVEWLG
CEEEEHHHHHHCCCCEEEHHCCHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHH
QRISSMAQDTTQPDVFAEAENILGQVDNQEQSDQGMSFEAALDMALTEMIEKAEAKDKQQ
HHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FTGVKFNLKHLDELLGTVQKGHFCVVGGRPGSGKSTLAQMLALDTASSFGKGVLFISAEM
HCCCEEEHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECC
DQSTLANRMISALGLIPYHNIHNAELYNGLMKDYADAKAAYQRLPIWIQPKQKPSLSEVR
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHH
SYARRAKRRYKELGCIIVDYLQLLRDPSKKDRFQEVSSISRELKSMAKEFECPVIALVQL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
NREAEKGKRPKASDIKESGQIEQDADQIILVNPLTDPEDSTKPMGITELIVVKNRHGKRG
CCHHHHCCCCCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCH
AVRVLDYLETCRFRSVEEQAEP
HHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA