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The map label for this gene is spo0F [H]

Identifier: 49188171

GI number: 49188171

Start: 5065837

End: 5066205

Strand: Reverse

Name: spo0F [H]

Synonym: BAS5185

Alternate gene names: 49188171

Gene position: 5066205-5065837 (Counterclockwise)

Preceding gene: 49188173

Following gene: 49188170

Centisome position: 96.89

GC content: 33.6

Gene sequence:

>369_bases
ATGGAAGGGAAAATTTTAATCGTTGATGATCAATATGGCATTCGTGTGTTATTACATGAAGTGTTCCAAAAAGAAGGTTA
TCAGACATTCCAAGCAGCGAATGGATTTCAAGCTTTAGATATCGTGAAAAAAGATAATCCAGATTTAGTTGTGTTAGATA
TGAAAATTCCAGGTATGGATGGTATAGAGATTTTAAAGCATGTAAAAGAAATTGATGAGAGTATTAAAGTGATTTTAATG
ACTGCTTATGGTGAGCTTGATATGATCCAAGAAGCAAAAGATTTAGGTGCTTTAATGCATTTTGCTAAACCATTTGATAT
TGATGAAATTCGTCAAGCGGTGAGAAATGAGCTCGCTGTAGAGGCTTAA

Upstream 100 bases:

>100_bases
TAAGAAAGAATAGGAAGGAATTTGGCAAGAAATAGCGAAAATAAATCGTTAAGATATAAGAAGCTTTAAATCTTGTATTT
ACACAGAAAGGTTGGGAGTT

Downstream 100 bases:

>100_bases
AAATGAATTTTTTATAAAAAATATGGAAAAAAGCGTTTACATGGACTATTGTGCAGGTGAATACGGTTGAGTTTTTGAGT
ACAAGTTGTTATCCTATTGA

Product: stage 0 sporulation protein F

Products: NA

Alternate protein names: Stage 0 sporulation protein F [H]

Number of amino acids: Translated: 122; Mature: 122

Protein sequence:

>122_residues
MEGKILIVDDQYGIRVLLHEVFQKEGYQTFQAANGFQALDIVKKDNPDLVVLDMKIPGMDGIEILKHVKEIDESIKVILM
TAYGELDMIQEAKDLGALMHFAKPFDIDEIRQAVRNELAVEA

Sequences:

>Translated_122_residues
MEGKILIVDDQYGIRVLLHEVFQKEGYQTFQAANGFQALDIVKKDNPDLVVLDMKIPGMDGIEILKHVKEIDESIKVILM
TAYGELDMIQEAKDLGALMHFAKPFDIDEIRQAVRNELAVEA
>Mature_122_residues
MEGKILIVDDQYGIRVLLHEVFQKEGYQTFQAANGFQALDIVKKDNPDLVVLDMKIPGMDGIEILKHVKEIDESIKVILM
TAYGELDMIQEAKDLGALMHFAKPFDIDEIRQAVRNELAVEA

Specific function: Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation [H]

COG id: COG0784

COG function: function code T; FOG: CheY-like receiver

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=120, Percent_Identity=39.1666666666667, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1790299, Length=107, Percent_Identity=36.4485981308411, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1790437, Length=115, Percent_Identity=36.5217391304348, Blast_Score=77, Evalue=3e-16,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=32.5, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1786599, Length=112, Percent_Identity=32.1428571428571, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1787487, Length=112, Percent_Identity=32.1428571428571, Blast_Score=63, Evalue=6e-12,
Organism=Escherichia coli, GI1788905, Length=118, Percent_Identity=29.6610169491525, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=31.7073170731707, Blast_Score=61, Evalue=3e-11,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=30.9090909090909, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 13801; Mature: 13801

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGKILIVDDQYGIRVLLHEVFQKEGYQTFQAANGFQALDIVKKDNPDLVVLDMKIPGMD
CCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCC
GIEILKHVKEIDESIKVILMTAYGELDMIQEAKDLGALMHFAKPFDIDEIRQAVRNELAV
HHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
EA
CC
>Mature Secondary Structure
MEGKILIVDDQYGIRVLLHEVFQKEGYQTFQAANGFQALDIVKKDNPDLVVLDMKIPGMD
CCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCC
GIEILKHVKEIDESIKVILMTAYGELDMIQEAKDLGALMHFAKPFDIDEIRQAVRNELAV
HHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
EA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7514590 [H]