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The map label for this gene is gidB

Identifier: 49188319

GI number: 49188319

Start: 5222235

End: 5222954

Strand: Reverse

Name: gidB

Synonym: BAS5335

Alternate gene names: 49188319

Gene position: 5222954-5222235 (Counterclockwise)

Preceding gene: 49188320

Following gene: 49188318

Centisome position: 99.89

GC content: 36.81

Gene sequence:

>720_bases
ATGAACATAGAACAATTTCAATCTATGCTAGAAGAGAAGGGTATCACCCTCTCTTCTAGACAGTTAGAGCAGTTCGAAAT
CTACTTCGAAACATTAGTAGAATGGAATGAAAAAATGAATTTAACGGCTATTACGGAGAAAGAGGAAGTATATTTAAAAC
ACTTTTTTGATTCCATTACAGCTGCTTTTTATTATGATTTTTCAAAACCATTCTCAATTTGTGATGTTGGCGCAGGAGCT
GGATTCCCAAGTATTCCTTTAAAAATTTGTTTCCCGCATTTAAAAGTGACAATTGTAGACTCATTGCAAAAACGTATTAA
TTTCTTAAATCATTTAGCACAAAAGTTGGAATTAAGTGACGTCGCGTTTTGTCACGATCGTGCTGAAACATTTGGGAAAA
AAGAAGGTGTACGTGAAGCGTACGACATTGTAATGGCACGTGCAGTCGCGCGTCTTTCAGTATTAAGTGAGCTATGTTTA
CCGCTTGTAAAAGTTGGGGGAACATTCATTGCAATGAAAGGTGCCGCAGCAAACGAAGAAATTGAAAATGGCAAGTATGC
TTTAGAGGTGCTAGGCGGGGATCTAAAGGAAATGTCTACGTTCCAATTACCGTTTGAAGAAAGTGAACGTAATATTTTAT
TAATCGAGAAAAAGCGCAAGACACCAAAGAAATATCCACGCAAACCGGGAACACCCAATAAATTACCTATTGAAAAATAA

Upstream 100 bases:

>100_bases
TGGCGTAAATCCAGCGGACATTTCCATTTTACTTGTGTATATTGAACAAGGAAAAATTGCGCGAGTATCGAACCAATAAA
TAGTAAGGAGATATTGTTAG

Downstream 100 bases:

>100_bases
ATCTTATTAGACGTAAGATTAAATTATATAAATGAAAACGTAAATGTTTCAAAGGAAACATTTTATGGAGAATAGTCAAT
AGGCCTAAAGGTGGTGGAAT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSKPFSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAANEEIENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK

Sequences:

>Translated_239_residues
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSKPFSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAANEEIENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK
>Mature_239_residues
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSITAAFYYDFSKPFSICDVGAGA
GFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSDVAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCL
PLVKVGGTFIAMKGAAANEEIENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK

Specific function: Specifically methylates the N7 position of guanosine in position 535 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=217, Percent_Identity=30.4147465437788, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_BACAA (C3P3F3)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869691.1
- ProteinModelPortal:   C3P3F3
- SMR:   C3P3F3
- EnsemblBacteria:   EBBACT00000129145
- GeneID:   7852753
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5766
- GeneTree:   EBGT00050000000933
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 27196; Mature: 27196

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSIT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
AAFYYDFSKPFSICDVGAGAGFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSD
HHHHHCCCCCCCCEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCLPLVKVGGTFIAMKGAAANEE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
IENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK
HHCCCEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNIEQFQSMLEEKGITLSSRQLEQFEIYFETLVEWNEKMNLTAITEKEEVYLKHFFDSIT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHH
AAFYYDFSKPFSICDVGAGAGFPSIPLKICFPHLKVTIVDSLQKRINFLNHLAQKLELSD
HHHHHCCCCCCCCEECCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFCHDRAETFGKKEGVREAYDIVMARAVARLSVLSELCLPLVKVGGTFIAMKGAAANEE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
IENGKYALEVLGGDLKEMSTFQLPFEESERNILLIEKKRKTPKKYPRKPGTPNKLPIEK
HHCCCEEEEECCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA