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The map label for this gene is gcvH

Identifier: 49187849

GI number: 49187849

Start: 4743090

End: 4743473

Strand: Reverse

Name: gcvH

Synonym: BAS4859

Alternate gene names: 49187849

Gene position: 4743473-4743090 (Counterclockwise)

Preceding gene: 49187850

Following gene: 49187848

Centisome position: 90.72

GC content: 38.28

Gene sequence:

>384_bases
ATGAGCATTCCAAATAATTTACGTTACTCTGAAGAACACGAATGGGTAAAAACTGAAGGTAATGAAGTTGTTATCGGTAT
TACTCACTTTGCACAAAATGAACTAGGCGATATCGTATTCGTTGAACTTCCTGAAGTAGGTGCAACAATCGAAGCTGACG
AGCCATTCGGAAGCGTAGAATCTGTTAAAACAGTTTCTGAATTATACGCACCTGTAAGCGGTAAAGTTGTAGCAGTAAAC
GAAGAATTAAGTGACCAACCAGAACTTGTTAACGAATCTCCATACGAGGGTGCATGGATGGTTAAAGTTGAACTTTCTGA
TGCAAGCCAAGTTGAGAAGTTATTAACTGCTGAAAAATATGCAGAAATGACAAACCAAGACTAA

Upstream 100 bases:

>100_bases
AGGTAACACTTGGTTTTAACGAAGAGCAGTTTGAAAGTGTGTGGAAAAAGTACCAATAAGGTATTTTTACATAATTAATA
CATTCACTGGAGGTACAGTC

Downstream 100 bases:

>100_bases
TTATAGTCGACTGAAGGACAGCCTTTGTGCTGTCCTTTTTTATTTTGTGTTACATAATGCATTTAAAAACGAGCGCAAAT
GAATAAGCTATTATGGGAAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSIPNNLRYSEEHEWVKTEGNEVVIGITHFAQNELGDIVFVELPEVGATIEADEPFGSVESVKTVSELYAPVSGKVVAVN
EELSDQPELVNESPYEGAWMVKVELSDASQVEKLLTAEKYAEMTNQD

Sequences:

>Translated_127_residues
MSIPNNLRYSEEHEWVKTEGNEVVIGITHFAQNELGDIVFVELPEVGATIEADEPFGSVESVKTVSELYAPVSGKVVAVN
EELSDQPELVNESPYEGAWMVKVELSDASQVEKLLTAEKYAEMTNQD
>Mature_126_residues
SIPNNLRYSEEHEWVKTEGNEVVIGITHFAQNELGDIVFVELPEVGATIEADEPFGSVESVKTVSELYAPVSGKVVAVNE
ELSDQPELVNESPYEGAWMVKVELSDASQVEKLLTAEKYAEMTNQD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=46.9026548672566, Blast_Score=120, Evalue=2e-28,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=46.0176991150442, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=53.3333333333333, Blast_Score=134, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17551294, Length=123, Percent_Identity=42.2764227642276, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=44.3478260869565, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=42.1487603305785, Blast_Score=103, Evalue=6e-24,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=46.4912280701754, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_BACAA (C3PDM2)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869232.1
- ProteinModelPortal:   C3PDM2
- SMR:   C3PDM2
- EnsemblBacteria:   EBBACT00000127147
- GeneID:   7851403
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5263
- GeneTree:   EBGT00050000002634
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14045; Mature: 13913

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNNLRYSEEHEWVKTEGNEVVIGITHFAQNELGDIVFVELPEVGATIEADEPFGSVE
CCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCHH
SVKTVSELYAPVSGKVVAVNEELSDQPELVNESPYEGAWMVKVELSDASQVEKLLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
AEMTNQD
HHHCCCC
>Mature Secondary Structure 
SIPNNLRYSEEHEWVKTEGNEVVIGITHFAQNELGDIVFVELPEVGATIEADEPFGSVE
CCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEECCCCCCCHH
SVKTVSELYAPVSGKVVAVNEELSDQPELVNESPYEGAWMVKVELSDASQVEKLLTAEKY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
AEMTNQD
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA