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The map label for this gene is dat [H]

Identifier: 49188068

GI number: 49188068

Start: 4961141

End: 4962025

Strand: Direct

Name: dat [H]

Synonym: BAS5082

Alternate gene names: 49188068

Gene position: 4961141-4962025 (Clockwise)

Preceding gene: 49188067

Following gene: 49188069

Centisome position: 94.88

GC content: 37.51

Gene sequence:

>885_bases
ATGGGACGAAAATTGGCATATGAAAGATTTGTACTTTGGAATGATGCAATTATTGATACAACGAAGCAAAAAACGTACAT
AGAACTTGAAGAAAGAGGCTTGCAGTTTGGAGATGGTGTCTACGAGGTTATTCGTCTATATAAAGGGAACTTCCACTTAT
TAGATCCCCATATCACAAGATTATATCGCTTCATGGAAGAAATAGAATTAACACTCCCTTTCTCAAAAGCAGAACTGATT
ACCCTACTTTATAAACTAATCGAAAATAATAATTTCCACGAAGATGGAACGATTTATTTGCAAGTATCTCGTGGTGTACA
AGCTCGTACCCATACGTTCTCATATGACGTCCCTCCGACAATCTATGCTTATATTACAAAGAAAGAAAGACCAGCGCTAT
GGATTGAATATGGTGTACGTGCTATATCAGAACCGGATACACGCTGGCTACGCTGTGATATCAAATCATTAAATTTATTA
CCAAATATATTAGCTGCTACGAAAGCGGAACGAAAAGGTTGTAAAGAAGCCCTTTTCGTACGAAATGGTACTGTAACTGA
GGGAAGTCACTCTAACTTCTTTCTCATTAAAAACGGAACTCTTTACACACATCCAGCTAATCACCTTATTTTAAATGGCA
TTATTCGTCAATATGTCCTTTCTTTAGCGAAAACCCTTCGTATTCCAGTACAAGAAGAGCTTTTCAGCATTCGTGATGTT
TATCAAGCGGATGAATGTTTCTTTACAGGAACGACGATTGAAATTTTGCCGATGACTCACCTTGATGGAACCGCAATCCA
AGATGGTCAAGTTGGCCCTATCACTAAAATGCTGCAAAGATCATTTTCTCAAAGTTTGTTACAATCCAACATGTCATCCT
CTTAA

Upstream 100 bases:

>100_bases
ATATACGTCATATGTAAAGAGTTACTCACATAAAAATTACTATAGTGATTTTTATTCCGAATGAATTACAGTTTCACTTT
ACTTATGCACTTACGAAAGG

Downstream 100 bases:

>100_bases
ACACGAATAATTTCTATCCTTTCTACATTCAATATATGTATTTAAATGGAAATCAAGGTATAGATTTATGCCTTGATTTT
TTCTTGTAATCTTTTTGTCA

Product: D-amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MGRKLAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRFMEEIELTLPFSKAELI
TLLYKLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLL
PNILAATKAERKGCKEALFVRNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDV
YQADECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS

Sequences:

>Translated_294_residues
MGRKLAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRFMEEIELTLPFSKAELI
TLLYKLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLL
PNILAATKAERKGCKEALFVRNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDV
YQADECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS
>Mature_293_residues
GRKLAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITRLYRFMEEIELTLPFSKAELIT
LLYKLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPTIYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLP
NILAATKAERKGCKEALFVRNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDVY
QADECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=293, Percent_Identity=28.6689419795222, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787338, Length=255, Percent_Identity=26.2745098039216, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 33834; Mature: 33703

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKLAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITR
CCCCCCHHEEEEECCCEEECCCCCEEEEEHHCCCCCCCHHHHHHHHHCCCEEEECHHHHH
LYRFMEEIELTLPFSKAELITLLYKLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPT
HHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCE
IYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLPNILAATKAERKGCKEALFV
EEEEEECCCCCEEEEECCCHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCHHHHEEE
RNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDV
ECCCEECCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YQADECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS
HHCCCEEEECCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GRKLAYERFVLWNDAIIDTTKQKTYIELEERGLQFGDGVYEVIRLYKGNFHLLDPHITR
CCCCCHHEEEEECCCEEECCCCCEEEEEHHCCCCCCCHHHHHHHHHCCCEEEECHHHHH
LYRFMEEIELTLPFSKAELITLLYKLIENNNFHEDGTIYLQVSRGVQARTHTFSYDVPPT
HHHHHHHHHEECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCCCCE
IYAYITKKERPALWIEYGVRAISEPDTRWLRCDIKSLNLLPNILAATKAERKGCKEALFV
EEEEEECCCCCEEEEECCCHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCHHHHEEE
RNGTVTEGSHSNFFLIKNGTLYTHPANHLILNGIIRQYVLSLAKTLRIPVQEELFSIRDV
ECCCEECCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
YQADECFFTGTTIEILPMTHLDGTAIQDGQVGPITKMLQRSFSQSLLQSNMSSS
HHCCCEEEECCEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2644261; 2914916; 7626635; 9538014; 9749913 [H]