Click here to switch to the map view.

The map label for this gene is pbuE [H]

Identifier: 49187874

GI number: 49187874

Start: 4767529

End: 4768701

Strand: Reverse

Name: pbuE [H]

Synonym: BAS4885

Alternate gene names: 49187874

Gene position: 4768701-4767529 (Counterclockwise)

Preceding gene: 49187875

Following gene: 49187873

Centisome position: 91.2

GC content: 38.62

Gene sequence:

>1173_bases
TTGAATTTTCGTGTGTATATTTTAGCTATCGCGGCTTTCGTAGTCGGAACAGTTGAGCTAATTATAGGAGGAACGCTTGA
TTTAGTTGCCAATGATTTAGGAGTATCTATTAGTGCAGCTGGTCAGCTTATCACAATATTTTCAGTAGTATTTGCTTTAT
CAGGTCCTGTTTTATTAGCATTAACAGGGAAGTTTGAAAGAAAAACATTGTATGTTGGGGCATTATCAATTTTCTTAATC
GGAAATATTATTTCAGCATGTAGTGTGAATTACGGCATGTTAATGTTCTCAAGGGTGATTTGTGCGGCGAGTGGTTCGCT
TATTATTGCACTGTCAGTAACACTTGCTTCTAGCGTTGTAGAACCGCATTTTCGTGCGCGTGCAATTGGAATTATTTTCA
TGGGGATTAGTGGATCACTTGTACTTGGAGTACCGCTTGGCCTTGTACTCGGAAATGCATATGGATGGCGTGCACCATTT
GTACTCATTTCAATCTTAACAGTTATGGCAATCGCTTGTATTTCATTATTCTTAACGAAAGTACCACCGACATCGGTATT
ATCAATAAGAGAACAAATTGCTACGTTAAAAGATAAAAAAATCGTAAGTGCACAGTTAACATCATTCTTATTTTTAACGG
GCCATTTAACACTATATGCATATTTAACACCTTTCTTAAAAGATGTGATGCATGTGGAAGCGAATTGGATTAGTGTATTT
TACTTCATCTTCGGAATCGCAGCAGTAATTGGGGGCGGCTTCGGAGGCTGGCTTGCTGATAAATGGGGATCGAAGAAAAG
TATTATTTCAATTATTATCGTATTTGCGTGTGCGATCTTCCTATTGCCATTAATGACATTCTCGTTCCCGCTGTTCATTA
TTATGATGGGAATTTGGAGTATGCTGAGCTGGGCTATTTCGCCAGCACAACAAAATTATTTGATTGAAATTGCACCAGAA
TCAGCTGGTATTCAGCAAAGTTTAAATAACTCTGCCCTTCACTTAGGGATTGCGCTCGGTTCAACAGTAGGCGGAGTTGT
TATTGAGAAATCGTCTGTTATATATAATGCTTGGGTAGGAGGCGGCTTTATTATATTAGCGCTGCTTTGTGCGATTTTCT
CGATTACGAGAGGACGTTCTACTCAGTTTGCAAAAGAAGAATCTATCGTTTGA

Upstream 100 bases:

>100_bases
TACGCCTACCTTTTTTGCTGTAAAAAAATTGTACTTGTGTTCAACTTCTATTTTGGTTACTATAGTCGTAGTAACCAAAA
TAGAAAGCGTGGGGAAAGAC

Downstream 100 bases:

>100_bases
TAGGTTCTTTTTTATTTGTAAAAAATGGAAGGGTTTGGTGGGTAGATGGAGAAAGGTGTATATAGCGAGGTGATGGAAAA
TGATGTCTAATTTCTCGGTA

Product: major facilitator family transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MNFRVYILAIAAFVVGTVELIIGGTLDLVANDLGVSISAAGQLITIFSVVFALSGPVLLALTGKFERKTLYVGALSIFLI
GNIISACSVNYGMLMFSRVICAASGSLIIALSVTLASSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWRAPF
VLISILTVMAIACISLFLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANWISVF
YFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGIWSMLSWAISPAQQNYLIEIAPE
SAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVGGGFIILALLCAIFSITRGRSTQFAKEESIV

Sequences:

>Translated_390_residues
MNFRVYILAIAAFVVGTVELIIGGTLDLVANDLGVSISAAGQLITIFSVVFALSGPVLLALTGKFERKTLYVGALSIFLI
GNIISACSVNYGMLMFSRVICAASGSLIIALSVTLASSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWRAPF
VLISILTVMAIACISLFLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANWISVF
YFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGIWSMLSWAISPAQQNYLIEIAPE
SAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVGGGFIILALLCAIFSITRGRSTQFAKEESIV
>Mature_390_residues
MNFRVYILAIAAFVVGTVELIIGGTLDLVANDLGVSISAAGQLITIFSVVFALSGPVLLALTGKFERKTLYVGALSIFLI
GNIISACSVNYGMLMFSRVICAASGSLIIALSVTLASSVVEPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWRAPF
VLISILTVMAIACISLFLTKVPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANWISVF
YFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGIWSMLSWAISPAQQNYLIEIAPE
SAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVGGGFIILALLCAIFSITRGRSTQFAKEESIV

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=330, Percent_Identity=30, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787808, Length=232, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786595, Length=349, Percent_Identity=20.3438395415473, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693198, Length=234, Percent_Identity=22.2222222222222, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41566; Mature: 41566

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFRVYILAIAAFVVGTVELIIGGTLDLVANDLGVSISAAGQLITIFSVVFALSGPVLLA
CCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEE
LTGKFERKTLYVGALSIFLIGNIISACSVNYGMLMFSRVICAASGSLIIALSVTLASSVV
EECCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
EPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWRAPFVLISILTVMAIACISLFLTK
CHHHHHEEEEEEEECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANWISVF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGIWS
HHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLSWAISPAQQNYLIEIAPESAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVG
HHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCC
GGFIILALLCAIFSITRGRSTQFAKEESIV
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure
MNFRVYILAIAAFVVGTVELIIGGTLDLVANDLGVSISAAGQLITIFSVVFALSGPVLLA
CCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEE
LTGKFERKTLYVGALSIFLIGNIISACSVNYGMLMFSRVICAASGSLIIALSVTLASSVV
EECCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHH
EPHFRARAIGIIFMGISGSLVLGVPLGLVLGNAYGWRAPFVLISILTVMAIACISLFLTK
CHHHHHEEEEEEEECCCCCEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
VPPTSVLSIREQIATLKDKKIVSAQLTSFLFLTGHLTLYAYLTPFLKDVMHVEANWISVF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YFIFGIAAVIGGGFGGWLADKWGSKKSIISIIIVFACAIFLLPLMTFSFPLFIIMMGIWS
HHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLSWAISPAQQNYLIEIAPESAGIQQSLNNSALHLGIALGSTVGGVVIEKSSVIYNAWVG
HHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEECCC
GGFIILALLCAIFSITRGRSTQFAKEESIV
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]