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The map label for this gene is ag45 [H]

Identifier: 49187771

GI number: 49187771

Start: 4670978

End: 4672312

Strand: Direct

Name: ag45 [H]

Synonym: BAS4781

Alternate gene names: 49187771

Gene position: 4670978-4672312 (Clockwise)

Preceding gene: 49187769

Following gene: 49187773

Centisome position: 89.33

GC content: 37.3

Gene sequence:

>1335_bases
ATGAGAGTTTTGCACGAAACAACGCAAGAGCTCGTAAATTGGCAGTATTATTTTGCTATCGCAGTCTTTTTAATTACATA
TGCCATTATTATTTCTGAAAAAATCAATCGCGCTGTCATCGCACTTCTCGGTGCAGCACTCATGGTAATTATGGGGGTCG
TTGATTTACACAACGCCTTTACGAAACATATTGAATGGGGGACGATTACACTACTCATCGGTATGATGATTTTGGTGAAC
ATTACGAGTAAATCGGGCGTCTTCCAATACGTTGCCATTAAAGCAGCAAAAGGAGCGCAAGGAAATCCAATTAAAATTTT
AATTTCACTTTCCTTACTTACTGCGCTTGGTTCCGCATTTTTAGATAACGTTACAACTGTACTTCTTGTTGTTCCAGTTA
CTTTATCTATTACGCGCATACTGCAAGTAAATCCTGTTCCTTATTTACTTTCTGAAATTATTTTTTCAAACATTGGGGGA
ACAGCAACATTAATTGGTGATCCACCTAATATTATGATTGGTTCTGCAAATAAGCATTTAGACTTCAATGCATTCTTGAT
AAACTTAGCGCCAATCGTAATTATTATTATTGCAGTGACAGCAGTCATACTTTACTTCATGTACCGTAAACAATTAATTG
CTGATCCTGTACAAGTTAAGAAATTAATGAGCCTAGATGAAAAACAATACATTAAAGATCCAGTATTAATGAAAAAATCT
TTAACAGTACTTGGACTTACAATTGTAGGTTTCATGACTCATTCGATTTTCCACATTGATGCAGCTATCATCGCTTTAAC
TGGTGCTACTGTACTTATGCTAATTGCTGTGAAAGAACATGAATTTGAAGAGGTATTTGCAAGTGTAGAATGGGTAACAA
TCTTCTTCTTCGCAGGACTATTCGTACTCGTTGGAGGACTTATCGATATCGGCCTTATTAAAATGCTAGCTCAAAAAGTA
ATTGGCATAACAGGCGGAGATATTTCTTACGCATCTATCCTTATTTTATGGGTGTCTGGTATCGCCTCTGCAACAATTGA
TAACATCCCATTCGTTGCAACAATGATCCCACTTATTAACGATATGGCAGTTGGGCTAGGTTTATCACCTTCTGACGCAC
AAATCGACGTATTATGGTGGGCATTAGCATTAGGTGCTTGTCTGGGCGGAAACGGAACATTAATCGGAGCTTCAGCTAAC
GTAATTGTCGCAGGTATCGCAAGCCGCGAAGGACATAAATTTAGCTACATGGACTTCCTAAAAGTCGGTTTCCCAATTAT
GATCGTTTCATTAATCATTTCTCATATTTATATTTATTTACGCTACCTTATGTAG

Upstream 100 bases:

>100_bases
GCCCTTTCATTTACATCCGACAATTTTCTAATTTATAATGACGTTATGTGTGTTTCATCTAGTTAATTTTTGACACACTA
AGAAAAGTGAATGAAAGGAG

Downstream 100 bases:

>100_bases
TAGAAAAAGCGCTCTGATAATCAGGGCGCTTTTTTCATTACACATATTTAAAATAGACCTACTCGTTATACCTCACCGGC
TCATTTCGAATAATGAACAA

Product: arsenical pump family protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRVLHETTQELVNWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKHIEWGTITLLIGMMILVN
ITSKSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGG
TATLIGDPPNIMIGSANKHLDFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKS
LTVLGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEFEEVFASVEWVTIFFFAGLFVLVGGLIDIGLIKMLAQKV
IGITGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASAN
VIVAGIASREGHKFSYMDFLKVGFPIMIVSLIISHIYIYLRYLM

Sequences:

>Translated_444_residues
MRVLHETTQELVNWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKHIEWGTITLLIGMMILVN
ITSKSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGG
TATLIGDPPNIMIGSANKHLDFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKS
LTVLGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEFEEVFASVEWVTIFFFAGLFVLVGGLIDIGLIKMLAQKV
IGITGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASAN
VIVAGIASREGHKFSYMDFLKVGFPIMIVSLIISHIYIYLRYLM
>Mature_444_residues
MRVLHETTQELVNWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAFTKHIEWGTITLLIGMMILVN
ITSKSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAFLDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGG
TATLIGDPPNIMIGSANKHLDFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKS
LTVLGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEFEEVFASVEWVTIFFFAGLFVLVGGLIDIGLIKMLAQKV
IGITGGDISYASILILWVSGIASATIDNIPFVATMIPLINDMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASAN
VIVAGIASREGHKFSYMDFLKVGFPIMIVSLIISHIYIYLRYLM

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CitM (TC 2.A.11) transporter family [H]

Homologues:

Organism=Homo sapiens, GI157266326, Length=517, Percent_Identity=35.5899419729207, Blast_Score=249, Evalue=4e-66,
Organism=Escherichia coli, GI87082277, Length=424, Percent_Identity=23.5849056603774, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24581759, Length=501, Percent_Identity=35.7285429141717, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI19920704, Length=505, Percent_Identity=35.4455445544554, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI28574683, Length=501, Percent_Identity=35.7285429141717, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI28574681, Length=501, Percent_Identity=35.7285429141717, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI28574685, Length=501, Percent_Identity=35.7285429141717, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI28574678, Length=501, Percent_Identity=35.7285429141717, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI221472711, Length=495, Percent_Identity=29.0909090909091, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24581764, Length=495, Percent_Identity=29.0909090909091, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI45550608, Length=475, Percent_Identity=31.3684210526316, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI85724998, Length=474, Percent_Identity=25.1054852320675, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI62484377, Length=474, Percent_Identity=25.1054852320675, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24656008, Length=473, Percent_Identity=27.6955602536998, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802
- InterPro:   IPR004680 [H]

Pfam domain/function: PF03600 CitMHS [H]

EC number: NA

Molecular weight: Translated: 48150; Mature: 48150

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLHETTQELVNWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAF
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKHIEWGTITLLIGMMILVNITSKSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAF
HHHCCCHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHH
LDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGGTATLIGDPPNIMIGSANKHL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCC
DFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCHHHHHHH
LTVLGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEFEEVFASVEWVTIFFFAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVLVGGLIDIGLIKMLAQKVIGITGGDISYASILILWVSGIASATIDNIPFVATMIPLIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASANVIVAGIASREGHKFSYMDFL
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHH
KVGFPIMIVSLIISHIYIYLRYLM
HHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRVLHETTQELVNWQYYFAIAVFLITYAIIISEKINRAVIALLGAALMVIMGVVDLHNAF
CCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKHIEWGTITLLIGMMILVNITSKSGVFQYVAIKAAKGAQGNPIKILISLSLLTALGSAF
HHHCCCHHHHHHHHHHHHHEECCCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHH
LDNVTTVLLVVPVTLSITRILQVNPVPYLLSEIIFSNIGGTATLIGDPPNIMIGSANKHL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCC
DFNAFLINLAPIVIIIIAVTAVILYFMYRKQLIADPVQVKKLMSLDEKQYIKDPVLMKKS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCHHHHHHH
LTVLGLTIVGFMTHSIFHIDAAIIALTGATVLMLIAVKEHEFEEVFASVEWVTIFFFAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVLVGGLIDIGLIKMLAQKVIGITGGDISYASILILWVSGIASATIDNIPFVATMIPLIN
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DMAVGLGLSPSDAQIDVLWWALALGACLGGNGTLIGASANVIVAGIASREGHKFSYMDFL
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHH
KVGFPIMIVSLIISHIYIYLRYLM
HHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]