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The map label for this gene is ndhF [H]

Identifier: 49188128

GI number: 49188128

Start: 5026915

End: 5028777

Strand: Reverse

Name: ndhF [H]

Synonym: BAS5142

Alternate gene names: 49188128

Gene position: 5028777-5026915 (Counterclockwise)

Preceding gene: 49188129

Following gene: 49188127

Centisome position: 96.18

GC content: 42.19

Gene sequence:

>1863_bases
ATGATCGATTATGTATGGCTCATACCGCTTTTCCCGCTTGTTTCGTTTTTGTTATTAATTATATTCGGGAAGAAAATTAG
AGAAGGAAGCAGTGTACTTAGTATTTTCTTCGTCTTTCTCTCCTTTATATGTGCGGTACTTGTATTAATAGAAAGACTTT
CGACAGTACCAGTGAAGCATAAGTGGGTATGGCTTAGAACTGGAGATATTGATATATCGTTTGGCTTTGAAGTGACAGCA
TTAGGGGCTTTAATGTTGTTTATCGTTACACTTGTGAGTTTACTTGTACATGTGTATTCGAAAGGTTATATGAAAGGTGA
GGAAAGATTACCAACCTTTTATGCCTATTTAGGGCTCTTTACATTTGCGATGCTCGGGCTCGTTATATCACCAAATTTAT
TACAGCTTTATATATTTTGGGAGTTAGTTGGCCTTGGCTCATTTTTACTCATCGGTTTTTATTTCTTTAAAGAAGAAGCG
AGGGCTGCCGCGAAGAAGGCTTTTATTATGACACGTATTGGAGATATCGGTTTATTTATTGGGATGATCTTAATTTTCTG
GCATGCAGGTAGTTTTGAATATGACGCAATATTTAGGGCGATTCATACGGGTGATCTTACCCCTACGATGATTACAATAA
CGGCGATATTAATTTTTATCGGGGCGATGGGAAAATCAGGTCAGTTCCCCCTTCATACGTGGTTACCGGATGCGATGGAA
GGGCCGACGCCTGTTTCGGCACTCATTCATGCGGCGACGATGGTCGCAGCTGGCGTATATTTAGTGGCAACGATGTTTCC
GCTATTTTCAGCAAGTGCGGTGGCGATGCAAACGGTGGCAATCGTTGGAGCTTTCACCGCGATCTTTGCGGCTTCCATCG
GTCTTGTGCAAACGGATATAAAGAGAGTGCTTGCTTATTCTACAGTTAGTCAGCTTGGGTATATGATGCTTGCATTAGGA
TCTGCCGGTTATGTAGCTGGTGTGTTCCATTTAACGACACACGCCTTTTTTAAAGCGTTGCTATTTTTGGCGGCAGGAAG
TGTCATTCATGCCGTGCATACGCAAAACATTAATAAAATGGGCGGCTTACAGAAGAAGATGAAAGTAACTGCTGCGCTAT
TTTTAATCGGTACCCTTGCAATAAGTGGTGTGCCGCTCCTTTCCGGTTTCTTTAGTAAAGATGAAATTTTGGCGGCGACT
TGGATGAACGGCAATTACGTTTTATTCATATTAGCAGTAATTGCAGCATTCCTAACAGCGTTCTACATGTTCCGCTTATA
CTTTCTCGTCTTTACGGGGGAAGCGAAGACGAAGGTAGATGTGCATGAATCGCCTCGCATCATGACATATCCGATGATTG
TCCTTGGCGTCCTTGCAGTCGTGGCAGGTTATATAAATACACCGTGGTTTGGAACGTTTCTCGGAGGTTGGCTTACGAAA
GATGTAGCATTTAAAGTGCAAGAAGCACACGGCCCAGTTTGGATTATGATTGTTGCGACGCTCGTTTCTTTGGCTGGGAT
TGCTCTTGCATATTTCATATATGGCAAGAAATCTATTGCTAGAGATTGGGCTGGTGGCGAGGAAGCACCTCTTTATAAAA
TTTTGCAAGAAAAATATTATGTGGATGAACTATACAATATGACAGTCATTCCTATCACGAAAGGAATCGCTCATGTGCTT
CACTTATTTGAAGTGTATGTAGTCGAAGGGATTGCCGTTTTAATAGGGAGTTTAGTTAAAGGGGTGAGCGGAGTCGGCTC
TAGACTCCAAAACGGAAATGTACAAGTGTACGGGACTGTAACAGCTGTTTCACTTGCAGTGCTCTTCATTATTTTGCTCT
ATACGGGAGCGGATTTACGATGA

Upstream 100 bases:

>100_bases
CCATTTTAATTGCTTTATATCGTAACCGTACGACAGTTCATGTAGATGAAATGGATACGCTCAAAGGATAGCATTGTGAC
CGAAAAGGGGGAAGGAAACA

Downstream 100 bases:

>100_bases
ATGATTTGTTATTAACGTTCTTCATTTTTTCCCCGCTTTTAGGAATTCTCTTACTTGCATTAACACCGAAAAAAGAATCG
CGTACAGTGCGAGCGCTCGG

Product: NADH dehydrogenase subunit L

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 5; NDH-1, chain 5 [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MIDYVWLIPLFPLVSFLLLIIFGKKIREGSSVLSIFFVFLSFICAVLVLIERLSTVPVKHKWVWLRTGDIDISFGFEVTA
LGALMLFIVTLVSLLVHVYSKGYMKGEERLPTFYAYLGLFTFAMLGLVISPNLLQLYIFWELVGLGSFLLIGFYFFKEEA
RAAAKKAFIMTRIGDIGLFIGMILIFWHAGSFEYDAIFRAIHTGDLTPTMITITAILIFIGAMGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVATMFPLFSASAVAMQTVAIVGAFTAIFAASIGLVQTDIKRVLAYSTVSQLGYMMLALG
SAGYVAGVFHLTTHAFFKALLFLAAGSVIHAVHTQNINKMGGLQKKMKVTAALFLIGTLAISGVPLLSGFFSKDEILAAT
WMNGNYVLFILAVIAAFLTAFYMFRLYFLVFTGEAKTKVDVHESPRIMTYPMIVLGVLAVVAGYINTPWFGTFLGGWLTK
DVAFKVQEAHGPVWIMIVATLVSLAGIALAYFIYGKKSIARDWAGGEEAPLYKILQEKYYVDELYNMTVIPITKGIAHVL
HLFEVYVVEGIAVLIGSLVKGVSGVGSRLQNGNVQVYGTVTAVSLAVLFIILLYTGADLR

Sequences:

>Translated_620_residues
MIDYVWLIPLFPLVSFLLLIIFGKKIREGSSVLSIFFVFLSFICAVLVLIERLSTVPVKHKWVWLRTGDIDISFGFEVTA
LGALMLFIVTLVSLLVHVYSKGYMKGEERLPTFYAYLGLFTFAMLGLVISPNLLQLYIFWELVGLGSFLLIGFYFFKEEA
RAAAKKAFIMTRIGDIGLFIGMILIFWHAGSFEYDAIFRAIHTGDLTPTMITITAILIFIGAMGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVATMFPLFSASAVAMQTVAIVGAFTAIFAASIGLVQTDIKRVLAYSTVSQLGYMMLALG
SAGYVAGVFHLTTHAFFKALLFLAAGSVIHAVHTQNINKMGGLQKKMKVTAALFLIGTLAISGVPLLSGFFSKDEILAAT
WMNGNYVLFILAVIAAFLTAFYMFRLYFLVFTGEAKTKVDVHESPRIMTYPMIVLGVLAVVAGYINTPWFGTFLGGWLTK
DVAFKVQEAHGPVWIMIVATLVSLAGIALAYFIYGKKSIARDWAGGEEAPLYKILQEKYYVDELYNMTVIPITKGIAHVL
HLFEVYVVEGIAVLIGSLVKGVSGVGSRLQNGNVQVYGTVTAVSLAVLFIILLYTGADLR
>Mature_620_residues
MIDYVWLIPLFPLVSFLLLIIFGKKIREGSSVLSIFFVFLSFICAVLVLIERLSTVPVKHKWVWLRTGDIDISFGFEVTA
LGALMLFIVTLVSLLVHVYSKGYMKGEERLPTFYAYLGLFTFAMLGLVISPNLLQLYIFWELVGLGSFLLIGFYFFKEEA
RAAAKKAFIMTRIGDIGLFIGMILIFWHAGSFEYDAIFRAIHTGDLTPTMITITAILIFIGAMGKSGQFPLHTWLPDAME
GPTPVSALIHAATMVAAGVYLVATMFPLFSASAVAMQTVAIVGAFTAIFAASIGLVQTDIKRVLAYSTVSQLGYMMLALG
SAGYVAGVFHLTTHAFFKALLFLAAGSVIHAVHTQNINKMGGLQKKMKVTAALFLIGTLAISGVPLLSGFFSKDEILAAT
WMNGNYVLFILAVIAAFLTAFYMFRLYFLVFTGEAKTKVDVHESPRIMTYPMIVLGVLAVVAGYINTPWFGTFLGGWLTK
DVAFKVQEAHGPVWIMIVATLVSLAGIALAYFIYGKKSIARDWAGGEEAPLYKILQEKYYVDELYNMTVIPITKGIAHVL
HLFEVYVVEGIAVLIGSLVKGVSGVGSRLQNGNVQVYGTVTAVSLAVLFIILLYTGADLR

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=409, Percent_Identity=37.4083129584352, Blast_Score=263, Evalue=3e-70,
Organism=Escherichia coli, GI1788614, Length=566, Percent_Identity=40.8127208480565, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1788829, Length=424, Percent_Identity=33.7264150943396, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788831, Length=414, Percent_Identity=31.6425120772947, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788827, Length=420, Percent_Identity=30, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1788613, Length=395, Percent_Identity=26.5822784810127, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI2367154, Length=430, Percent_Identity=24.8837209302326, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693160, Length=354, Percent_Identity=26.271186440678, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR002128
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01010 Oxidored_q1_C; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 67877; Mature: 67877

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYVWLIPLFPLVSFLLLIIFGKKIREGSSVLSIFFVFLSFICAVLVLIERLSTVPVKH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
KWVWLRTGDIDISFGFEVTALGALMLFIVTLVSLLVHVYSKGYMKGEERLPTFYAYLGLF
EEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHH
TFAMLGLVISPNLLQLYIFWELVGLGSFLLIGFYFFKEEARAAAKKAFIMTRIGDIGLFI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMILIFWHAGSFEYDAIFRAIHTGDLTPTMITITAILIFIGAMGKSGQFPLHTWLPDAME
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC
GPTPVSALIHAATMVAAGVYLVATMFPLFSASAVAMQTVAIVGAFTAIFAASIGLVQTDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRVLAYSTVSQLGYMMLALGSAGYVAGVFHLTTHAFFKALLFLAAGSVIHAVHTQNINKM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHH
GGLQKKMKVTAALFLIGTLAISGVPLLSGFFSKDEILAATWMNGNYVLFILAVIAAFLTA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
FYMFRLYFLVFTGEAKTKVDVHESPRIMTYPMIVLGVLAVVAGYINTPWFGTFLGGWLTK
HHHHHHHHHHEECCCCCEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
DVAFKVQEAHGPVWIMIVATLVSLAGIALAYFIYGKKSIARDWAGGEEAPLYKILQEKYY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHH
VDELYNMTVIPITKGIAHVLHLFEVYVVEGIAVLIGSLVKGVSGVGSRLQNGNVQVYGTV
HHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
TAVSLAVLFIILLYTGADLR
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIDYVWLIPLFPLVSFLLLIIFGKKIREGSSVLSIFFVFLSFICAVLVLIERLSTVPVKH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
KWVWLRTGDIDISFGFEVTALGALMLFIVTLVSLLVHVYSKGYMKGEERLPTFYAYLGLF
EEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHHHHHHH
TFAMLGLVISPNLLQLYIFWELVGLGSFLLIGFYFFKEEARAAAKKAFIMTRIGDIGLFI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMILIFWHAGSFEYDAIFRAIHTGDLTPTMITITAILIFIGAMGKSGQFPLHTWLPDAME
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCC
GPTPVSALIHAATMVAAGVYLVATMFPLFSASAVAMQTVAIVGAFTAIFAASIGLVQTDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRVLAYSTVSQLGYMMLALGSAGYVAGVFHLTTHAFFKALLFLAAGSVIHAVHTQNINKM
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHH
GGLQKKMKVTAALFLIGTLAISGVPLLSGFFSKDEILAATWMNGNYVLFILAVIAAFLTA
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
FYMFRLYFLVFTGEAKTKVDVHESPRIMTYPMIVLGVLAVVAGYINTPWFGTFLGGWLTK
HHHHHHHHHHEECCCCCEEECCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
DVAFKVQEAHGPVWIMIVATLVSLAGIALAYFIYGKKSIARDWAGGEEAPLYKILQEKYY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHH
VDELYNMTVIPITKGIAHVLHLFEVYVVEGIAVLIGSLVKGVSGVGSRLQNGNVQVYGTV
HHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
TAVSLAVLFIILLYTGADLR
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8501038; 1554697 [H]