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The map label for this gene is cysL [H]

Identifier: 49188011

GI number: 49188011

Start: 4896715

End: 4897608

Strand: Direct

Name: cysL [H]

Synonym: BAS5025

Alternate gene names: 49188011

Gene position: 4896715-4897608 (Clockwise)

Preceding gene: 49187986

Following gene: 49188012

Centisome position: 93.65

GC content: 40.04

Gene sequence:

>894_bases
ATGAACGTAGATATTTTAAAAATCTTTGTTACTGTCGTTGAGCAGAAACATTTCTCCCGTGCAGCAGAATTATTAAACCT
TTCACAGCCTGGCGTAAGTATGCATATACGCAACTTAGAAAATGAATTTGGAACTACACTTATTCAGCGCTCACCGAAGC
ACGTTCAAGTGACCGAGGCCGGAAACATCTTATATATACATGCAAAGCAAATGCTCTCTCTTTACGAAGATGCTAAGCAA
GAAATTAACGAGCTACATAACGTTGTAACAGGAACTCTTCGCATCGGTGCTAGTTTTACAATTGGCGAATACTTACTTCC
GAAAATACTAGCTGACTACGCGAATGAAAATCCACACGTCGAAGTTCACACCTTCATTTCAAATACAGAAGACGTTTTGC
AAAGCCTTCGCTCTAATCAAATTGATATCGGCTTAGTAGAAGGGCAAGTCGTATACGCTGACGTTGACGTTGAAACATTT
ATGCAAGATGAAATGAAGCTCGTCGTTCCACCAAACCATCCACTGCTCCGCACAAATAAAATAAATGAAAGGACACTCCA
AGATCAAGTATGGGTATTAAGAGAAAGTGGTTCTGGTACACGCGCTTATAGTGACCGCTTTATTCATCAACACCATTTAA
AAATGAAGCGATTTTTTACATTCAGCAGTATACAAAGCGTGAAGGAAGCGGTTAGTGCTGGGCTTGGCATCGCTATCCTT
TCTGATTGGACTGTACGGAAAGAGCTACTTGCAAAAGAACTATTCCACGTCGAAGTTCCAAATGAACAACTCATCCGACC
ATTTTCCATCGTCCGCGGCAAATATTTCATCCCATCTAAAGCTATTCAAGTGTTTTTAAATCATGTGAATTCTTTTGCGA
AAAAACAGCATTGA

Upstream 100 bases:

>100_bases
CATTATAAGAAAGTTTCTATAATAAGTTTAATACCAATACCAAATCTCTATCATCAGAAATACTTATGATAAGATGATAG
AAAAGAAAGGAGCCGATTAT

Downstream 100 bases:

>100_bases
CCTTCACATTAGTGTGAAGGTTTACAATGCATGTAAAAGGAGTGAAACACATTGCGAATTGGAGAGTTATCAAAAGAAAC
TGGCGTTAGTGAGAGATCAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNKINERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Sequences:

>Translated_297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNKINERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
>Mature_297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNKINERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=32.6315789473684, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1787879, Length=302, Percent_Identity=29.1390728476821, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=26.7796610169492, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788748, Length=235, Percent_Identity=27.2340425531915, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081904, Length=291, Percent_Identity=25.7731958762887, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI157672245, Length=223, Percent_Identity=26.457399103139, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI145693105, Length=235, Percent_Identity=23.4042553191489, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=25.7028112449799, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=27.4193548387097, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786401, Length=307, Percent_Identity=25.4071661237785, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=32.1917808219178, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787601, Length=191, Percent_Identity=25.130890052356, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788887, Length=183, Percent_Identity=30.0546448087432, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI2367136, Length=245, Percent_Identity=24.4897959183673, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788296, Length=245, Percent_Identity=24.0816326530612, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789639, Length=118, Percent_Identity=33.0508474576271, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87082132, Length=257, Percent_Identity=25.2918287937743, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790208, Length=244, Percent_Identity=23.3606557377049, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789204, Length=310, Percent_Identity=23.5483870967742, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789440, Length=118, Percent_Identity=31.3559322033898, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=25, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787128, Length=320, Percent_Identity=22.5, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789506, Length=196, Percent_Identity=23.469387755102, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=117, Percent_Identity=31.6239316239316, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=241, Percent_Identity=19.5020746887967, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=23.1372549019608, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33905; Mature: 33905

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GNILYIHAKQMLSLYEDAKQEINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCE
EVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPPNHPLLRTNK
EEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECCCCCEEECCC
INERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
CCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
CCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GNILYIHAKQMLSLYEDAKQEINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCE
EVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPPNHPLLRTNK
EEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECCCCCEEECCC
INERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKMKRFFTFSSIQSVKEAVSAGLGIAIL
CCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
CCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]