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The map label for this gene is mscL [H]

Identifier: 49187563

GI number: 49187563

Start: 4473790

End: 4474188

Strand: Direct

Name: mscL [H]

Synonym: BAS4569

Alternate gene names: 49187563

Gene position: 4473790-4474188 (Clockwise)

Preceding gene: 49187562

Following gene: 49187565

Centisome position: 85.56

GC content: 32.83

Gene sequence:

>399_bases
ATGTGGAACGAGTTTAAGAAATTCGCGTTCAAAGGGAATGTAATCGATTTAGCTGTCGGGGTTGTAATCGGTGCTGCATT
CGGTAAAATCGTTAGTTCTTTAGTAAAAGATATTATTACACCATTACTTGGTATGGTATTAGGTGGCGTTGACTTTACAG
ATTTAAAAATTACATTCGGTAAATCATCTATTATGTATGGTAACTTCATCCAAACTATTTTTGATTTCTTAATTATCGCA
GCTGCTATCTTTATGTTTGTTAAAGTGTTCAACAAACTAACATCTAAAAGAGAAGAAGAAAAAGAGGAAGAAATTCCAGA
ACCAACAAAAGAAGAAGAACTTCTTGGCGAAATTCGCGACTTATTAAAACAACAAAACTCTTCTAAAGATAGAGCATAA

Upstream 100 bases:

>100_bases
CATTCCCATTTCCCCTTTATTTCTCACGCAAATTTCTATAGAATATGTTGATAGGAATTATAAATTTCATAATGAATCGT
GGAAAGGGTGGGATTAACAC

Downstream 100 bases:

>100_bases
TTGAACGGATAAAAAGGCATGTTCCTCTCGGGACATGCCTTTTTTATATGCTATAACGTTTCCGAATTCATATGAATAAA
TAAAATAATACCAGCAACTA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MWNEFKKFAFKGNVIDLAVGVVIGAAFGKIVSSLVKDIITPLLGMVLGGVDFTDLKITFGKSSIMYGNFIQTIFDFLIIA
AAIFMFVKVFNKLTSKREEEKEEEIPEPTKEEELLGEIRDLLKQQNSSKDRA

Sequences:

>Translated_132_residues
MWNEFKKFAFKGNVIDLAVGVVIGAAFGKIVSSLVKDIITPLLGMVLGGVDFTDLKITFGKSSIMYGNFIQTIFDFLIIA
AAIFMFVKVFNKLTSKREEEKEEEIPEPTKEEELLGEIRDLLKQQNSSKDRA
>Mature_132_residues
MWNEFKKFAFKGNVIDLAVGVVIGAAFGKIVSSLVKDIITPLLGMVLGGVDFTDLKITFGKSSIMYGNFIQTIFDFLIIA
AAIFMFVKVFNKLTSKREEEKEEEIPEPTKEEELLGEIRDLLKQQNSSKDRA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=131, Percent_Identity=58.0152671755725, Blast_Score=147, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14812; Mature: 14812

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNEFKKFAFKGNVIDLAVGVVIGAAFGKIVSSLVKDIITPLLGMVLGGVDFTDLKITFG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEC
KSSIMYGNFIQTIFDFLIIAAAIFMFVKVFNKLTSKREEEKEEEIPEPTKEEELLGEIRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LLKQQNSSKDRA
HHHHHCCCCCCC
>Mature Secondary Structure
MWNEFKKFAFKGNVIDLAVGVVIGAAFGKIVSSLVKDIITPLLGMVLGGVDFTDLKITFG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEC
KSSIMYGNFIQTIFDFLIIAAAIFMFVKVFNKLTSKREEEKEEEIPEPTKEEELLGEIRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LLKQQNSSKDRA
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA