Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 50085138

GI number: 50085138

Start: 1993619

End: 1995256

Strand: Reverse

Name: pyrG

Synonym: ACIAD2003

Alternate gene names: 50085138

Gene position: 1995256-1993619 (Counterclockwise)

Preceding gene: 50085141

Following gene: 50085137

Centisome position: 55.45

GC content: 42.25

Gene sequence:

>1638_bases
ATGACCCATTTTATTTTCGTAACTGGTGGTGTTGTTTCATCACTAGGTAAAGGTATTTCAGCTGCTTCAGTTGCTGCACT
TTTAGAAGCCCGTGGTTTAAAAGTAACCATGGTAAAAATGGATCCATATATTAATGTTGATCCGGGGACAATGAGTCCAT
TCCAACACGGTGAAGTTTTTGTTACTGAAGATGGTGCCGAAACAGATTTAGATTTGGGCTATTACGAACGTTTCTTACGT
CGTGCGAAAATGACCAAATTGAATAACTTTACCAGTGGTCGTGTTTATCAAGACGTTTTAAATAAAGAACGTCGTGGCGA
TTACTTAGGCGGTACGGTTCAAGTTATTCCACATATTACCGATAACATCAAAGAGCGTGTTCTTGCAGCTGGTGAAGGCT
ACGATGTTGCGATTGTCGAAATCGGTGGTACGGTTGGTGATATCGAATCTTTACCATTTATGGAATCTGTACGTCAGTTA
ATGGTCGAACTTGGCCATAAGCGTACGATGTTGATGCATTTGACACTCCTCCCATATATTCGTTCAGCAGCAGAGTTGAA
AACCAAACCAACTCAACATTCTGTGAAAGAATTATTGTCAATTGGTATTCAACCAGATATTTTGATCTGCCGTACAGAGC
ATGATGTTGACGCCGACACCAAACGTAAAATTGCATTGTTTACCAATGTTGAAGCACGTGCGGTTGTAGTGTGTAAAGAT
GCGAAAACCATCTATCAAATTCCGCGTAACTTCTACGAGCAAAAAGTAGACGACCTCATCTGTGAGCGTTTCGGCTACAA
CGATTTGCCACAAGCTGATCTTTCAGATTGGGATCAGGTATGCGAAGCGTTATTTAACCCTGAATACATTGTTCGTGTCG
CAATGGTGGGTAAATACGTTGAACTTCCAGATGCGTATAAGTCTGTCAATGAAGCTTTGCTTCATGCGGGTATTCAAAAT
CGCGTAAAAGTTCAGATCGACTATGTCGATGCAGAAACACTTGAAACCCAAGATATCTCTATTTTGTCTACGGCTGATGC
CATCCTTGTACCGGGTGGTTTTGGTGAGCGTGGAACAGAAGGCAAGATGTTGGCCATCAAGTATGCGCGTGAGCAAGGTA
TTCCATTTTTAGGAATCTGCTTGGGCATGCAGCTTGCGGTAATTGAATATGCGCGTAATGTTGCAGGTTTGGCAGAAGCA
ACATCGACTGAATTTAATCGTTCGACCAAGTTCCCAATCATTGGTCTGATCACAGAGTGGTTAGACGAGCGTGGTGAACT
TCAACAGCGCAGCGTTGAGTCAGATCTTGGTGGAACCATGCGTTTAGGTGCACAAAAATCTGAACTGGTCGAAGGCACAA
AAACACGTCAAGTTTATGGTAAAGCTGAGATTGTAGAGCGTCATCGTCACCGCTATGAAATGAACGACCGCTTTATTGAG
CCAATTGAAAAAGCGGGTATGAAAATTTCGGGCTATTCAACTGCGCAACATTTGGTTGAAACAGTCGAAATTCCTGAACA
TCCTTGGTTTATTGCTGTACAATTCCATCCTGAATTTACAAGTTCGCCACGTGATGGACATCCATTATTTGCTAGCTTTA
TCGATGCTGCGAAAAAACAGCACCTTAAAACGAAATAA

Upstream 100 bases:

>100_bases
CTAAGATTTGATGCTGAATGACTTTGCTTTATAGGTGCAGAGTAAAGTAAAATTGCCCAGCATTGCATGCCGATTTAGGC
TCGATTGTACGAGAAACCCA

Downstream 100 bases:

>100_bases
TACGACGCATAAACTAGGACTGTTTAAATGTCACAATTAAAACCCCAAGAGATTGTACGTTTAGGCGATATACAAATGGC
AAATCATTTGCCATTTGTAT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTHFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLR
RAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHITDNIKERVLAAGEGYDVAIVEIGGTVGDIESLPFMESVRQL
MVELGHKRTMLMHLTLLPYIRSAAELKTKPTQHSVKELLSIGIQPDILICRTEHDVDADTKRKIALFTNVEARAVVVCKD
AKTIYQIPRNFYEQKVDDLICERFGYNDLPQADLSDWDQVCEALFNPEYIVRVAMVGKYVELPDAYKSVNEALLHAGIQN
RVKVQIDYVDAETLETQDISILSTADAILVPGGFGERGTEGKMLAIKYAREQGIPFLGICLGMQLAVIEYARNVAGLAEA
TSTEFNRSTKFPIIGLITEWLDERGELQQRSVESDLGGTMRLGAQKSELVEGTKTRQVYGKAEIVERHRHRYEMNDRFIE
PIEKAGMKISGYSTAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKKQHLKTK

Sequences:

>Translated_545_residues
MTHFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLR
RAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHITDNIKERVLAAGEGYDVAIVEIGGTVGDIESLPFMESVRQL
MVELGHKRTMLMHLTLLPYIRSAAELKTKPTQHSVKELLSIGIQPDILICRTEHDVDADTKRKIALFTNVEARAVVVCKD
AKTIYQIPRNFYEQKVDDLICERFGYNDLPQADLSDWDQVCEALFNPEYIVRVAMVGKYVELPDAYKSVNEALLHAGIQN
RVKVQIDYVDAETLETQDISILSTADAILVPGGFGERGTEGKMLAIKYAREQGIPFLGICLGMQLAVIEYARNVAGLAEA
TSTEFNRSTKFPIIGLITEWLDERGELQQRSVESDLGGTMRLGAQKSELVEGTKTRQVYGKAEIVERHRHRYEMNDRFIE
PIEKAGMKISGYSTAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKKQHLKTK
>Mature_544_residues
THFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVFVTEDGAETDLDLGYYERFLRR
AKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHITDNIKERVLAAGEGYDVAIVEIGGTVGDIESLPFMESVRQLM
VELGHKRTMLMHLTLLPYIRSAAELKTKPTQHSVKELLSIGIQPDILICRTEHDVDADTKRKIALFTNVEARAVVVCKDA
KTIYQIPRNFYEQKVDDLICERFGYNDLPQADLSDWDQVCEALFNPEYIVRVAMVGKYVELPDAYKSVNEALLHAGIQNR
VKVQIDYVDAETLETQDISILSTADAILVPGGFGERGTEGKMLAIKYAREQGIPFLGICLGMQLAVIEYARNVAGLAEAT
STEFNRSTKFPIIGLITEWLDERGELQQRSVESDLGGTMRLGAQKSELVEGTKTRQVYGKAEIVERHRHRYEMNDRFIEP
IEKAGMKISGYSTAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKKQHLKTK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=560, Percent_Identity=43.0357142857143, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI28559085, Length=560, Percent_Identity=41.7857142857143, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI28559083, Length=560, Percent_Identity=41.7857142857143, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI221316689, Length=560, Percent_Identity=41.7857142857143, Blast_Score=442, Evalue=1e-124,
Organism=Escherichia coli, GI1789142, Length=538, Percent_Identity=63.5687732342007, Blast_Score=709, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=613, Percent_Identity=36.5415986949429, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322563, Length=572, Percent_Identity=40.2097902097902, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319432, Length=569, Percent_Identity=40.9490333919156, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=40.9335727109515, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=38.9662027833002, Blast_Score=350, Evalue=2e-96,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_ACIAD (Q6FAT7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046648.1
- HSSP:   P17812
- ProteinModelPortal:   Q6FAT7
- SMR:   Q6FAT7
- STRING:   Q6FAT7
- GeneID:   2879568
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2003
- NMPDR:   fig|62977.3.peg.1910
- eggNOG:   COG0504
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- PhylomeDB:   Q6FAT7
- ProtClustDB:   PRK05380
- BioCyc:   ASP62977:ACIAD2003-MONOMER
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 60963; Mature: 60831

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 380-380 ACT_SITE 516-516 ACT_SITE 518-518

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVF
CCEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGYYERFLRRAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHIT
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHH
DNIKERVLAAGEGYDVAIVEIGGTVGDIESLPFMESVRQLMVELGHKRTMLMHLTLLPYI
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RSAAELKTKPTQHSVKELLSIGIQPDILICRTEHDVDADTKRKIALFTNVEARAVVVCKD
HHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEEECC
AKTIYQIPRNFYEQKVDDLICERFGYNDLPQADLSDWDQVCEALFNPEYIVRVAMVGKYV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEHHHHHCCHH
ELPDAYKSVNEALLHAGIQNRVKVQIDYVDAETLETQDISILSTADAILVPGGFGERGTE
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCC
GKMLAIKYAREQGIPFLGICLGMQLAVIEYARNVAGLAEATSTEFNRSTKFPIIGLITEW
CCEEEEEEHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LDERGELQQRSVESDLGGTMRLGAQKSELVEGTKTRQVYGKAEIVERHRHRYEMNDRFIE
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
PIEKAGMKISGYSTAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKKQ
HHHHCCCEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HLKTK
HCCCC
>Mature Secondary Structure 
THFIFVTGGVVSSLGKGISAASVAALLEARGLKVTMVKMDPYINVDPGTMSPFQHGEVF
CEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEEE
VTEDGAETDLDLGYYERFLRRAKMTKLNNFTSGRVYQDVLNKERRGDYLGGTVQVIPHIT
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHH
DNIKERVLAAGEGYDVAIVEIGGTVGDIESLPFMESVRQLMVELGHKRTMLMHLTLLPYI
HHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RSAAELKTKPTQHSVKELLSIGIQPDILICRTEHDVDADTKRKIALFTNVEARAVVVCKD
HHHHHHCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEEEEECC
AKTIYQIPRNFYEQKVDDLICERFGYNDLPQADLSDWDQVCEALFNPEYIVRVAMVGKYV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHEEHHHHHCCHH
ELPDAYKSVNEALLHAGIQNRVKVQIDYVDAETLETQDISILSTADAILVPGGFGERGTE
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCC
GKMLAIKYAREQGIPFLGICLGMQLAVIEYARNVAGLAEATSTEFNRSTKFPIIGLITEW
CCEEEEEEHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LDERGELQQRSVESDLGGTMRLGAQKSELVEGTKTRQVYGKAEIVERHRHRYEMNDRFIE
HHHHHHHHHHHHHHHCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHH
PIEKAGMKISGYSTAQHLVETVEIPEHPWFIAVQFHPEFTSSPRDGHPLFASFIDAAKKQ
HHHHCCCEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HLKTK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA