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The map label for this gene is speE [H]

Identifier: 49188046

GI number: 49188046

Start: 4935331

End: 4936413

Strand: Direct

Name: speE [H]

Synonym: BAS5060

Alternate gene names: 49188046

Gene position: 4935331-4936413 (Clockwise)

Preceding gene: 49188040

Following gene: 49188047

Centisome position: 94.39

GC content: 35.27

Gene sequence:

>1083_bases
ATGCCAAAACATAGAAAACAAAGCAAAATAAAGATTTATAGAATAACAAACTATAAAAAAGACAAGCGTTCTGAATTAGA
CTCTAACAAATTTGAACTAGAACAGCAGGCGGTAGAAAATAAGCAGGACAAGCAGGGCAAACAAGATAATCAAGTACAAT
CGGAAAATGTAGTCATAGTACCCACAGATTCACACAACTTAGATATATGGGATGAAATTTCTCTAAAGGAAATACAAGCT
GGTGAACATACAAATTTATTTGCGGAACAAAGTAATTATCAAAATATTAATTTGTTGCAAGTTAGCGATATCCGTTTATA
TTTGGACAAGCAACTACAATTCAGTTCCGTCGATGAGCAAATTTATCATGAAGCACTTGTGCATCCAATTATGTCAAAAG
TAATTGATCCAAAACGTGTTCTCATATTAGGCGGTGGCGATGGCCTTGCCCTGCGAGAAGTTTTAAAATATGAAACTGTA
CTACATGTAGACCTTGTTGACTTAGATGGATCGATGATTGATATGGCTCGTAATGTTCCTGAATTAGTTTCTTTAAACAA
AAGTGCATTTTTTGATAATCGTGTAAATACACACGTATGTGATGCAAAAGAATTTTTAAGCTCTCCTTCCTCTTTATACG
ATGTAATCATTATTGATTTTCCAGATCCAGCGACAGAGTTGTTAAGTACTTTATATACAAGCGAACTTTTTGCTCGTATA
GCTACATTTTTAACAGAAGACGGCGCGTTCGTCTGCCAATCTAATTCACCTGCTGATGCACCTCTAGTATATTGGAGTAT
CGGTAACACAATTGAACATGCGGGATTAACTGTGAAAAGTTATCATACAATCGTTCCTTCTTTCGGAACTGACTGGGGAT
TTCATATCGCTGCTAATTCTGTCTATGTACTCGATCAAATTGAGCAATTATACGTAGTACCAACTCCTCGAACATTACCT
TCCCTTCTTTTTCCTTTATTTCAATTTAAAGAAGAACATTTAGAACAACGTAATTTCGCTCTCCTAAACTCAGAATCGAA
CCTTATCCTACACCAATGTTACAAAAAGGAAATGGAGTTTTGA

Upstream 100 bases:

>100_bases
GTGAAAATCTATTAAAAAAACTCCTCTATCTATTGTCCAAGCAAACTCTACTAAATTACATAGAATAGAATAAAATAAAA
CAATTCGAAAGGGGATTCCT

Downstream 100 bases:

>100_bases
CGATATCACAGGAAGGAGTGCTACCTTATGGAATATTCTACTTTCGGTAAGCATATAATAGTAGATTTGTGGGGAGTGGA
TTTTTCCCTATTAGATGATA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2 [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPKHRKQSKIKIYRITNYKKDKRSELDSNKFELEQQAVENKQDKQGKQDNQVQSENVVIVPTDSHNLDIWDEISLKEIQA
GEHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETV
LHVDLVDLDGSMIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTLYTSELFARI
ATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANSVYVLDQIEQLYVVPTPRTLP
SLLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF

Sequences:

>Translated_360_residues
MPKHRKQSKIKIYRITNYKKDKRSELDSNKFELEQQAVENKQDKQGKQDNQVQSENVVIVPTDSHNLDIWDEISLKEIQA
GEHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETV
LHVDLVDLDGSMIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTLYTSELFARI
ATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANSVYVLDQIEQLYVVPTPRTLP
SLLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF
>Mature_359_residues
PKHRKQSKIKIYRITNYKKDKRSELDSNKFELEQQAVENKQDKQGKQDNQVQSENVVIVPTDSHNLDIWDEISLKEIQAG
EHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETVL
HVDLVDLDGSMIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTLYTSELFARIA
TFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANSVYVLDQIEQLYVVPTPRTLPS
LLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=173, Percent_Identity=30.0578034682081, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786312, Length=172, Percent_Identity=30.8139534883721, Blast_Score=81, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI212645616, Length=174, Percent_Identity=35.632183908046, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25154106, Length=173, Percent_Identity=35.8381502890173, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325326, Length=174, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323175, Length=233, Percent_Identity=30.4721030042918, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24645443, Length=171, Percent_Identity=27.4853801169591, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 41152; Mature: 41021

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHRKQSKIKIYRITNYKKDKRSELDSNKFELEQQAVENKQDKQGKQDNQVQSENVVIV
CCCCCCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEE
PTDSHNLDIWDEISLKEIQAGEHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQ
ECCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCEEEEEEECEEEEEECCCCHHHHHHH
IYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETVLHVDLVDLDGSMIDMARNVP
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEEEEEECCCHHHHHHHCCH
ELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTLYTSELFARI
HHHHCCCHHHCCCCCCCEECCHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
ATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANS
HHHHCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCEEEHHHEECCCCCCCCCEEEECCC
VYVLDQIEQLYVVPTPRTLPSLLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF
EEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PKHRKQSKIKIYRITNYKKDKRSELDSNKFELEQQAVENKQDKQGKQDNQVQSENVVIV
CCCCCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHHCCHHHHCCCCCCCCCCCCEEEE
PTDSHNLDIWDEISLKEIQAGEHTNLFAEQSNYQNINLLQVSDIRLYLDKQLQFSSVDEQ
ECCCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCEEEEEEECEEEEEECCCCHHHHHHH
IYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETVLHVDLVDLDGSMIDMARNVP
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEEEEEECCCHHHHHHHCCH
ELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTLYTSELFARI
HHHHCCCHHHCCCCCCCEECCHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
ATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANS
HHHHCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCEEEHHHEECCCCCCCCCEEEECCC
VYVLDQIEQLYVVPTPRTLPSLLFPLFQFKEEHLEQRNFALLNSESNLILHQCYKKEMEF
EEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]