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The map label for this gene is braE [H]

Identifier: 50085078

GI number: 50085078

Start: 1928463

End: 1929533

Strand: Reverse

Name: braE [H]

Synonym: ACIAD1939

Alternate gene names: 50085078

Gene position: 1929533-1928463 (Counterclockwise)

Preceding gene: 50085079

Following gene: 50085077

Centisome position: 53.62

GC content: 39.31

Gene sequence:

>1071_bases
ATGTTTTTATTTTCTCAAGCTGAACAGTTTATTCGTCGTTATCAGGATGAAAAAAGAATTTTTGCGCTCAAACAACATCG
CATATTGTTCTGGTTTTTTGTTGCTATTGCTTTTTTATTTATCCCTTTTGTTGGGTCTGATTATTTATTTAATGCGATTC
TGGTTCCTTACCTTGTACTGGCACTGGCTGGATTGGGTCTCAATATTTTAACTGGTTTTACAGGTCAGTTATCTCTGGGA
TCAGCAGCATTTATGGCTGTTGGTGCATTTGCAACATATAACTTTGAGCTCCGTATTCCTCAATTACCTTTACTCCTCAG
TTTGTTTCTTGGGGGAACGATAGCGGCAGTTTTTGGTGTGATTGTGGGATTGCCAAGCCTGCGGATTAAAGGGTTTTACC
TGATTGTCTCAACTCTGGCAGCACAGTTTTTTATTCCTTGGTTATTTACACAATATGGTTGGTTTACCAACTATAGCGCG
TCAGGAGTGATTACCGCGCCCAAGTTGGTGATTCTTGGATATTCATTAGATTCTCCAGTTGGACATTATCTTTTAACAAT
CAGCATCGTTCTTGGGTTGACTTTGATTGCACGTAATCTCCTGAATAGTCAGTATGGTCGTAATTTCCAAGCGATTCGTG
ATATGGAAACAGCCGCGGTGACCTTAGGTATTTCTGCTGCCAAGACCAAGTTGCTCGCATTTGCAATCAGTTCATTTTAT
TTAGGTGTTGCGGGTGCTCTTTGGGCCTTTGCTTATCTGGGAACAATTGAAGCCAATGGGCTTGATCTCGATCGCTCATT
TCAGATTCTGTTCATCATCATTATTGGTGGATTGAGTAGTCTGGCAGGAAGCTTTTTTGGAGCCGCCTTTATTGTGCTGC
TTCCGATCATTTTAAGTTTTTTGAGTAACTTGTTTTTTGGTCAAGGTTTTGATCAGGCATTACTACAAAATATCCAGAAA
ATTATTTTTGGTATTTTAATTATTTATTTTCTTATTCGCGAACCTGAAGGGCTTGTACGTCTTTATCAGCGTTTTTATAC
ACGTGTTAGAAAGTGGCCTTTGCGCTATTAA

Upstream 100 bases:

>100_bases
GTGGCATAGAAAACTGGTTTCCATACCTCATCGCAATTTTATTTTTACTGATACGTCCGACAGGGCTATTTGGTGAACAA
CAAGTTGCGAGGGTATAACG

Downstream 100 bases:

>100_bases
TTATTCACAAAAAATAGGGGTAAACATGAAATTTTTTAAGTCATTGTGGTTTTGGTTGGTGCTGATTGGACTCGTACTGG
CTGCTATCGTGTATTGGACA

Product: branched chain amino acid ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MFLFSQAEQFIRRYQDEKRIFALKQHRILFWFFVAIAFLFIPFVGSDYLFNAILVPYLVLALAGLGLNILTGFTGQLSLG
SAAFMAVGAFATYNFELRIPQLPLLLSLFLGGTIAAVFGVIVGLPSLRIKGFYLIVSTLAAQFFIPWLFTQYGWFTNYSA
SGVITAPKLVILGYSLDSPVGHYLLTISIVLGLTLIARNLLNSQYGRNFQAIRDMETAAVTLGISAAKTKLLAFAISSFY
LGVAGALWAFAYLGTIEANGLDLDRSFQILFIIIIGGLSSLAGSFFGAAFIVLLPIILSFLSNLFFGQGFDQALLQNIQK
IIFGILIIYFLIREPEGLVRLYQRFYTRVRKWPLRY

Sequences:

>Translated_356_residues
MFLFSQAEQFIRRYQDEKRIFALKQHRILFWFFVAIAFLFIPFVGSDYLFNAILVPYLVLALAGLGLNILTGFTGQLSLG
SAAFMAVGAFATYNFELRIPQLPLLLSLFLGGTIAAVFGVIVGLPSLRIKGFYLIVSTLAAQFFIPWLFTQYGWFTNYSA
SGVITAPKLVILGYSLDSPVGHYLLTISIVLGLTLIARNLLNSQYGRNFQAIRDMETAAVTLGISAAKTKLLAFAISSFY
LGVAGALWAFAYLGTIEANGLDLDRSFQILFIIIIGGLSSLAGSFFGAAFIVLLPIILSFLSNLFFGQGFDQALLQNIQK
IIFGILIIYFLIREPEGLVRLYQRFYTRVRKWPLRY
>Mature_356_residues
MFLFSQAEQFIRRYQDEKRIFALKQHRILFWFFVAIAFLFIPFVGSDYLFNAILVPYLVLALAGLGLNILTGFTGQLSLG
SAAFMAVGAFATYNFELRIPQLPLLLSLFLGGTIAAVFGVIVGLPSLRIKGFYLIVSTLAAQFFIPWLFTQYGWFTNYSA
SGVITAPKLVILGYSLDSPVGHYLLTISIVLGLTLIARNLLNSQYGRNFQAIRDMETAAVTLGISAAKTKLLAFAISSFY
LGVAGALWAFAYLGTIEANGLDLDRSFQILFIIIIGGLSSLAGSFFGAAFIVLLPIILSFLSNLFFGQGFDQALLQNIQK
IIFGILIIYFLIREPEGLVRLYQRFYTRVRKWPLRY

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=307, Percent_Identity=27.6872964169381, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39651; Mature: 39651

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLFSQAEQFIRRYQDEKRIFALKQHRILFWFFVAIAFLFIPFVGSDYLFNAILVPYLVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALAGLGLNILTGFTGQLSLGSAAFMAVGAFATYNFELRIPQLPLLLSLFLGGTIAAVFGV
HHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHH
IVGLPSLRIKGFYLIVSTLAAQFFIPWLFTQYGWFTNYSASGVITAPKLVILGYSLDSPV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCEEEEEECCCCCCH
GHYLLTISIVLGLTLIARNLLNSQYGRNFQAIRDMETAAVTLGISAAKTKLLAFAISSFY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LGVAGALWAFAYLGTIEANGLDLDRSFQILFIIIIGGLSSLAGSFFGAAFIVLLPIILSF
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSNLFFGQGFDQALLQNIQKIIFGILIIYFLIREPEGLVRLYQRFYTRVRKWPLRY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFLFSQAEQFIRRYQDEKRIFALKQHRILFWFFVAIAFLFIPFVGSDYLFNAILVPYLVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALAGLGLNILTGFTGQLSLGSAAFMAVGAFATYNFELRIPQLPLLLSLFLGGTIAAVFGV
HHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHH
IVGLPSLRIKGFYLIVSTLAAQFFIPWLFTQYGWFTNYSASGVITAPKLVILGYSLDSPV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCEEEEEECCCCCCH
GHYLLTISIVLGLTLIARNLLNSQYGRNFQAIRDMETAAVTLGISAAKTKLLAFAISSFY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LGVAGALWAFAYLGTIEANGLDLDRSFQILFIIIIGGLSSLAGSFFGAAFIVLLPIILSF
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSNLFFGQGFDQALLQNIQKIIFGILIIYFLIREPEGLVRLYQRFYTRVRKWPLRY
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]