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The map label for this gene is murB

Identifier: 50085084

GI number: 50085084

Start: 1934862

End: 1935899

Strand: Reverse

Name: murB

Synonym: ACIAD1945

Alternate gene names: 50085084

Gene position: 1935899-1934862 (Counterclockwise)

Preceding gene: 50085085

Following gene: 50085083

Centisome position: 53.8

GC content: 38.54

Gene sequence:

>1038_bases
GTGATACACATTCAACATCAGGTTCAGCTCAAACCGTTCAATACACTTAATTTGGATGTGGTCGCTTCACATTATATTAA
AGTTCAGTCTGTGAATGAGCTCATTGAAGCGCTTGATTATGCAGAGCAGCAACAGCTCAATGCCTTGATTTTATCTGGTG
GTAGTAACATGCTGCTGCCACAAAGCCTGGATGCTTTGGTCTTACATATAGATATTCAGGGTCTGGAAATGATCGACGAG
GATTCTGAATCTGTAAAAATTAAAGTCGGTGGTGGTCAGATATGGCATGATTTTGTTTTAATGACCACGCAAAAGGGCTG
GTATGGTTTACAAAATCTTGCGTTAATTCCAGGATTGGTTGGAGCGTCACCTGTACAAAATATTGGAGCTTACGGCGTTG
AAGTTGGTGAGTTTATTGAGTTGGTCCATGTCTATGATCGTAGTGAAAAAAGTGTAAAAACGATTTCATGTGCCGATTGT
AAGTTTGCATATCGCCATAGTATTTTTAAAGACGAACCTTATCGTTATATAATTACTCATGTCACTTTTAAATTGTTTAA
AACGCCAAATTTAAAATTAAGTTATGGCGATTTACTCAAAGCTGTGGGAAATGAGCAAACACCTGAAAATTTGCAAAAAC
AGGTCATACAGATTCGTGAAAGCAAACTTCCAAATCCTAAAGAGTATCCAAATGTGGGCAGTTTTTTTAAAAATCCTGTG
ATTTCTCGACAAGATTTTAACCAGTTGCTTACAGAATTCCCGCTCATTCCACATTATCCTCAACCCCATGATCGTGTAAA
AATTGCGGCAGGTTGGTTGATTGATCAGGCGGGTTGGAAGGGAAAGCGACTTGGTGCTGTTGGAATGTTTGAAAAACAAG
CTTTAGTTCTGGTGAATTATGCCAATGCAACCTTGATAGATGTGCAGCACACTTATCGTCATGTTCAGTCTGATATACAA
CAGAAATTTAAACTTCGACTTGAGCCTGAACCCGTTCTTTTTGGTTCTAATGGGTTAATTCTTTCGCATATGGAATAA

Upstream 100 bases:

>100_bases
TGAACGCGTACTCAATCAATGCGAATCAAGTGCTCAAGCGTGGGTTGCGATCTTTAAACGTGATCAGTCTGAATAAATCG
TTCAAATACAGGTTTCTATT

Downstream 100 bases:

>100_bases
CGATATATGTCACAAACACATTGGATGATTCGCTTGGTCCGAATAGTAACGATACTATTTGTTCTGATCGCTTGTCTGAT
GATTTTTATTTATACGCCTT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MIHIQHQVQLKPFNTLNLDVVASHYIKVQSVNELIEALDYAEQQQLNALILSGGSNMLLPQSLDALVLHIDIQGLEMIDE
DSESVKIKVGGGQIWHDFVLMTTQKGWYGLQNLALIPGLVGASPVQNIGAYGVEVGEFIELVHVYDRSEKSVKTISCADC
KFAYRHSIFKDEPYRYIITHVTFKLFKTPNLKLSYGDLLKAVGNEQTPENLQKQVIQIRESKLPNPKEYPNVGSFFKNPV
ISRQDFNQLLTEFPLIPHYPQPHDRVKIAAGWLIDQAGWKGKRLGAVGMFEKQALVLVNYANATLIDVQHTYRHVQSDIQ
QKFKLRLEPEPVLFGSNGLILSHME

Sequences:

>Translated_345_residues
MIHIQHQVQLKPFNTLNLDVVASHYIKVQSVNELIEALDYAEQQQLNALILSGGSNMLLPQSLDALVLHIDIQGLEMIDE
DSESVKIKVGGGQIWHDFVLMTTQKGWYGLQNLALIPGLVGASPVQNIGAYGVEVGEFIELVHVYDRSEKSVKTISCADC
KFAYRHSIFKDEPYRYIITHVTFKLFKTPNLKLSYGDLLKAVGNEQTPENLQKQVIQIRESKLPNPKEYPNVGSFFKNPV
ISRQDFNQLLTEFPLIPHYPQPHDRVKIAAGWLIDQAGWKGKRLGAVGMFEKQALVLVNYANATLIDVQHTYRHVQSDIQ
QKFKLRLEPEPVLFGSNGLILSHME
>Mature_345_residues
MIHIQHQVQLKPFNTLNLDVVASHYIKVQSVNELIEALDYAEQQQLNALILSGGSNMLLPQSLDALVLHIDIQGLEMIDE
DSESVKIKVGGGQIWHDFVLMTTQKGWYGLQNLALIPGLVGASPVQNIGAYGVEVGEFIELVHVYDRSEKSVKTISCADC
KFAYRHSIFKDEPYRYIITHVTFKLFKTPNLKLSYGDLLKAVGNEQTPENLQKQVIQIRESKLPNPKEYPNVGSFFKNPV
ISRQDFNQLLTEFPLIPHYPQPHDRVKIAAGWLIDQAGWKGKRLGAVGMFEKQALVLVNYANATLIDVQHTYRHVQSDIQ
QKFKLRLEPEPVLFGSNGLILSHME

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=332, Percent_Identity=40.9638554216867, Blast_Score=245, Evalue=4e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_ACIAD (Q6FAZ1)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046594.1
- ProteinModelPortal:   Q6FAZ1
- SMR:   Q6FAZ1
- STRING:   Q6FAZ1
- GeneID:   2879154
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1945
- NMPDR:   fig|62977.3.peg.1856
- eggNOG:   COG0812
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- PhylomeDB:   Q6FAZ1
- ProtClustDB:   PRK00046
- BioCyc:   ASP62977:ACIAD1945-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 39024; Mature: 39024

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 165-165 ACT_SITE 234-234 ACT_SITE 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHIQHQVQLKPFNTLNLDVVASHYIKVQSVNELIEALDYAEQQQLNALILSGGSNMLLP
CEEEEEEEEECCCCCCCCEEHHHHHEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECC
QSLDALVLHIDIQGLEMIDEDSESVKIKVGGGQIWHDFVLMTTQKGWYGLQNLALIPGLV
CCCCEEEEEEEECCCEEECCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCHHHHHCCCC
GASPVQNIGAYGVEVGEFIELVHVYDRSEKSVKTISCADCKFAYRHSIFKDEPYRYIITH
CCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCEEEEEE
VTFKLFKTPNLKLSYGDLLKAVGNEQTPENLQKQVIQIRESKLPNPKEYPNVGSFFKNPV
EEEEEECCCCCEECHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCC
ISRQDFNQLLTEFPLIPHYPQPHDRVKIAAGWLIDQAGWKGKRLGAVGMFEKQALVLVNY
CCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEE
ANATLIDVQHTYRHVQSDIQQKFKLRLEPEPVLFGSNGLILSHME
CCEEEEEHHHHHHHHHHHHHHHHHEECCCCCEEECCCCEEEEECC
>Mature Secondary Structure
MIHIQHQVQLKPFNTLNLDVVASHYIKVQSVNELIEALDYAEQQQLNALILSGGSNMLLP
CEEEEEEEEECCCCCCCCEEHHHHHEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECC
QSLDALVLHIDIQGLEMIDEDSESVKIKVGGGQIWHDFVLMTTQKGWYGLQNLALIPGLV
CCCCEEEEEEEECCCEEECCCCCEEEEEECCCEEEEEEEEEEECCCCCCCCHHHHHCCCC
GASPVQNIGAYGVEVGEFIELVHVYDRSEKSVKTISCADCKFAYRHSIFKDEPYRYIITH
CCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCEEEEEE
VTFKLFKTPNLKLSYGDLLKAVGNEQTPENLQKQVIQIRESKLPNPKEYPNVGSFFKNPV
EEEEEECCCCCEECHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCC
ISRQDFNQLLTEFPLIPHYPQPHDRVKIAAGWLIDQAGWKGKRLGAVGMFEKQALVLVNY
CCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEE
ANATLIDVQHTYRHVQSDIQQKFKLRLEPEPVLFGSNGLILSHME
CCEEEEEHHHHHHHHHHHHHHHHHEECCCCCEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA