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The map label for this gene is rfbX [C]

Identifier: 49188264

GI number: 49188264

Start: 5163196

End: 5164650

Strand: Direct

Name: rfbX [C]

Synonym: BAS5279

Alternate gene names: 49188264

Gene position: 5163196-5164650 (Clockwise)

Preceding gene: 49188263

Following gene: 49188265

Centisome position: 98.75

GC content: 30.1

Gene sequence:

>1455_bases
ATGCAGAAATCAATTGCTTCAAATATTTTTTATAAAATACTGCTCAATACGTTTAATATTATCCTTCCAATTTTAGTTGG
TCCTTACGCATATCGAACGTTAGGAGCAACTTCAATCGGAACCGTTAATCTTGCTGAAACTTTTTTCAATTACTTCTTCG
TGTTTGCCGTATTTGGCGTATATCAATATGGTTTACGCGAAATTAGCTTAATTAAAAACGATAAGAAGAAAGTTTCTAAG
TTATTTACAAGTCTCTATGTAATTAACTTCACAACTAGTATTTTAGCTTTATCATCCTTTGTGCTCTTTAGCTATATTGG
ATATGGACATGAAAGTCTATTTCCTGTTCTTCTTATTTTCGGTTTTAATTTTATATCTAACATGTTTTATGTGGAATGGT
TTAATGAAGCATATGAAAATTATGACTTCATCACTAAAAAAACAGTTATCGTTCGATTAATCTATGTCGTACTTCTTTTC
TCTTTCATTCATGGGGTTGACGATTATAAAACATATGCTGGTTTATTAGTATTATCAACATTTTTAAACCACTCTATTAG
CTTTGTATATGTGAAACGCCAAGTGAAATTTGATTTTTCCGACTTAACGATTATTCCTCATTTAAAACCGTTACTATTAG
TATTGATTTTTTCAAATGCTAATATTTTATATACTCAATTAGATCGTCTTGTTTTAGGAGTATATGTTGGAAAAGAAGAA
GTTGCATATTACGTAATGGCTTTTCAAATTATGATGATTATTAACACACTTATGTTAAGTGTTGTACAAGTAACCGTTCC
AAGGCTATCTTACTTATCAGGAAATGCATCTGAAATGGAGTATGAATCTTTACTAAATAAAATTTCAAAAGTATATTTCA
TTACCCTATTCCCTGCTGCAATTGGTTTAATGCTCATTGCTCATGGAGCTGTTGTTATTTACGGTGGACAACAATTTGCT
GCAGCTGGTAATACATTAATTGTCTTCGCCTTCTATATGATTACGGTTGGTATTGAGTCCATTTTATCGAATCAAATTAT
TTATGTGAAAAAGAAAGAAAGCATTTTAGTGCGATTTTTATTCATTTGCGGATTTATCAACTTAGCATCTAATATTGCAC
TCATATTCTTCCATGTACTTACACCAACAACAGCGATTTTCACAACAACAATTGCGAACTGCTTCTTAATTACATTTGAA
TATATTTATGTGAAAAAGAAACTAAAAGTGAATTATACGTTATTCGATATACAAAAACTTAAATATCTATTTTATTCCCT
AACATTTATACCGATAGCATTCGTTATTGATATGATTGTATCTGGACAAATACTACAAGTTATCCTAGTAATAATAACGT
GTGGATTAACGTATGCCCTTATCCTCTTTATAACAAAGGATGAAATTCTCTTTTCGCTACTTGAAAAGATACTAGCACGA
TTCAAAAAGGCTTAA

Upstream 100 bases:

>100_bases
CACTTCATTATATTGACAGAATTAACTGTAGTTAAAAACCTTACTATGACATAGCAAGTATTTTAATTAAATTGGATTAT
TTTAATGGAAAGGTGAAATT

Downstream 100 bases:

>100_bases
ACGCAATCTCTAAAAGGAGCTGTTAAACTTGAAATTTTCTTTAATCATGGCTACTTGTGGCAGACGTGATGATATTCTTG
ACTTACTAAAATCACTTGAA

Product: polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: Flippase Wzx; PST Family Polysaccharide Transporter; Repeat Unit Transporter; Polysaccharide Transporter; LPS Biosynthesis Related Flippase; Polysaccharide Transporter PST Family; O-Antigen Transporter; PST System Family Polysaccharide Transporter; Oligosaccharide Translocase; Heteropolysaccharide Repeat-Containing Protein; Polysaccharide Transporter Protein; Polymerization And Export Protein; Polysaccharide Transporter Pst Family; Wzx Flippase; RfbX Protein; CPS Repeat-Containing Transporter; Polysaccharide Synthase Family Protein; Oligosaccharide Repeat-Containing Transporter; Polysaccharide Repeat-Containing Transporter; Oligosaccharide Repeat-Containing Polymerase; Oligosaccharide Repeat Unit Transporter; Spore Cortex Biosynthesis Protein; PTS System Polysaccharide Transporter

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MQKSIASNIFYKILLNTFNIILPILVGPYAYRTLGATSIGTVNLAETFFNYFFVFAVFGVYQYGLREISLIKNDKKKVSK
LFTSLYVINFTTSILALSSFVLFSYIGYGHESLFPVLLIFGFNFISNMFYVEWFNEAYENYDFITKKTVIVRLIYVVLLF
SFIHGVDDYKTYAGLLVLSTFLNHSISFVYVKRQVKFDFSDLTIIPHLKPLLLVLIFSNANILYTQLDRLVLGVYVGKEE
VAYYVMAFQIMMIINTLMLSVVQVTVPRLSYLSGNASEMEYESLLNKISKVYFITLFPAAIGLMLIAHGAVVIYGGQQFA
AAGNTLIVFAFYMITVGIESILSNQIIYVKKKESILVRFLFICGFINLASNIALIFFHVLTPTTAIFTTTIANCFLITFE
YIYVKKKLKVNYTLFDIQKLKYLFYSLTFIPIAFVIDMIVSGQILQVILVIITCGLTYALILFITKDEILFSLLEKILAR
FKKA

Sequences:

>Translated_484_residues
MQKSIASNIFYKILLNTFNIILPILVGPYAYRTLGATSIGTVNLAETFFNYFFVFAVFGVYQYGLREISLIKNDKKKVSK
LFTSLYVINFTTSILALSSFVLFSYIGYGHESLFPVLLIFGFNFISNMFYVEWFNEAYENYDFITKKTVIVRLIYVVLLF
SFIHGVDDYKTYAGLLVLSTFLNHSISFVYVKRQVKFDFSDLTIIPHLKPLLLVLIFSNANILYTQLDRLVLGVYVGKEE
VAYYVMAFQIMMIINTLMLSVVQVTVPRLSYLSGNASEMEYESLLNKISKVYFITLFPAAIGLMLIAHGAVVIYGGQQFA
AAGNTLIVFAFYMITVGIESILSNQIIYVKKKESILVRFLFICGFINLASNIALIFFHVLTPTTAIFTTTIANCFLITFE
YIYVKKKLKVNYTLFDIQKLKYLFYSLTFIPIAFVIDMIVSGQILQVILVIITCGLTYALILFITKDEILFSLLEKILAR
FKKA
>Mature_484_residues
MQKSIASNIFYKILLNTFNIILPILVGPYAYRTLGATSIGTVNLAETFFNYFFVFAVFGVYQYGLREISLIKNDKKKVSK
LFTSLYVINFTTSILALSSFVLFSYIGYGHESLFPVLLIFGFNFISNMFYVEWFNEAYENYDFITKKTVIVRLIYVVLLF
SFIHGVDDYKTYAGLLVLSTFLNHSISFVYVKRQVKFDFSDLTIIPHLKPLLLVLIFSNANILYTQLDRLVLGVYVGKEE
VAYYVMAFQIMMIINTLMLSVVQVTVPRLSYLSGNASEMEYESLLNKISKVYFITLFPAAIGLMLIAHGAVVIYGGQQFA
AAGNTLIVFAFYMITVGIESILSNQIIYVKKKESILVRFLFICGFINLASNIALIFFHVLTPTTAIFTTTIANCFLITFE
YIYVKKKLKVNYTLFDIQKLKYLFYSLTFIPIAFVIDMIVSGQILQVILVIITCGLTYALILFITKDEILFSLLEKILAR
FKKA

Specific function: May Be Involved In The Translocation Process Of The Nascent O-Polysaccharide Molecules And/Or Its Ligation To Lipid A Core Units. [C]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 55268; Mature: 55268

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSIASNIFYKILLNTFNIILPILVGPYAYRTLGATSIGTVNLAETFFNYFFVFAVFGV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
YQYGLREISLIKNDKKKVSKLFTSLYVINFTTSILALSSFVLFSYIGYGHESLFPVLLIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GFNFISNMFYVEWFNEAYENYDFITKKTVIVRLIYVVLLFSFIHGVDDYKTYAGLLVLST
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FLNHSISFVYVKRQVKFDFSDLTIIPHLKPLLLVLIFSNANILYTQLDRLVLGVYVGKEE
HHCCCEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEECHHH
VAYYVMAFQIMMIINTLMLSVVQVTVPRLSYLSGNASEMEYESLLNKISKVYFITLFPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGLMLIAHGAVVIYGGQQFAAAGNTLIVFAFYMITVGIESILSNQIIYVKKKESILVRFL
HHHHHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
FICGFINLASNIALIFFHVLTPTTAIFTTTIANCFLITFEYIYVKKKLKVNYTLFDIQKL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEHEEEEEEEHHHHHH
KYLFYSLTFIPIAFVIDMIVSGQILQVILVIITCGLTYALILFITKDEILFSLLEKILAR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKA
HCCC
>Mature Secondary Structure
MQKSIASNIFYKILLNTFNIILPILVGPYAYRTLGATSIGTVNLAETFFNYFFVFAVFGV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
YQYGLREISLIKNDKKKVSKLFTSLYVINFTTSILALSSFVLFSYIGYGHESLFPVLLIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GFNFISNMFYVEWFNEAYENYDFITKKTVIVRLIYVVLLFSFIHGVDDYKTYAGLLVLST
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FLNHSISFVYVKRQVKFDFSDLTIIPHLKPLLLVLIFSNANILYTQLDRLVLGVYVGKEE
HHCCCEEEEEEEEEEEECCCCEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEECHHH
VAYYVMAFQIMMIINTLMLSVVQVTVPRLSYLSGNASEMEYESLLNKISKVYFITLFPAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGLMLIAHGAVVIYGGQQFAAAGNTLIVFAFYMITVGIESILSNQIIYVKKKESILVRFL
HHHHHHHCCCEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHH
FICGFINLASNIALIFFHVLTPTTAIFTTTIANCFLITFEYIYVKKKLKVNYTLFDIQKL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEHEEEEEEEHHHHHH
KYLFYSLTFIPIAFVIDMIVSGQILQVILVIITCGLTYALILFITKDEILFSLLEKILAR
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKKA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA