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The map label for this gene is ssb [H]

Identifier: 49188310

GI number: 49188310

Start: 5215240

End: 5215761

Strand: Reverse

Name: ssb [H]

Synonym: BAS5326

Alternate gene names: 49188310

Gene position: 5215761-5215240 (Counterclockwise)

Preceding gene: 49188311

Following gene: 49188309

Centisome position: 99.75

GC content: 42.72

Gene sequence:

>522_bases
TTGATGAATCGTGTTATCCTCGTTGGTCGTTTAACTAAGGACCCTGACTTACGTTACACGCCCAATGGTGTTGCAGTAGC
TACTTTTACGTTAGCTGTGAATCGCGCATTTGCGAATCAACAAGGTGAGCGTGAAGCTGACTTTATTAATTGTGTAATAT
GGCGTAAACAAGCAGAAAACGTAGCAAATTATTTAAAAAAAGGTAGCTTAGCAGGCGTAGATGGACGTCTTCAAACTCGT
AATTACGAGGGACAAGATGGTAAGCGTGTATATGTAACAGAAGTTCTTGCGGAAAGCGTACAATTTTTAGAGCCGCGTAA
TGGCGGTGGGGAGCAACGTGGTTCATTTAATCAGCAACCATCAGGAGCTGGTTTCGGTAACCAAAGCTCTAACCCATTTG
GTCAATCTAGTAATTCGGGCAACCAAGGTAACCAAGGAAACTCTGGATTTACGAAGAATGACGATCCATTTTCAAATGTA
GGTCAACCGATCGACATTTCCGACGACGATTTACCGTTCTAA

Upstream 100 bases:

>100_bases
ACGAATTCGATCGTTTAGCTAAGATCAACGAAGATATCCTTCGTCATATCGTTGTTAAAGAAGAAGAAAAATAATTGATA
TATAAGGGAGAGTGGTTCGA

Downstream 100 bases:

>100_bases
TACAGTAAATTTGTTAGTTTTAACAAAGAATGGAGGGAAATAGACATGGCAGGACGCAAAGGTGGACGTGCGAAACGTCG
TAAGGTGTGTTTCTTCACAT

Product: single-stranded DNA-binding protein

Products: NA

Alternate protein names: SSB; Helix-destabilizing protein [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MMNRVILVGRLTKDPDLRYTPNGVAVATFTLAVNRAFANQQGEREADFINCVIWRKQAENVANYLKKGSLAGVDGRLQTR
NYEGQDGKRVYVTEVLAESVQFLEPRNGGGEQRGSFNQQPSGAGFGNQSSNPFGQSSNSGNQGNQGNSGFTKNDDPFSNV
GQPIDISDDDLPF

Sequences:

>Translated_173_residues
MMNRVILVGRLTKDPDLRYTPNGVAVATFTLAVNRAFANQQGEREADFINCVIWRKQAENVANYLKKGSLAGVDGRLQTR
NYEGQDGKRVYVTEVLAESVQFLEPRNGGGEQRGSFNQQPSGAGFGNQSSNPFGQSSNSGNQGNQGNSGFTKNDDPFSNV
GQPIDISDDDLPF
>Mature_173_residues
MMNRVILVGRLTKDPDLRYTPNGVAVATFTLAVNRAFANQQGEREADFINCVIWRKQAENVANYLKKGSLAGVDGRLQTR
NYEGQDGKRVYVTEVLAESVQFLEPRNGGGEQRGSFNQQPSGAGFGNQSSNPFGQSSNSGNQGNQGNSGFTKNDDPFSNV
GQPIDISDDDLPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair [H]

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain [H]

Homologues:

Organism=Escherichia coli, GI1790494, Length=115, Percent_Identity=40, Blast_Score=83, Evalue=8e-18,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344 [H]

Pfam domain/function: PF00436 SSB [H]

EC number: NA

Molecular weight: Translated: 18824; Mature: 18824

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNRVILVGRLTKDPDLRYTPNGVAVATFTLAVNRAFANQQGEREADFINCVIWRKQAEN
CCCCEEEEEECCCCCCCEECCCCEEEEEHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHH
VANYLKKGSLAGVDGRLQTRNYEGQDGKRVYVTEVLAESVQFLEPRNGGGEQRGSFNQQP
HHHHHHCCCCCCCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SGAGFGNQSSNPFGQSSNSGNQGNQGNSGFTKNDDPFSNVGQPIDISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MMNRVILVGRLTKDPDLRYTPNGVAVATFTLAVNRAFANQQGEREADFINCVIWRKQAEN
CCCCEEEEEECCCCCCCEECCCCEEEEEHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHH
VANYLKKGSLAGVDGRLQTRNYEGQDGKRVYVTEVLAESVQFLEPRNGGGEQRGSFNQQP
HHHHHHCCCCCCCCCEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SGAGFGNQSSNPFGQSSNSGNQGNQGNSGFTKNDDPFSNVGQPIDISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]