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The map label for this gene is prfB [H]

Identifier: 49188023

GI number: 49188023

Start: 4908448

End: 4909428

Strand: Reverse

Name: prfB [H]

Synonym: BAS5037

Alternate gene names: 49188023

Gene position: 4909428-4908448 (Counterclockwise)

Preceding gene: 49188024

Following gene: 49188022

Centisome position: 93.89

GC content: 40.27

Gene sequence:

>981_bases
ATGATGGGCGCAGGATTTTGGGATGACCAACAAGGCGCACAAGCTGTAATTAATGAAGCGAATGCACTGAAAGATATGGT
TGGAAAGTTCCGTCAGCTAGATGAGACGTTCGAGAATCTAGAAATTACGCATGAGCTTTTAAAAGAAGAGTATGATGAAG
ATTTACATGAGGAGCTTGAATCAGAAGTAAAAGGTTTAATTCAAGAAATGAATGAGTATGAACTTCAGTTACTACTTAGC
GATCCTTATGATAAAAATAAAGCGATTTTAGAATTACACCCAGGTGCTGGTGGAACAGAGTCACAAGACTGGGGCTCTAT
GTTACTACGTATGTACACACGTTGGGCTGAAAAACGTGGATTTAAAGTAGAAACAGTTGACTACTTACCAGGTGATGAAG
CTGGTATTAAGAGTGTTACGTTATTAATTAAAGGTCATAACGCTTACGGTTACTTAAAGGCAGAGAAAGGTGTACATCGT
CTTGTACGTATTTCTCCATTCGATTCTTCAGGCCGTCGCCATACATCGTTCGTATCTTGTGAAGTTGTACCTGAGTTCAA
TGATGAAGTTGAAATTGAAGTGCGTACAGAAGACTTGAAAATTGATACGTATCGTGCAAGTGGAGCTGGTGGACAGCACG
TTAATACGACAGATTCAGCAGTTCGTATTACGCATACGCCGACAAATACGGTTGTAACGTGTCAGTCAGAGCGTTCTCAA
ATTAAAAACCGTGAGCATGCGATGAAGATGTTAAAAGCGAAATTATATCAAAAGAAATTAGAAGAGCAACAAGCGGAGTT
AGATGAAATTCGCGGAGAACAAAAGGAAATTGGATGGGGTAGTCAAATCCGTTCTTACGTATTCCACCCGTATTCTCTTG
TGAAAGACCACCGTACAAATACAGAGGTCGGTAACGTGCAAGCAGTTATGGATGGAGAAATTGACCCATTCATTGATGCT
TACTTACGTTCTCGCATCTAA

Upstream 100 bases:

>100_bases
AGACAGGAATTAGAGAAAATGGCTAAGAGATTAGCGGCTTTTAGGGGGTCTCTTTGACCTCCCTACTAAGGAGAAACAAA
TTGCAGAATTAGAAGAGAAA

Downstream 100 bases:

>100_bases
TGAGAAAAAGGCCAACAAAATTGTTGGCGCATGTTGAGGACAATAGAGGATATACAGAGTTTGATTTTATTTCTATAGAT
GAAGTAAAGAGGAAGAGAAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MMGAGFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELESEVKGLIQEMNEYELQLLLS
DPYDKNKAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCEVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQ
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDA
YLRSRI

Sequences:

>Translated_326_residues
MMGAGFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELESEVKGLIQEMNEYELQLLLS
DPYDKNKAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCEVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQ
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDA
YLRSRI
>Mature_326_residues
MMGAGFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELESEVKGLIQEMNEYELQLLLS
DPYDKNKAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCEVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQ
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDA
YLRSRI

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=324, Percent_Identity=37.3456790123457, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=35.625, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI166795305, Length=228, Percent_Identity=36.4035087719298, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI2367172, Length=318, Percent_Identity=49.3710691823899, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1787462, Length=317, Percent_Identity=37.5394321766561, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=256, Percent_Identity=35.15625, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=312, Percent_Identity=33.974358974359, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19921226, Length=264, Percent_Identity=40.1515151515151, Blast_Score=171, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37215; Mature: 37215

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGAGFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVKGLIQEMNEYELQLLLSDPYDKNKAILELHPGAGGTESQDWGSMLLRMYTRWAEKRG
HHHHHHHHHCCHHEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEHHHCCEEEEEECCCCCCCCCCCEEEEE
EVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQ
EECCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHHH
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHCCCCC
TEVGNVQAVMDGEIDPFIDAYLRSRI
CCCCCEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure
MMGAGFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVKGLIQEMNEYELQLLLSDPYDKNKAILELHPGAGGTESQDWGSMLLRMYTRWAEKRG
HHHHHHHHHCCHHEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEEECCCCCCCCCCEEEEEEECCCCEEEEEHHHCCEEEEEECCCCCCCCCCCEEEEE
EVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQ
EECCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECHHHHH
IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHCCCCC
TEVGNVQAVMDGEIDPFIDAYLRSRI
CCCCCEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]