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The map label for this gene is flbD [H]

Identifier: 50085074

GI number: 50085074

Start: 1923845

End: 1924966

Strand: Reverse

Name: flbD [H]

Synonym: ACIAD1935

Alternate gene names: 50085074

Gene position: 1924966-1923845 (Counterclockwise)

Preceding gene: 50085075

Following gene: 50085071

Centisome position: 53.49

GC content: 39.57

Gene sequence:

>1122_bases
TTGGTTATTATTATGAGTCGGTATTCGGCACCTCAAATTTTTTCTCTTGAAGGAGAGCAGGCCCAACCTTTAAGTATTCG
CGCGAAAGCGTTGGTTTATCACGATCCTGTATCGAAAAATTTACTTCAACTGATTGAACGCTTGGCACCCAATGATATTC
CTATTCTGATTCAGGGTGAAACGGGAACGGGAAAAGAGCTAGTTGCACGACATATCCACCATTTGAGTGGACGCAAAGGT
CCATTCCTGGCTGTTAATTGTGGTGCTATTAATGATCAATTGGCCGAGAGTGAGTTATTTGGACACGAAGCGGGTTCCTT
TACTGGAGCAACTGGACAACGTAAAGGTTGGTTTGAATCTGCTGATGGCGGAACCTTATTTTTAGATGAGATTGGTGACC
TACCTTTACATCTACAGGTAAAGCTATTGCGCGTATTGCAAGAGCGTGAAGTGACACGGGTTGGGGCAAGAAAAGCCATA
CCTGTGGATATACGTCTGGTCACAGCCACCAACGTCGATTTAGAGCAGGCCGTTCAAGCAGAACAATTCAGACAAGATTT
ACTGTTTAGAATTAATATTGCTCAATTAGAGCTGAAGCCTTTAAGAGAGCGCAAAGGTGATATTCTGCCACTCTTTCAGC
ATTTTCTACAAAAATATGGCGAAAAAATTAAGAAAGAAGCCAACTTAATCCTTTCTCCTACAGTGGTTACAGCATTATAT
GATTATCCTTGGCCAGGAAATATTCGTGAACTTGAGAATATTGCACATTACGCTGTACTGGTTGCAGAAGGGCATTTAAT
TAAGAAAGAGCATCTTAAGTTTAACAAACTATTTCAAAATTTTAATATTGAACAACCAACCTCTGAAACTGCAAAGAGCT
GGGAGAATCCGTTTGATGGGATTGAATATCAACTCAAGCGAATTTTTGCTGCACCCACGCTTCCTTCTAATTTATGGGAT
AGGCTAGAACAGCTAATCATTGCGACATCTTTTGAGTATTGTCAGAGAAATCAGGTTCAAACCTCTGTGTTATTAGGAAT
TACACGAAATGTAATGCGAACTTATTTAAAGCGACATAATTTAGTCAATGATGCTTTAAACTTTAAAATTGTGAATGAAT
AA

Upstream 100 bases:

>100_bases
ATTTTTTTTATAATAAATTAATCTCATATTCTCGGTGAAAATTGATATAAACCCTTTCACCGAGTTCGCTTAATATCGAC
TCAATCGTATTTTATCCCAT

Downstream 100 bases:

>100_bases
AAACAATAAGGCGTATTCAAGTCGAATCATTGGAATACGCCCCATATGGCTTATTTTTGCTTAAAAACGATCAGCCATCG
CCATGTTCCACATTCGGTAG

Product: sigma-54 dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MVIIMSRYSAPQIFSLEGEQAQPLSIRAKALVYHDPVSKNLLQLIERLAPNDIPILIQGETGTGKELVARHIHHLSGRKG
PFLAVNCGAINDQLAESELFGHEAGSFTGATGQRKGWFESADGGTLFLDEIGDLPLHLQVKLLRVLQEREVTRVGARKAI
PVDIRLVTATNVDLEQAVQAEQFRQDLLFRINIAQLELKPLRERKGDILPLFQHFLQKYGEKIKKEANLILSPTVVTALY
DYPWPGNIRELENIAHYAVLVAEGHLIKKEHLKFNKLFQNFNIEQPTSETAKSWENPFDGIEYQLKRIFAAPTLPSNLWD
RLEQLIIATSFEYCQRNQVQTSVLLGITRNVMRTYLKRHNLVNDALNFKIVNE

Sequences:

>Translated_373_residues
MVIIMSRYSAPQIFSLEGEQAQPLSIRAKALVYHDPVSKNLLQLIERLAPNDIPILIQGETGTGKELVARHIHHLSGRKG
PFLAVNCGAINDQLAESELFGHEAGSFTGATGQRKGWFESADGGTLFLDEIGDLPLHLQVKLLRVLQEREVTRVGARKAI
PVDIRLVTATNVDLEQAVQAEQFRQDLLFRINIAQLELKPLRERKGDILPLFQHFLQKYGEKIKKEANLILSPTVVTALY
DYPWPGNIRELENIAHYAVLVAEGHLIKKEHLKFNKLFQNFNIEQPTSETAKSWENPFDGIEYQLKRIFAAPTLPSNLWD
RLEQLIIATSFEYCQRNQVQTSVLLGITRNVMRTYLKRHNLVNDALNFKIVNE
>Mature_373_residues
MVIIMSRYSAPQIFSLEGEQAQPLSIRAKALVYHDPVSKNLLQLIERLAPNDIPILIQGETGTGKELVARHIHHLSGRKG
PFLAVNCGAINDQLAESELFGHEAGSFTGATGQRKGWFESADGGTLFLDEIGDLPLHLQVKLLRVLQEREVTRVGARKAI
PVDIRLVTATNVDLEQAVQAEQFRQDLLFRINIAQLELKPLRERKGDILPLFQHFLQKYGEKIKKEANLILSPTVVTALY
DYPWPGNIRELENIAHYAVLVAEGHLIKKEHLKFNKLFQNFNIEQPTSETAKSWENPFDGIEYQLKRIFAAPTLPSNLWD
RLEQLIIATSFEYCQRNQVQTSVLLGITRNVMRTYLKRHNLVNDALNFKIVNE

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=237, Percent_Identity=51.8987341772152, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI1788550, Length=346, Percent_Identity=39.8843930635838, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI1790299, Length=344, Percent_Identity=40.406976744186, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790437, Length=244, Percent_Identity=49.5901639344262, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1789233, Length=287, Percent_Identity=43.5540069686411, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789087, Length=230, Percent_Identity=49.1304347826087, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI87081872, Length=325, Percent_Identity=38.7692307692308, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786524, Length=237, Percent_Identity=48.1012658227848, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1788905, Length=241, Percent_Identity=43.9834024896266, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI87082152, Length=268, Percent_Identity=42.5373134328358, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=271, Percent_Identity=36.1623616236162, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1789828, Length=298, Percent_Identity=31.8791946308725, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI87081858, Length=337, Percent_Identity=28.1899109792285, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 42370; Mature: 42370

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIIMSRYSAPQIFSLEGEQAQPLSIRAKALVYHDPVSKNLLQLIERLAPNDIPILIQGE
CEEEEECCCCCCEEECCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECC
TGTGKELVARHIHHLSGRKGPFLAVNCGAINDQLAESELFGHEAGSFTGATGQRKGWFES
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ADGGTLFLDEIGDLPLHLQVKLLRVLQEREVTRVGARKAIPVDIRLVTATNVDLEQAVQA
CCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHH
EQFRQDLLFRINIAQLELKPLRERKGDILPLFQHFLQKYGEKIKKEANLILSPTVVTALY
HHHHHHHHEEEEHHHEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHC
DYPWPGNIRELENIAHYAVLVAEGHLIKKEHLKFNKLFQNFNIEQPTSETAKSWENPFDG
CCCCCCCHHHHHHHHHHEEEEECCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCH
IEYQLKRIFAAPTLPSNLWDRLEQLIIATSFEYCQRNQVQTSVLLGITRNVMRTYLKRHN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
LVNDALNFKIVNE
HHHHCCCEEEECC
>Mature Secondary Structure
MVIIMSRYSAPQIFSLEGEQAQPLSIRAKALVYHDPVSKNLLQLIERLAPNDIPILIQGE
CEEEEECCCCCCEEECCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEECC
TGTGKELVARHIHHLSGRKGPFLAVNCGAINDQLAESELFGHEAGSFTGATGQRKGWFES
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ADGGTLFLDEIGDLPLHLQVKLLRVLQEREVTRVGARKAIPVDIRLVTATNVDLEQAVQA
CCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHH
EQFRQDLLFRINIAQLELKPLRERKGDILPLFQHFLQKYGEKIKKEANLILSPTVVTALY
HHHHHHHHEEEEHHHEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHC
DYPWPGNIRELENIAHYAVLVAEGHLIKKEHLKFNKLFQNFNIEQPTSETAKSWENPFDG
CCCCCCCHHHHHHHHHHEEEEECCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCH
IEYQLKRIFAAPTLPSNLWDRLEQLIIATSFEYCQRNQVQTSVLLGITRNVMRTYLKRHN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
LVNDALNFKIVNE
HHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]