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The map label for this gene is menF [H]

Identifier: 49187742

GI number: 49187742

Start: 4634560

End: 4635954

Strand: Reverse

Name: menF [H]

Synonym: BAS4751

Alternate gene names: 49187742

Gene position: 4635954-4634560 (Counterclockwise)

Preceding gene: 49187745

Following gene: 49187741

Centisome position: 88.66

GC content: 38.35

Gene sequence:

>1395_bases
GTGATTCAAACGAAACAAATAGGCTTGCAAGAAGTTCTAAGTGCGGCCATTAAGCGTGCAACTGATGAAAAAATATTGGT
GAGCTTCGTAAAACAAATAGACTGGATGGATCCGCTTCTGTTTTATGCAGCAGGAAAAAGGATCTCATTCGAAAATAGAT
GTTATTTTGCAGACCCAGCGCAGCATGTAATATTTGCTGGAATTGGCTCTGTTTTCACTATAGCAACTGCTTCTCGCAAA
CGCTTTCAAACTGCTCGTGACGAGTGGGATAAAGTAAAAGAGAAAGCATTTGTACAAAGGGAAAAATACGAGTTTGGAAC
AGGTCCTCTTTTATTTGGTGGATTTTCATTTGACCAAGAAAAAGAGAAAACAGATTTATGGAAAGAATTTGATGATACGA
CATTTTCACTACCAACATTTTTATTAACTGTAAAAAATGAAAAAGCATGGTTAACAATGAATACATTCGTTTCAGCAACA
GATTGTGCAGAAACTCTTTATAACGAAATTGTTTCTTTAGAAGAGAAAATTTTTGAGGAGAGTAAATGTGCACTAGAAGG
ATCAAAATTAACAGTTACTTCTAAAGTAGAAGTGGATCCGAAAGGCTGGATGAAGGCCATTGAGAAAGTGCAAGATGAGA
TGAAGCAGGGGAACGTGCAGAAGGTTGTATTAGCGAGGGAGCTAAAAGTAGAGATGGATCATCATATTGATTCTGCTCTT
GTTTTAGAAGCACTCCGCATTGGGCAACCGGATTGTTACGTATTTTCTTTTGATTATAAAGGGGCATGCTTCTTAGGAGC
GACGCCAGAGAGGTTAATTCGCAAAGAAGATGAGAAGTTTACTTCGATGTGTCTTGCGGGTTCAACTAGTCATGGTCAAT
CTATAGAAGAAAGTAAGCGAAATAGTAATGCGCTTCTTCATGATGAAAAGAATTTAGCTGAACATGGTTATGTTGTGAAC
ATGATCAGGTCGGTATTAAATGAGCACTGCGAATTCGTTAATATTCCGGAAAGTCCAGGTCTATTAACAACGAAAAACTT
AATTCATTTATATACGCCTGTAGAGGCAAAAGGTGACGCGAGTCTTTTAACGATGGTAGAAGAATTACATCCAACACCAG
CTCTTGGCGGGACACCTCGTTTAGAAGCGATGAAACTAATTCGTGATGTTGAATTGTTAGACAGAGGATTATATGGTGCA
CCGATTGGCTGGATAGATGATGAAGGAAATGGTGAATTTGCGGTTGCACTTCGCTGTGGATTATTAAATGGAGAGAAAGC
ATCTTTATTTGCCGGTTGCGGAATTGTAATTGATTCAGTACCACAACTTGAATATGAAGAAACAAGTTTGAAGTTTAGAC
CGATGCTTGGTGCTTTGGAGGAATTAATGAAATGA

Upstream 100 bases:

>100_bases
TTTCTCAGAAATGCTAAACGTTGACAGGCCTTTCGTTTGAGGTGTATCTTAAAATCAAGCGTAATTATGACTATTTTTCG
CCCATAGGGGGGAGACAATT

Downstream 100 bases:

>100_bases
ACAACCATATAGAAGCATTATCATATTATTTAGGCGCGTTCGTGGATGAACTGACGCTTCTAAATGTATGTGATGTTGTC
ATTAGTCCAGGCTCACGGTC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPAQHVIFAGIGSVFTIATASRK
RFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQEKEKTDLWKEFDDTTFSLPTFLLTVKNEKAWLTMNTFVSAT
DCAETLYNEIVSLEEKIFEESKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTSHGQSIEESKRNSNALLHDEKNLAEHGYVVN
MIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDASLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGA
PIGWIDDEGNGEFAVALRCGLLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK

Sequences:

>Translated_464_residues
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPAQHVIFAGIGSVFTIATASRK
RFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQEKEKTDLWKEFDDTTFSLPTFLLTVKNEKAWLTMNTFVSAT
DCAETLYNEIVSLEEKIFEESKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTSHGQSIEESKRNSNALLHDEKNLAEHGYVVN
MIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDASLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGA
PIGWIDDEGNGEFAVALRCGLLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK
>Mature_464_residues
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPAQHVIFAGIGSVFTIATASRK
RFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQEKEKTDLWKEFDDTTFSLPTFLLTVKNEKAWLTMNTFVSAT
DCAETLYNEIVSLEEKIFEESKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTSHGQSIEESKRNSNALLHDEKNLAEHGYVVN
MIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDASLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGA
PIGWIDDEGNGEFAVALRCGLLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=242, Percent_Identity=41.7355371900826, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI87082077, Length=273, Percent_Identity=29.6703296703297, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787518, Length=272, Percent_Identity=25.7352941176471, Blast_Score=82, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=270, Percent_Identity=25.9259259259259, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52098; Mature: 52098

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPA
CCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCEECCCCCEEECCCH
QHVIFAGIGSVFTIATASRKRFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCH
KEKTDLWKEFDDTTFSLPTFLLTVKNEKAWLTMNTFVSATDCAETLYNEIVSLEEKIFEE
HHHHHHHHHHCCCCEECCEEEEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
SKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
HHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHH
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTSHGQSIEESKR
HHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHC
NSNALLHDEKNLAEHGYVVNMIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDA
CCCCEEECCHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCEEECCEEEEECCCCCCCCH
SLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGAPIGWIDDEGNGEFAVALRCG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
LLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK
CCCCCCCHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MIQTKQIGLQEVLSAAIKRATDEKILVSFVKQIDWMDPLLFYAAGKRISFENRCYFADPA
CCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHCCCEECCCCCEEECCCH
QHVIFAGIGSVFTIATASRKRFQTARDEWDKVKEKAFVQREKYEFGTGPLLFGGFSFDQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCH
KEKTDLWKEFDDTTFSLPTFLLTVKNEKAWLTMNTFVSATDCAETLYNEIVSLEEKIFEE
HHHHHHHHHHCCCCEECCEEEEEEECCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHH
SKCALEGSKLTVTSKVEVDPKGWMKAIEKVQDEMKQGNVQKVVLARELKVEMDHHIDSAL
HHHHCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHH
VLEALRIGQPDCYVFSFDYKGACFLGATPERLIRKEDEKFTSMCLAGSTSHGQSIEESKR
HHHHHHCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHC
NSNALLHDEKNLAEHGYVVNMIRSVLNEHCEFVNIPESPGLLTTKNLIHLYTPVEAKGDA
CCCCEEECCHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCEEECCEEEEECCCCCCCCH
SLLTMVEELHPTPALGGTPRLEAMKLIRDVELLDRGLYGAPIGWIDDEGNGEFAVALRCG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
LLNGEKASLFAGCGIVIDSVPQLEYEETSLKFRPMLGALEELMK
CCCCCCCHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]